DERİN ÖTEKTİK ÇÖZÜCÜNÜN Rheum ribes KÖKLERİNDEN FENOLİK BİLEŞİK EKSTRAKSİYONU ÜZERİNE ETKİSİ

Öz

Bu çalışmada ışkın bitkisi (Rheum ribes) köklerinden biyoaktif bileşik eldesinde farklı çözücü tipleri değerlendirilmiştir. Yeşil çözücü olarak kolin klorür ve laktik asit ile hazırlanan farklı molar oranda (1:1, 1:2, 1:3) derin ötektik çözücüler ve su kullanılmıştır. Toplam fenolik bileşik miktarı derin ötektik çözücü ile etanol ekstraktına benzer olarak yüksek bulunmuştur. Toplam flavonoid bileşik ekstraksiyonunda en yüksek değer etanol ile elde edilirken ikinci sırada derin ötektik çözücü gelmektedir. Antioksidan aktivite üzerine ise en etkili çözücünün derin ötektik çözücü (1:3) olduğu saptanmıştır. Ek olarak temel bileşen analizi ile farklı çözücüler kullanılarak hazırlanan ışkın ekstraktlarının biyoaktif içerik (toplam fenolik, toplam flavonoid bileşik, antioksidan aktivite) açısından benzerlik/farklılıkları değerlendirilmiştir. Sonuç olarak, ışkından biyoaktif bileşik ekstraksiyonunda organik çözücülere alternatif olarak derin ötektik çözücünün kullanımı uygun bulunmuştur.

Anahtar Kelimeler

• ultrasonik ekstraksiyon
• yeşil çözücü
• temel bileşen analizi
• ışkın
• rhubarb
• ultrasonic extraction
• green solvent
• principal component analysis

IMPACT OF DEEP EUTECTIC SOLVENT ON PHENOLIC COMPOUND EXTRACTION FROM Rheum ribes ROOTS

Öz

In this study, different solvent types were evaluated in obtaining bioactive compounds from rhubarb (Rheum ribes) roots. Deep eutectic solvents prepared with choline chloride and lactic acid in different molar ratios (1:1, 1:2, 1:3) and water were used, as green solvents The total phenolic compounds were found to be high with deep eutectic solvent similar to the ethanol extract. The highest value is obtained with ethanol for total flavonoid compound extraction, while deep eutectic solvent comes second. For the antioxidant activity, it was determined that the most effective solvent was the deep eutectic solvent (1:3). In addition, the similarities/differences in terms of bioactive compounds (total phenolic, total flavonoid compound, antioxidant activity) of rhubarb extracts prepared using different solvents were evaluated with principal component analysis. As a result, the use of deep eutectic solvent was found to be appropriate as an alternative to organic solvents in bioactive compound extraction from rhubarb.

Anahtar Kelimeler

• rhubarb
• ultrasonic extraction
• green solvent
• principal component analysis

Kaynakça

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