Morphological Characterization of Streptococcus Thermophilus and Lactobacillus Delbrueckii Subsp. Bulgaricus Virulent Phages (in English)

Öz

In this study, 25 phages of S. thermophilus and 25 phages of L. bulgaricus were inspected and identified morphologically by electron microscopy. In S. thermophilus phages the diameters of hexagonal heads were found to range between 47-74 nm, while non-contractile tails were 182 to 290 nm long and 7-14 nm wide. In these phages tail plaque, collar and fiber like structures were not found. Identified S. thermophilus phages were placed in the Siphoviridae family according to Ackermann’s and/or Group B in Bradley’s classification. Also for L. bulgaricus phages isometric hexagonal capsid and non-contractile tail structures were also determined. The capsids measured between 47-73 nm in diameter, and the tails were between 117-162 nm long and 7 to 13 nm wide. These phages were also placed in Siphoviridae family in Ackermann’s classification and/or group B in Bradley’s. Importantly, it was also determined that in some of these phages collar and tail plaque structures were present. It is thought that minor modifications in the preparation of the S. thermophilus and L. bulgaricus phages, and the type of electron microscope used were significant factors that affected the visibility of the tail structures.

Anahtar Kelimeler

• S. thermophilus, L. bulgaricus, phage, morphological characterization, electron microscope

Streptococcus Thermophilus ve Lactobacillus Delbrueckii Subsp. Bulgaricus Virülent Fajlarının Morfolojik Karakterizasyonu (İngilizce)

Öz

Bu çalışmada 25 adet S. thermophilus ve 25 adet L. bulgaricus fajının elektron mikroskobik incelemesi yapılarak morfolojik karakterizasyonu gerçekleştirilmiştir. S. thermophilus fajlarında izometrik, hegzagonal baş çapının 53-74 nm, kontraktil olmayan kuyruk uzunluğunun 182-290 nm ve kuyruk genişliğinin de 7-14 nm arasında değiştiği görülmüştür. Bu fajlarda yaka, kuyruk plağı ve fibril benzeri yapıya rastlanmamıştır. İncelenen tüm fajlar, elde edilen verilere dayanılarak diğer S. thermophilus fajları gibi Ackermann sınıflaması Siphoviridae familyasına ve/veya Bradley sınıflaması B grubuna dâhil edilmiştir. S. thermophilus fajlarında olduğu gibi Lb. bulgaricus fajlarında da izometrik, hegzagonal kapsit ve kontraktil olmayan kuyruk yapısı belirlenmiştir. Kapsit çapları 47-73 nm arasında değişirken, kontraktil olmayan kuyruk uzunlukları 117-162 nm ve kuyruk enleri 7-13 nm arasında bulunmuştur. Ackermann sınıflaması Siphoviridae familyasına ve/veya Bradley sınıflaması B grubuna dâhil edilen bu fajlarda yaka, kuyruk tablası ve fibril yapısının varlığı dikkat çekmiştir. S. thermophilus ve L. bulgaricus faj örneklerinin hazırlanmasındaki farklılıkların ve kullanılan elektron mikroskop tiplerinin kuyruk yapılarının görünebilirliğini etkilediği düşünülmüştür.

Anahtar Kelimeler

• S. thermophilus, L. bulgaricus, faj, morfolojik karakterizasyon, elektron mikroskop

Kaynakça

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