MICROBIAL CHARACTERIZATION OF VAN HERBY CHEESE USING MALDI-TOF MS AND EVALUATION OF STARTER CULTURE POTENTIAL OF SELECTED LACTIC ACID BACTERIA

Öz

This study examined the microbial and biochemical properties of Van Herby Cheeses, produced using traditional dry salting and brining methods. Biochemical analyses showed that dry salted cheeses contained 54.41±4.80% dry matter, 0.74±0.09 water activity, pH 5.11±0.76, 30.4±5.32% fat, and 5.81±1.20% salt, while brined cheeses had 62.60±3.20%, 0.81±0.03, 4.68±0.57, 29.0±8.22%, and 7.58±0.98%, respectively. Microbial identification using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) revealed a predominance of Enterococcus, Lactobacillus, Leuconostoc, and Bacillus species, as well as yeasts such as Debaryomyces hansenii and Candida zeylanoides. A total of 17 LAB isolates were assessed for acidification, proteolytic activity, diacetyl production, antibacterial effects, and antibiotic resistance. Among these, E. faecium S431 and L. brevis B122 exhibited strong acidifying capacity and notable antimicrobial properties, highlighting their promising potential as functional starter cultures for traditional cheese production.

Anahtar Kelimeler

• Van herby cheese
• MALDI-TOF MS
• Microbiota
• Lactic Acid Bacteria
• Starter Culture

Destekleyen Kurum

Bolu Abant İzzet Baysal University Scientific Research Projects

Proje Numarası

2022-10-01-1569

Teşekkür

All authors would like to thank Scientific Industrial and Technological Application and Research Center (SITARC).

VAN OTLU PEYNİRİNİN MALDI-TOF MS YÖNTEMİ İLE MİKROBİYAL KARAKTERİZASYONU VE SEÇİLİ LAKTİK ASİT BAKTERİLERİNİN STARTER KÜLTÜR POTANSİYELİNİN DEĞERLENDİRİLMESİ

Öz

Bu çalışmada, geleneksel yöntemle basma ve salamura olarak üretilen Van Otlu Peynirlerinin mikrobiyal ve biyokimyasal özelliklerinin tespit edilmesi ve izole edilen bazı laktik asit bakterilerinin (LAB) starter kültür olma potansiyelleri değerlendirilmiştir. Çalışmada basma ve salamura peynirlerin kuru madde, su aktivitesi, pH, yağ ve tuz içerikleri sırasıyla %54.41±4.80-%62.60±3.20, %0.74±0.09-%0.81±0.03, %5.11±0,76-%4.68±0.57, %30.4±5.32-%29.0±8.22 ve %5.81±1.20-%7.58±0.98 olarak tespit edilmiştir. İzole edilen mikroorganizmalar MALDI-TOF MS yöntemi ile tür düzeyinde tanımlanmıştır. Örneklerde ağırlıklı olarak Enterococcus, Lactobacillus, Leuconostoc ve Bacillus cinslerine ait bakteri türleri belirlenmiş ve Debaryomyces hansenii ve Candida zeylanoides gibi maya türlerine de rastlanmıştır. Seçilen 17 LAB suşunun asidifikasyon yetenekleri, proteinaz ve diasetil üretim kapasiteleri ile antibakteriyel aktiviteleri ve antibiyotik duyarlılıkları değerlendirilmiştir. Özellikle E. faecium S431 ve L. brevis B122 suşları yüksek asidifikasyon ve antimikrobiyal aktiviteleriyle öne çıkmıştır. Çalışma sonuçları, Van Otlu Peynirinin çeşitlilik arz eden mikrobiyotasını ortaya koymakta ve izole edilen suşların starter kültür olarak kullanılabileceğini göstermektedir.

Anahtar Kelimeler

• Van otlu peynir
• MALDI-TOF MS
• Mikrobiyota
• Laktik Asit Bakterileri

Proje Numarası

2022-10-01-1569

Kaynakça

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