KARADUT ÖZLERİNİN BİYOKİMYASAL BİLEŞİMİ VE TAĞŞİŞİN BELİRLENMESİ

Yıl 2024, Cilt: 49 Sayı: 6, 1038 - 1061, 09.12.2024

Yakup Şirin , Büşra Erdem , Sertan Cengiz , Semih Gürkan , Perihan Gürkan

https://doi.org/10.15237/gida.GD24071

Öz

Karadut, içeriğindeki yüksek miktarda fenolik bileşikler ve C vitamini gibi biyoaktif maddelerin yanı sıra karbohidrat, yağ, lif, mineral gibi besleyici değerlerden dolayı önemlidir. Bu çalışmada ülkemizde satışı yapılan 35 farklı marka karadut özlerinin fiziksel, biyokimyasal ve besinsel özelliklerinin belirlemesi amaçlanmıştır. Yapılan çalışmalar sonucunda ülkemizde satışı yapılan karadut özlerinin bazılarında HMF değerinin çok yüksek olduğu, birçoğunun protein içermediği ve ilave maltoz ve türevlerini içerdiği görülmüştür. Ayrıca bu karadut özlerinin tamamının mineralce fakir olduğu, ilave tatlandırıcı, koruyucu ve sentetik boya içerdiği tespit edilmiştir. Bu karadut özlerinin birçoğunda sentetik boyaya rastlanmış ve karbon 13 değerlerinin de uygun olmadığı görülmüştür. BME-34 ve 35 marka karadut özü incelendiğinde ise yukarıda bahsedilen tüm değerlerin uygun olduğu ve literatürle de benzer sonuçlar verdiği görülmüştür. Sonuç olarak ülkemizde karadut özü olarak satılan ürünlerin hileli bir ürün olduğu tespit edilmiştir.

Anahtar Kelimeler

Karadut, Meyve Özü, Gıda

BIOCHEMICAL COMPOSITION AND DETERMINATION OF ADULTERATION OF BLACK MULBERRY EXTRACTS

Öz

Black mulberry is crucial for its high content of bioactive substances, such as phenolic compounds and vitamin C, as well as nutritional values, such as carbohydrates, fats, fibre and minerals. This study aimed to determine the physical, biochemical and nutritional properties of 35 different brands of black mulberry extracts sold in our country. Consequently of the studies, it was observed that some of the extracts sold in our country had a very high HMF value; most of them contained no protein and additional maltose and derivatives. In addition, all of these black mulberry extracts were low in minerals and contained additional sweeteners, preservatives and synthetic colourings. Synthetic colourings were found in many of these black mulberry extracts and carbon 13 levels were not appropriate. When BME-34 and 35 brands of black mulberry extract were analysed, all of the above values were appropriate, and consistent with previous studies in the literature.

