ŞEKER PANCARI KÜSPESİ VE MISIR KOÇANININ ENZİMATİK HİDROLİZİNDE FARKLI FAKTÖRLERİN ETKİSİNİN İNCELENMESİ: DENEY SONUÇLARININ İSTATİSTİKSEL ANALİZLERİ

Öz

Bu çalışmada, lignince düşük bir biyokütle olarak şeker pancarı küspesinin (SBP) ve lignince yüksek bir biyokütle olarak mısır koçanının (CC) enzimatik hidrolizinden elde edilecek indirgen şeker veriminde, çeşitli değişkenlerin göstereceği etkiler araştırılmıştır. SBP hidrolizinde, çeşitli parametrelerin (substrat, pektinaz, selülaz ve hidroliz süresi) şeker verimi modeline önemini belirlemek için tepki yüzeyi metodolojisi (RSM) ve ANOVA kullanılmıştır. Önerilen ikinci dereceden model, tüm ana etkilerin ve bazı etkileşim etkilerinin önemini gösteren yeterli bir yaklaşıklık vermiştir (P < 0.05). Tasarım alanı içindeki maksimum verimler, %20 substrat yüklemesinde 300 µl Cellic Ctec3 ve 300 µl Pectinex Ultra SP-L enzimleri kullanılarak 18 saatlik hidrolizden sonra yaklaşık 87 g/L olarak bulunmuştur. Ön işleme tabi tutulmamış CC hidrolizinde ise, iyonik olmayan surfektanların (Tween 20 ve Tween 80) indirgeyici şeker verimine fark yaratacak şekilde bir artırma etkisi görülmemiştir.

Anahtar Kelimeler

• enzimatik hidroliz
• şeker pancarı
• mısır koçanı
• istatiksel model
• şeker verimi

INVESTIGATION OF SEVERAL FACTORS ON ENZYMATIC HYDROLYSIS OF SUGAR BEET PULP AND CORN COB: STATISTICAL ANALYSES OF THE EXPERIMENTAL RESULTS

Öz

In this work, sugar beet pulp (SBP) as a lignin poor biomass and corn cob (CC) as a lignin rich biomass were subjected to enzymatic hydrolysis to see the effects of various variables on reducing sugar yield. In SBP hydrolysis, response surface methodology (RSM) and ANOVA were used to fit sugar yield and to determine significance of the parameters (substrate, pectinase, cellulase and hydrolysis time). The proposed quadratic model gave an adequate approximation indicating the significance of all main effects and some of the interaction effects (p < 0.05). The maximum yields within the design space were found approximately as 87 g/L after 18 h of hydrolysis, using 300 µl Cellic Ctec3 and 300 µl Pectinex Ultra SP-L at %20 substrate loading. In CC hydrolysis, the use of nonionic surfactants (Tween 20 and Tween 80) under unpretreated conditions did not necessarily increase the yield of reducing sugar from untreated CC.

Anahtar Kelimeler

• enzymatic hydrolysis
• sugar beet
• corn cob
• statistical modeling
• sugar yield

Kaynakça

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