LISTERIA MONOCYTOGENES SPESİFİK FAJLARIN İZOLASYONU

Yıl 2019, Cilt: 44 Sayı: 3, 463 - 471, 15.05.2019

Pinar Şanlıbaba , Başar Uymaz Tezel

https://doi.org/10.15237/gida.GD19036

Öz

Bu çalışmada Listeria monocytogenes'i enfekte eden
litik fajların izole edilmesi, saflaştırılması ve konakçı etkinliklerinin
belirlenmesi amaçlanmıştır. Toplamda 68 örnek taranmış ve dışkı, gıda işleme
atık suları, balıkçılık atık suları ve balık örneklerinden 4 pozitif izolat
elde edilmiştir. Faj varlığı bakımından taranan örneklerin % 5.88’inde L.
monocytogenes fajı izole edilmiştir. Faj konakçı etkinliğinin belirlenmesi
amacıyla, çift tabaka agar yöntemi kullanılmıştır.  11 L. monocytogenes suşu denenen dört
faja karşı dirençli bulunurken, 24 suşta ise duyarlılık saptanmıştır.  Dört fajın 24 L. monocytogenes suşuna
karşı oluşturduğu faj plak çapı ise 0.4 ve 2.6 mm arasında değişmiştir. Hiçbir
faj birbiri ile aynı konakçı etkinliği göstermemiştir. 

Anahtar Kelimeler

Listeria monocytogenes, Faj, İzolasyon, Konakçı Etkinliği, Duyarlılık

Proje Numarası

15B0443010

ISOLATION OF PHAGES INFECTING LISTERIA MONOCYTOGENES

Öz

It was aimed to isolate, purify
and determine host ranges of lytic phages that infect Listeria monocytogenes
in this study. Out of 68 samples screened, 4 positive isolates were recovered
from feces, food processing waste water, fisheries waste water, and fish
samples. Recovery status of the L. monocytogenes phage was found to be
5.88%. To determine host ranges of phages, soft agar overlay plaque assay was
used. While eleven L. monocytogenes strains showed resistant to all four
isolated phages, 24 strains were sensitive. The plaque sizes of the 4 phages
against 24 L. monocytogenes strains ranged from 0.4 and 2.6 mm. None of
the phages had identical host ranges. 

Anahtar Kelimeler

Listeria monocytogenes, Phage, Isolation, Host Range

Destekleyen Kurum

Ankara University Scientific Research Projects Coordination Unit

Proje Numarası

15B0443010

Kaynakça

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