COMPARATIVE ASSESSMENT OF NUTRITIONAL, ANTIOXIDANT, AND ANTIPROLIFERATIVE ACTIVITY OF FIRIK (FREEKEH) WHEAT

Öz

Climate change and population growth threaten agricultural yields, highlighting the need for low-input, nutrient-rich grains. Firik, obtained from immature durum wheat, offers functional and sustainable benefits, reducing pre-harvest sprouting damage. In this study, nutritional composition, phenolic content, antioxidant, and antiproliferative activity of raw and cooked firik were analyzed and compared to mature wheat. Cooked firik had slightly lower energy, ash, protein, carbohydrate, and fiber than raw firik. Firik was found to have a higher Nutrient-Rich Food index 9.3 (NRF9.3) score than bread wheat, durum wheat, and rice. The DPPH and ABTS antioxidant activity was found to be higher in raw firik than in mature wheat (P=0.027 and P=0.039, respectively). Raw and cooked firik exhibited a stronger cytotoxic response against A549 lung cancer cells compared to mature wheat (48.94%, 35.43%, and 90.32% at 500 μg/mL, respectively). This study demonstrated that firik exhibits higher antioxidant activity and stronger antiproliferative effects compared to mature wheat.

Anahtar Kelimeler

• Freekeh
• wheat
• antioxidant activity
• cytotoxicity

FİRİK (FREEKEH) BUĞDAYININ BESİNSEL, ANTİOKSİDAN VE ANTİPROLİFERATİF AKTİVİTESİNİN KARŞILAŞTIRMALI DEĞERLENDİRMESİ

Öz

İklim değişikliği ve nüfus artışı tarımsal verimi tehdit ederek, düşük girdi gerektiren, besin değeri yüksek tahıllara olan ihtiyacın önemini ortaya koymaktadır. Olgunlaşmamış durum buğdayından elde edilen firik, hasat öncesi filizlenmeye bağlı hasarı azaltarak fonksiyonel ve sürdürülebilir faydalar sunmaktadır. Bu çalışmada, çiğ ve pişmiş firiğin besin ögesi bileşimi, fenolik içeriği, antioksidan ve antiproliferatif aktivitesi analiz edilerek olgun buğdayla karşılaştırılmıştır. Pişmiş firik, çiğ firik ile karşılaştırıldığında nispeten daha düşük enerji, kül, protein, karbonhidrat ve lif içeriğine sahip olduğu görülmüştür. Firiğin, ekmeklik buğday, makarnalık buğday ve pirince kıyasla daha yüksek Besin Ögesinden Zengin Gıda İndeksi (NRF9.3) puanına sahip olduğu bulunmuştur. DPPH ve ABTS antioksidan aktivitesinin çiğ firikte olgun buğdaydan daha yüksek olduğu saptanmıştır (sırasıyla P=0,027 ve P=0,039). Çiğ ve pişmiş firik olgun buğdaya kıyasla A549 akciğer kanser hücresine karşı daha güçlü sitotoksik yanıt sergilemiştir (sırasıyla 500 μg/mL'de %48,94, %35,43 ve %90,32). Bu çalışma, firiğin olgun buğdaya kıyasla daha yüksek antioksidan aktivite ve güçlü antiproliferatif etki gösterdiğini ortaya koymuştur.

Anahtar Kelimeler

• Firik
• buğday
• antioksidan aktivite
• sitotoksisite

Kaynakça

1. AOAC. (1990). Official Methods of Analysis: Official Method of Analysis N° 934.06, 960.52, 960.39, 962.09, 923.08 and 985.29 (15. Baskı). Association of Official Analytical Chemists, Arlington.
2. AOAC. (1995). Official Methods of Analysis: Official Method for Moisture, Method No. 925.10. Association of Official Analytical Chemists, Washington DC.
3. AOAC. (2005). Official Methods of Analysis: Ash of Flour (Direct Method), Method 923.03 (18. Baskı). International Publisher, Gaithersburg.
4. Aune, D., Norat, T., Romundstad, P., Vatten, L. J. (2013). Whole grain and refined grain consumption and the risk of type 2 diabetes: a systematic review and dose-response meta-analysis of cohort studies. European Journal of Epidemiology, 28(11), 845-858. https://doi. org/10.1007/s10654-013-9852-5
5. Bach Knudsen, K. E. (2015). Microbial degradation of whole-grain complex carbohydrates and impact on short-chain fatty acids and health. Advances in Nutrition, 6(2), 206-213. https://doi.org/10.3945/an.114.007450
6. Barros Santos, M. C., Ribeiro da Silva Lima, L., Ramos Nascimento, F., Pimenta do Nascimento, T., Cameron, L. C., & Simões Larraz Ferreira, M. (2019). Metabolomic approach for characterization of phenolic compounds in different wheat genotypes during grain development. Food Research International, 124, 118-128. https://doi. org/10.1016/j.foodres.2018.08.034
7. Bayram, M. (2008). An Analysis of Scorched Immature Wheat: Frekeh. Cereal Foods World, 53(3), 134-138. https://doi.org/10.1094/CFW-53-3-0134
8. Biddulph, T. B., Plummer, J. A., Setter, T. L., Mares, D. J. (2007). Influence of high temperature and terminal moisture stress on dormancy in wheat (Triticum aestivum L.). Field Crops Research, 103(2), 139-153. https://doi.org/10.1016/j.fcr.2007.05.005

