SIĞIR KARKASLARINDAN İZOLE EDİLEN PSEUDOMONAS AERUGINOSA SUŞLARININ BİYOFİLM OLUŞTURMA ÖZELLİKLERİ İLE QUORUM SENSING VE MAJOR KARBAPENEMAZ GENLERİNİN ARAŞTIRILMASI

Öz

Bu çalışma mezbahalardaki sığır karkaslarından izole edilen ve fenotipik olarak karbapenem direnci gösteren P. aeruginosa suşlarının biyofilm oluşturma kapasitelerini kalitatif ve kantitatif yöntemlerle araştırmak, karbapenem direncinden sorumlu major karbapenemaz ve ayrıca quorum sensing (lasI, lasR, rhlI ve rhlR) genlerinin varlığı belirlemek amacıyla gerçekleştirilmiştir. Çalışmada her biri farklı bir sığır karkas yüzeyinden izole edilen ve moleküler yöntemlerle doğrulanmış 13 P. aeruginosa suşu materyal olarak kullanılmıştır. Çalışmada tüm P. aeruginosa suşlarının test edilen karbapenemlerden en az birine karşı dirençli olduğu belirlenmiştir. Karbapenemaz genleri (blaKPC, blaOXA-48, blaNDM, blaVIM ve blaIMP) hiçbir izolatta tespit edilememiştir. Karbapenem direnci gösteren tüm izolatların kantitatif olarak güçlü biyofilm üreticisi olduğu saptanmıştır. PCR analizleri doğrultusunda analiz edilen 13 izolattan 12’sinin (%92.3) lasI, lasR, rhlI ve rhlR genlerinin tümünü içerdiği tespit edilmiştir. Bir izolatın (%7.69) sadece lasR ve rhlR genlerini içerdiği belirlenmiştir. Yapılan bu çalışma ile hayvansal orjinli P. aeruginosa suşlarında karbapenem direncinin ve biyofilm üretiminin birbirleriyle ilişki içerisinde olduğu belirlenmiştir. Halk sağlığının korunması açısından çiğ etlerden kaynaklanabilecek kontaminasyona karşı dikkatli olunması tavsiye edilmektedir.

Anahtar Kelimeler

• Pseudomonas aeruginosa
• quorum sensing
• biyofilm
• karbapenem
• karkas
• mezbaha

ASSESSMENT OF BIOFILM FORMATION, QUORUM SENSING AND MAJOR CARBAPENEMASE GENES OF PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM CATTLE CARCASSES

Öz

This study was carried out to investigate the biofilm formation capacity of carbapenem resistant P. aeruginosa strains isolated from cattle carcasses by using qualitative and quantitative methods, and also to determine the presence of major carbapenemase and quorum sensing genes. In the study, 13 P. aeruginosa strains, each isolated from a different cattle carcass surface and confirmed by molecular methods, were used as materials. Determination of carbapenem resistance was carried out by disk diffusion method. The presence of blaKPC, blaOXA-48, blaNDM, blaVIM, and blaIMP genes was investigated by PCR. Biofilm production was determined by qualitative and quantitative methods. In order to detect quorum sensing systems, the presence of lasI, lasR, rhlI, and rhlR genes was investigated by PCR. In the study, all P. aeruginosa strains were found to be resistant to at least one of the carbapenems tested. Carbapenemase genes could not be detected in any isolate. All isolates showing carbapenem resistance were found to be quantitatively strong biofilm producers. According to PCR analyses, 12 of the 13 isolates analyzed (92.3%) were found to contain all lasI, lasR, rhlI and rhlR genes. It was determined that one isolate (7.69%) contained only lasR and rhlR genes. With this study, it was determined that carbapenem resistance and biofilm production in P. aeruginosa strains of animal origin are related.

Anahtar Kelimeler

• Pseudomonas aeruginosa
• quorum sensing
• biofilm
• carbapenem
• carcass
• slaughterhouse

Kaynakça

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