FRACTIONATION OF HAZELNUT POLYPHENOLIC COMPOUNDS BY USING ADSORBENT FEATURED COLUMN PACKING MATERIAL AND CHARACTERIZATION OF OBTAINED FRACTIONS

Öz

The aim of this study is fractionation of hazelnut polyphenols and their characterization. For this aim, phenolic compounds were extracted with 80 % (v/v) acetone and loaded to column. Low molecular weight (LMW) and high molecular weight fractions (HMW) were fractionated with ethanol and acetone, respectively. Furthermore, LMW fraction was fractionated into four parts according to peaks observed at UV (280nm). FractionV had the highest contents/activities; total phenolics and condensed tannins contents were 77.9 mg gallic acid equivalent (GAE)/g extract and 227 mg catechin equivalent (CE)/ g extract, respectively. Antioxidant activities of FractionV were 0.047 mg/mL IC50 value, 1.442 mmol Trolox equivalent (TE)/g extract, 307 mg FeSO4.7H2O/g extract for DPPH, ABTS and FRAP assays, respectively. As a result, it is seen that further fractionation of LMW is not needed and separation of LMW from HMW fraction is enough. HMW fraction could be utilized as a potential source of polyphenols.

Anahtar Kelimeler

• Antioxidant activity,hazelnut,fractionation,condensed tannins,total phenolics content

FINDIĞIN POLİFENOLİK MADDELERİNİN ADSORBAN ÖZELLİKTE KOLON DOLGU MADDESİ KULLANILARAK FRAKSİYONLARINA AYRILMASI VE ELDE EDİLEN FRAKSİYONLARIN KARAKTERİZASYONU

Öz

Bu çalışmanın amacı, fındığın içerdiği polifenolik maddelerin fraksiyonlarKına ayrılması ve elde edilen fraksiyonların karakterizasyonlarının gerçekleştirilmesidir. Bu amaçla, fındığın fenolik maddeleri %80 (v/v) asetonla ekstrakte edilerek kolona verilmiştir. Düşük molekül ağırlıklı (LMW) polifenolik maddeler etanol, yüksek molekül ağırlıklı (HMW) polifenolik maddeler ise aseton kullanılarak fraksiyonlanmıştır. Ayrıca LMW fraksiyonu, UV’de (280 nm’de) verdiği pik noktalarına göre dört ayrı fraksiyona ayrılmıştır. Sonuçlar değerlendirildiğinde, tüm analizlerde en yüksek değerlere sahip olan Fraksiyon V’in toplam fenolik madde ve kondense tanen miktarları sırasıyla, 77.9 mg gallik asit eşdeğer (GAE)/g ekstre ve 227 mg kateşin eşdeğer (KE)/ g ekstre; antioksidan kapasite değerleri DPPH, ABTS ve FRAP yöntemleri için sırasıyla 0.047 mg/mL IC50 değeri, 1.442 mmol Troloks Eşdeğer (TE)/ g ekstre, 307 mg FeSO4.7H2O/g ekstre olarak tespit edilmiştir. Çalışma sonucunda, LMW fraksiyonlarının ayrı fraksiyonlar olarak toplanmasına gerek olmadığı, HMW fraksiyonundan ayrılmasının yeterli olduğu tespit edilmiştir. HMW fraksiyonu, yüksek tanen içeriğiyle polifenolik maddeler açısından önemli bir kaynak olarak değerlendirilebilir.

Anahtar Kelimeler

• Fraksiyonlama,fındık,antioksidan aktivite,kondense taninler,toplam fenolik madde

Kaynakça

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