9. Blois, M. S. (1958). Antioxidant Determinations by the Use of a Stable Free Radical. Nature, 181(4617), 1199-1200. https://doi. org/10.1038/1811199a0
10. Boggini, G., Namoune, H., Abecassis, J., Cuq, B. (2012). Other Traditional Durum-Derived Products. Sissons, M., Abecassis, J., Marchylo, B., & Carcea, M. (Editörler), Durum Wheat (2. Baskı) içinde (s. 177-199). AACC International Press.
11. Boukid, F. (2021). Cereal-Derived Foodstuffs from North African-Mediterranean: From Tradition to Innovation. Boukid, F. (Editör), Cereal-Based Foodstuffs: The Backbone of Mediterranean Cuisine içinde (s. 117-150). Springer International Publishing.
12. Carsanba, E., Akca, I., Timur, M. (2017). Hatay Bölgesinde Üretilen Firiklerin Besinsel Özelliklerinin İncelenmesi. GIDA, 42(6), 726-730. https://doi.org/10.15237/gida.GD17064
13. Chao, S., Mitchell, J., Prakash, S., Bhandari, B., Fukai, S. (2022). Effect of Early Harvest and Variety Difference on Grain Yield and Pasting Properties of Brown Rice. Crops, 2(1), 23-39. https://doi. org/10.3390/crops2010003
14. Chatenoud, L., Tavani, A., La Vecchia, C., Jacobs, D. R., Negri, E., Levi, F., & Franceschi, S. (1998). Whole grain food intake and cancer risk. International Journal of Cancer, 77(1), 24-28. https://doi. org/10.1002/(sici)1097-0215(19980703)77:1<24::aid-ijc5>3.0.co;2-1
15. Djahida, B., Mahfoudi, R., Benalia, M., Djeridane, A., Ami, Y., & Yousfi, M. (2023). Inter- and intraspecific variability of phenolic content, antioxidant activities and α-amylase inhibitory potential of different bran and husk extracts from Algerian durum wheat (Triticum turgidum Desf.), barley (Hordeum vulgare L.) and their derived products (frik and mermez). Journal of Food Measurement and Characterization, 18, 1-17. https://doi.org/10.1007/s11694-023-02240-9
16. Drewnowski, A. (2009). Defining nutrient density: development and validation of the nutrient rich foods index. Journal of the American College of Nutrition, 28(4), 421s-426s. https://doi.org/10.1080/0731 5724.2009.10718106
17. Drezner, G., Dvojkovic, K., Horvat, D., Novoselović, D., Lalic, A. (2007). Environmental impacts on wheat agronomic and quality traits. Cereal Research Communications, 35, 357-360. https://doi.org/10.1556/CRC.35.2007.2.48
18. Gähler, A., Trufa, D. I., Chiriac, M. T., Tausche, P., Hohenberger, K., Brunst, A. K., & Finotto, S. (2022). Glucose-Restricted Diet Regulates the Tumor Immune Microenvironment and Prevents Tumor Growth in Lung Adenocarcinoma. Frontiers in Oncology, 12, 873293. https://doi.org/10.3389/fonc.2022.873293 Global Freekeh Market. (2021). Market Research Report. https://www. databridgemarketresearch.com/reports/global-freekeh-market
19. He, H. J., Li, G., Obadi, M., Ou, X. (2025). Recent Advances in Heat-Induced Wheat Protein Modifications. Food Chemistry: X, 29, 102625. https://doi.org/10.1016/j.fochx.2025.102625
20. Hildreth, A. C., Brown, G. B. (2020). Modification of Lane-Eynon Method For Sugar Determination. Journal of Association of Official Agricultural Chemists, 25(3), 775-778. https://doi.org/10.1093/jaoac/25.3.775
21. Jacobs, D. R., Marquart, L., Slavin, J., Kushi, L. H. (1998). Whole-grain intake and cancer: an expanded review and meta-analysis. Nutrition and Cancer, 30(2), 85-96. https://doi.org/10.1080/01635589809514647
22. Kim, M. J., Kim, S. S. (2016). Antioxidant and antiproliferative activities in immature and mature wheat kernels. Food Chemistry, 196, 638-645. https://doi.org/10.1016/j.foodchem.2015.09.095
23. Kim, M. J., Yoon, W. J., Kim, S. S. (2016). Phytochemical Compositions of Immature Wheat Bran, and Its Antioxidant Capacity, Cell Growth Inhibition, and Apoptosis Induction through Tumor Suppressor Gene. Molecules, 21(10), 1292. https://doi.org/10.3390/molecules21101292
