BEBEK MAMALARINDA DİREKT PCR YÖNTEMİ İLE CRONOBACTER SPP. TESPİTİ

Öz

Cronobacter spp., bebeklerde ve çocuklarda hayati tehlike yaratan menenjit, sepsis ve nekrotizanenterokolit enfeksiyonlarının önemli bir sebebidir. Çeşitli klinik vakalarda Cronobacter spp. enfeksiyonuile bebek maması tüketimi epidemiyolojik olarak ilişkili bulunmuştur. Bu araştırmada bebek mamasındapatojenin hızlı tanısı için direkt PCR yöntemi üzerine modifikasyon çalışmaları yapılmıştır. Bebekmamasında direkt PCR yöntemi ile 102KOB/mL düzeyinde ve 4 saatlik ön zenginleştirme sonrasındauygulanan PCR yöntemi ile 1 KOB/mL seviyesinde Cronobacter spp. belirlenebilmiştir. Cronobacterspp. tespitinde uygulanan PCR yönteminin kültürel belirleme yöntemlerine oranla daha hızlı ve dahaduyarlı olduğu saptanmıştır

Anahtar Kelimeler

• Cronobacter spp., bebek maması, izolasyon, PCR

DETECTION OF CRONOBACTER SPP. IN POWDERED INFANT FORMULA BY PCR

Öz

Cronobacter spp. causes meningitis, sepsis, and necrotizing enterocolitis in neonates and infants.Cronobacter spp. infections have been epidemiologically associated with consumption of reconstitutedpowdered infant formula. In this study, the modified PCR method for the rapid detection of Cronobacterspp. were performed. Detection limit of PCR assay for Cronobacter spp. was determined to be 102CFU/mL directly and 1 CFU/mL after 4-h of enrichment step in infant formula. The result showed thatthe modified PCR based technique described in this study was found more rapid and sensitive than theconventional methods for detection of Cronobacter spp. from infant formula

Anahtar Kelimeler

• Cronobacter spp., infant formula, isolation, PCR

Kaynakça

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