MAYALARDA SAFRA TUZU TOLERANSININ BELİRLENMESİNDE FARKLI SICAKLIK VE BESİYERİ KULLANIMININ ETKİSİ

Öz

Mikrobiyel karakterizasyonda deney şartları, sonuca etki eden önemli bir parametre olarak karşımıza çıkmaktadır. Bu çalışmada 50 maya izolatının, safra tuzuna toleransları 3 farklı sıcaklık (26°C, 30°C ve 37°C) ve 2 farklı besiyeri (Sabouraud-2% dekstroz broth, SDB ve Yeast Peptone Dextrose Broth, YPD) koşullarında araştırılmıştır. Genel olarak 26 ve 30 °C’de her iki besiyerinde de benzer gelişim gözlenmiştir. Fakat 37 °C’de suşların gelişimlerinde SDB besiyerinde %0.3, %0.5 ve %1 safra tuzunda sırasıyla dört, üç ve üç suş gelişirken, YPD besiyerinde dokuzar suşun geliştiği belirlenmiştir. YPD ve SDB besiyerleri maya gelişimi açısından karşılaştırıldığında, YPD'nin farklı sıcaklık ve safra oranlarında daha fazla suşun gelişimini destekleyebildiği tespit edilmiştir. Çalışmada Kazachstania unispora’nın deney koşullarından en çok etkilenen tür olduğu belirlenmiştir.

Anahtar Kelimeler

• Maya
• safra tuzu
• sıcaklık
• YPD besiyeri
• SDB besiyeri

THE EFFECT OF DIFFERENT TEMPERATURE AND MEDIA USAGE ON DETERMINING BILE SALT TOLERANCE IN YEASTS

Abstract

In microbiological characterisation, experimental conditions emerge as a significant parameter affecting the result. In this study, the tolerance of 50 yeast isolates to bile salts was investigated under three different temperature conditions (26°C, 30°C, and 37°C) and two different media (Sabouraud-2% dextrose broth and Yeast Peptone Dextrose Broth, YPD). In general, similar growth was observed in both media at 26°C and 30°C. However, at 37°C, it was determined that four, three, and three strains developed in SDB medium with 0.3%, 0.5%, and 1% bile salt, respectively, while nine strains developed in YPD medium. When YPD and SDB media were compared in terms of yeast growth, it was observed that YPD supported the growth of a greater number of strains at different temperatures and bile ratios. The study determined that Kazachstania unispora was the species most affected by the experimental conditions.

Keywords

• Yeast
• bile salt
• temperature
• YPD medium
• SDB medium

Kaynakça

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