24. Lee, S. C., Prosky, L., Vries, J. W. D. (2020). Determination of Total, Soluble, and Insoluble Dietary Fiber in Foods—Enzymatic-Gravimetric Method, MES-TRIS Buffer: Collaborative Study. Journal of AOAC INTERNATIONAL, 75(3), 395-416. https://doi. org/10.1093/jaoac/75.3.395
25. Liu, X., Huang, S., Chao, C., Yu, J., Copeland, L., Wang, S. (2021). Changes of starch during thermal processing of foods: Current status and future directions. Trends in Food Science & Technology, 119, 103524. https://doi.org/10.1016/j.tifs.2021.12.011
26. Mathankumar, M., Tamizhselvi, R., Manickam, V., Purohit, G. (2015). Assessment of Anticarcinogenic Potential of Vitex trifolia and Triticum aestivum Linn by In Vitro Rat Liver Microsomal Degranulation. Toxicology International, 22(1), 114-118. https://doi. org/10.4103/0971-6580.172269
27. Merendino, N., D’Aquino, M., Molinari, R., Gara, L. D., D’Egidio, M. G., Paradiso, A., & Tomassi, G. (2006). Chemical characterization and biological effects of immature durum wheat in rats. Journal of Cereal Science, 43(2), 129-136. https://doi.org/10.1016/j.jcs.2005.09.002
28. Merrill, A. L., Watt, B. K. (1973). Energy Value of Foods: Basis and Derivation. Agriculture Handbook No. 74, ARS USDA, Washington DC.
29. Muluh, T. A., Shu, X. S., Ying, Y. (2023). Targeting cancer metabolic vulnerabilities for advanced therapeutic efficacy. Biomedicine & Pharmacotherapy, 162, 114658. https://doi.org/10.1016/j. biopha.2023.114658
30. Musselman, L., Al-Mouslem, A. (2001). Triticum Durum in Northern Syria—Parched Corn (Frikeh) of the Bible? Economic Botany, 55, 187-189. https://doi.org/10.1007/BF02864557
31. Özberk, F., Martínez-Moreno, F., Kuzmanović, L., Ceoloni, C., Özberk, İ. (2023). Relaunching a Traditional Durum Wheat Product: New Cultivars and Introgression Lines Identified for Frike Making in Turkey. Foods, 12(16), 3037. https://doi.org/10.3390/foods12163037
32. Ozboy, O., Ozkaya, B., Ozkaya, H., Köksel, H. (2001). Effects of wheat maturation stage and cooking method on dietary fiber and phytic acid contents of firik, a wheat-based local food. Die Nahrung, 45(5), 347-349. https://doi.org/10.1002/1521-3803(20011001)45:5<347::AID-FOOD347>3.0.CO;2-T
33. Ozgen, M., Reese, R. N., Tulio, A. Z., Scheerens, J. C., Miller, A. R. (2006). Modified 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) method to measure antioxidant capacity of Selected small fruits and comparison to ferric reducing antioxidant power (FRAP) and 2,2’-diphenyl-1-picrylhydrazyl (DPPH) methods. Journal of Agricultural and Food Chemistry, 54(4), 1151-1157. https://doi. org/10.1021/jf051960d
34. Özkaya, B., Özkaya, H., Eren, N., Ünsal, A. S., Köksel, H. (1999). Effects of wheat maturation stage and cooking method on physical and chemical properties of firiks. Food Chemistry, 66(1), 97-102. https://doi. org/10.1016/S0308-8146(98)00249-0
35. Özkaya, B., Turksoy, S., Özkaya, H., Baumgartner, B., Özkeser, İ., & Köksel, H. (2018). Changes in the functional constituents and phytic acid contents of firiks produced from wheats at different maturation stages. Food Chemistry, 246, 150-155. https://doi.org/10.1016/j. foodchem.2017.11.022
36. Paradiso, A., Cecchini, C., Gara, L. D., Maria, G. D. E. (2006). Functional, antioxidant and rheological properties of meal from immature durum wheat. Journal of Cereal Science, 43(2), 216-222. https://doi.org/10.1016/j.jcs.2005.04.009
37. Pekkirişci, B., Bilgiçli, N., Cankurtaran Kömürcü, T. (2023). Determination of the physical, chemical and antinutritional properties of firik and einkorn bulgur. Cereal Chemistry, 100(5), 751-762. https://doi.org/10.1002/cche.10674
38. Ragaee, S., Seetharaman, K., Abdel-Aal el, S. M. (2014). The impact of milling and thermal processing on phenolic compounds in cereal grains. Critical Reviews in Food Science and Nutrition, 54(7), 837-849. https://doi.or g/10.1080/10408398.2011.610906
39. Rheeno, L., Yong-Suk, K. (2022). Comparison of Dietary Fiber and Free Sugar Content Between Raw and Cooked Cereal Grains. Journal of Food Hygiene and Safety, 37(5), 332-338. https://doi.org/10.13103/JFHS.2022.37.5.332
40. Singleton, V. L., Joseph A. Rossi, J. (1965). Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents. American Journal of Enology and Viticulture, 16(3), 144-158. https://doi. org/10.5344/ajev.1965.16.3.144
41. Slavin, J. L., Jacobs, D., Marquart, L. (2000). Grain Processing and Nutrition. Critical Reviews in Food Science and Nutrition, 40(4), 309-326. https://doi.org/10.1080/10408690091189176
42. Sluik, D., Streppel, M. T., van Lee, L., Geelen, A., Feskens, E. J. (2015). Evaluation of a nutrient-rich food index score in the Netherlands. Journal of Nutritional Science, 4, e14. https://doi.org/10.1017/jns.2015.4
43. Süzer, Ö., Özkanlı, O. (2020). Bölge Mutfaklarının Kullanılan Malzemeler Bağlamında Değerlendirilmesi: Gaziantep Yemekleri Üzerine Bir İnceleme. Safran Kültür ve Turizm Araştırmaları Dergisi, 3(2), 117-138.
44. Sznarkowska, A., Kostecka, A., Meller, K., Bielawski, K. P. (2017). Inhibition of cancer antioxidant defense by natural compounds. Oncotarget, 8(9), 15996-16016. https://doi.org/10.1832/oncotarget.13723
45. Takruri, H. R., Humeid, M. A., Umari, M. A. H. (1990). Protein quality of parched immature durum wheat (Frekeh). Journal of the Science of Food and Agriculture, 50(3), 319-327. https://doi.org/10.1002/jsfa.2740500305
46. TURKOMP. (2024). Türkiye Ulusal Gıda Kompozisyon Veri Tabanı. https://turkomp.tarimorman.gov.tr/ UNDP. (2018). Freekeh in Lebanon Value Chain Assessment and Analysis. UNHCR Operational Data Portal. https://data.unhcr.org/en/documents/details/66818
47. USDA. (2024). FoodData Central. U.S. Department of Agriculture, Agricultural Research Service. https://fdc.nal.usda.gov/
48. Warburg, O. (1956). On the origin of cancer cells. Science, 123(3191), 309-314. https://doi.org/10.1126/science.123.3191.309
49. Wu, F., Tian, F., Jin, Z., Liu, J., Jin, W., Chen, X., & Shen, W. (2021). Antioxidant capacities of heat‐treated wheat germ and extruded compounded bran. Cereal Chemistry, 98(3), 567-578. https://doi. org/10.1002/cche.10400
50. Yang, X., Yang, Y., Zhou, R., Bian, L. (2001). Determination of total, soluble and insoluble dietary fiber in foods by enzymatic-gravimetric method. Wei Sheng Yan Jiu, 30(6), 377-378.
51. Yilmaz, M. Yıldırım, A. (2020). Firik Üretim Teknikleri ve Fonksiyonel Özellikleri. Harran Üniversitesi Mühendislik Dergisi, 5(2), 109-121. https://doi.org/10.46578/humder.773680
52. Yilmaz, V. A. (2019). Effects of different cooking and drying methods on phenolic acids, carotenoids, and antioxidant activity of emmer (Triticum turgidum ssp. dicoccum) bulgur. Cereal Chemistry, 96(6), 1093-1102. https://doi.org/10.1002/cche.10219
53. Zeng, Z., Liu, C., Luo, S., Chen, J., Gong, E. (2016). The Profile and Bioaccessibility of Phenolic Compounds in Cereals Influenced by Improved Extrusion Cooking Treatment. PLoS One, 11(8), e0161086. https://doi.org/10.1371/journal.pone.0161086
54. Zhang, C., Guo, X., Guo, R., Zhu, L., Qiu, X., Yu, X., & Feng, Z. (2023). Insights into the effects of extractable phenolic compounds and Maillard reaction products on the antioxidant activity of roasted wheat flours with different maturities. Food Chemistry: X, 17, 100548. https://doi.org/10.1016/j.fochx.2022.100548
55. Zhang, K., Zhang, Y., Xu, N., Yang, X., Zhang, G., Zhang, Y., & Liu, Q. (2020). Study of the protein, antioxidant activity, and starch during in vitro simulated digestion of green wheat and wheat cooked flours. International Journal of Food Properties, 23(1), 722-735. https://doi.org/1 0.1080/10942912.2020.1754234