Anahtar Kelimeler

Black Mulberry, Fruit Extract, Food

Kaynakça

• Ahmed, M., Bose, I., Goksen, G., Roy, S. (2023). Himalayan sources of anthocyanins and its multifunctional applications: A review. Foods, 12(11), 2203.
• Ananga, A., Georgiev, V., Ochieng, J., Phills, B., Tsolova, V. (2013). Production of anthocyanins in grape cell cultures: a potential source of raw material for pharmaceutical, food, and cosmetic industries. The Mediterranean genetic code-grapevine and olive, 1(0), 247-287.
• Aybastier, Ö. (2021). Isolation and determination of antioxidant compounds from black mulberry (Morus nigra L.) by chromatographic methods. Journal of Food, 32-41.
• Benzie, I. F., Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry, 239(1), 70-76.
• Can, Z., Yildiz, O., Sahin, H., Turumtay, E. A., Silici, S., Kolayli, S. (2015). An investigation of Turkish honeys: Their physico-chemical properties, antioxidant capacities and phenolic profiles. Food chemistry, 180, 133-141.
• Castañeda-Ovando, A., de Lourdes Pacheco-Hernández, M., Páez-Hernández, M. E., Rodríguez, J. A., Galán-Vidal, C. A. (2009). Chemical studies of anthocyanins: A review. Food chemistry, 113(4), 859-871.
• Cavalcanti, R. N., Santos, D. T., Meireles, M. A. A. (2011). Non-thermal stabilization mechanisms of anthocyanins in model and food systems—An overview. Food research international, 44(2), 499-509. Chen, P. N., Chu, S. C., Chiou, H. L., Kuo, W. H., Chiang, C. L., Hsieh, Y. S. (2006). Mulberry anthocyanins, cyanidin 3-rutinoside and cyanidin 3-glucoside, exhibited an inhibitory effect on the migration and invasion of a human lung cancer cell line. Cancer letters, 235(2), 248-259.
• Cuendet, M., Hostettmann, K., Potterat, O., Dyatmiko, W. (1997). Iridoid glucosides with free radical scavenging properties from Fagraea blumei. Helvetica Chimica Acta, 80(4), 1144-1152.
• Czaja, T., Sobota, A., Szostak, R. (2020). Quantification of ash and moisture in wheat flour by Raman spectroscopy. Foods, 9(3), 280.
• Dalmagro, A. P., Camargo, A., Rodrigues, A. L. S., Zeni, A. L. B. (2019). Involvement of PI3K/Akt/GSK-3β signaling pathway in the antidepressant-like and neuroprotective effects of Morus nigra and its major phenolic, syringic acid. Chemico-biological interactions, 314, 108843.
• Erarslan, Z. B., Karagöz, S., Kültür, Ş. (2021). Comparative morphological and anatomical studies on Morus species (Moraceae) in Turkey. Turkish Journal of Pharmaceutical Sciences, 18(2), 157.
• Ercisli, S. (2004). A short review of the fruit germplasm resources of Turkey. Genetic Resources and Crop Evolution, 51, 419-435.
• Ercisli, S., Orhan, E. (2007). Chemical composition of white (Morus alba), red (Morus rubra) and black (Morus nigra) mulberry fruits. Food chemistry, 103(4), 1380-1384.
• Espín, J. C., Soler-Rivas, C., Wichers, H. J., García-Viguera, C. (2000). Anthocyanin-based natural colorants: a new source of antiradical activity for foodstuff. Journal of Agricultural and Food Chemistry, 48(5), 1588-1592.
• Fukumoto, L. R., Mazza, G. (2000). Assessing antioxidant and prooxidant activities of phenolic compounds. Journal of agricultural and food chemistry, 48(8), 3597-3604.
• Gerasopoulos, D., Stavroulakis, G. (1997). Quality characteristics of four mulberry (Morus sp) cultivars in the area of Chania, Greece. Journal of the Science of Food and Agriculture, 73(2), 261-264.
• Gundogdu, M., Muradoglu, F., Sensoy, R. G., Yilmaz, H. J. S. H. (2011). Determination of fruit chemical properties of Morus nigra L., Morus alba L. and Morus rubra L. by HPLC. Scientia horticulturae, 132, 37-41.
• Guo, F., Guan, R., Sun, X., Zhang, C., Shan, C., Liu, M., Lin, H. (2023). Integrated metabolome and transcriptome analyses of anthocyanin biosynthesis reveal key candidate genes involved in colour variation of Scutellaria baicalensis flowers. BMC Plant Biology, 23(1), 643.
• Güngör, N. (2007). Dut pekmezinin bazı kimyasal ve fiziksel özellikleri ile antioksidan aktivitesi üzerine depolamanın etkisi. Atatürk Üniversitesi Fen Bilimleri Enstitüsü Gıda Mühendisliği Anabilim Dalı Yüksek Lisans Tezi, Erzurum, Türkiye, 84s.
• Işık, S., Çelik, Ş. (2023). Sürülebilir Özellikte Yeni Kahvaltılık Bir Ürün: Dut Pekmezi Kreması. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 26(2), 326-338.
• Kalt, W., McDonald, J. E., Vinqvist-Tymchuk, M. R., Liu, Y., Fillmore, S. A. (2017). Human anthocyanin bioavailability: effect of intake duration and dosing. Food & Function, 8(12), 4563-4569.
• Kaplan, K. (2022). Konvansiyonel ve organik tahin üretimi sırasında bazı fizikokimyasal özelliklerdeki değişimlerin incelenmesi. Tekirdağ Namık Kemal Üniversitesi Fen Bilimleri Üniversitesi Gıda Mühendisliği Anabilim Dalı Yüksek Lisans Tezi, Tekirdağ, Türkiye, 70s.
• Karataşlı, M., Çakmak, B., Özpınar, H. (2016). Investigation of sorbic acid and benzoic acid amount of some food exposed for sale in Istanbul. International Journal of Food Engineering Research, 2(2), 43-58.
• Katsube, T., Imawaka, N., Kawano, Y., Yamazaki, Y., Shiwaku, K., Yamane, Y. (2006). Antioxidant flavonol glycosides in mulberry (Morus alba L.) leaves isolated based on LDL antioxidant activity. Food chemistry, 97(1), 25-31.
• Kostić, E., Arsić, B., MitiĆ, M., DimitrijeviĆ, D., Marinkovic, E. P. (2019). Optimization of the solid-liquid extraction process of phenolic compounds from mulberry fruit. Notulae botanicae horti agrobotanici cluj-napoca, 47(3), 629-633.
• Koyuncu, F., Çetinbas, M., Erdal, İ. (2014). Nutritional constituents of wild-grown black mulberry (Morus nigra L.). Journal of Applied Botany and Food Quality, 87.
• Kutlu, T., Durmaz, G., Ateş, B., Yilmaz, I., Çetin, M. Ş. (2011). Antioxidant properties of different extracts of black mulberry (Morus nigra L.). Turkish Journal of Biology, 35(1), 103-110.
• Liu, S., Zhang, Y. (2024). Antioxidant properties and electrochemical activity of anthocyanins and anthocyanidins in mulberries. Journal of Food Measurement and Characterization, 1-8.
• Mahesh, D. S., Vidhathri, B. S., Vidyashree, D. N., Narayanaswamy, T. K., Subbarayappa, C. T., Muthuraju, R. (2017). Biochemical composition and pharmacological properties of mulberry (Morus spp.)-a review. International Journal of Current Microbiology and Applied Sciences, 6(7), 2207-2217.
• Mattioli, R., Francioso, A., Mosca, L., Silva, P. (2020). Anthocyanins: A comprehensive review of their chemical properties and health effects on cardiovascular and neurodegenerative diseases. Molecules, 25(17), 3809.
• Memete, A. R., Miere, F., Laslo, V., Purcarea, C., Vicas, L., Ganea, M., Vicas, S. I. (2023). An In Vitro Study of the Healing Potential of Black Mulberry (Morus nigra L.) Extract in a Liposomal Formulation. Applied Sciences, 13(2), 1041.
• Naeem, M. Y. (2020). Medicinal potentials and health benefits of black mulberry. Eurasian Journal of Food Science and Technology, 4(1), 1-5.
• Naseri, R., Farzaei, F., Haratipour, P., Nabavi, S. F., Habtemariam, S., Farzaei, M. H., Momtaz, S. (2018). Anthocyanins in the management of metabolic syndrome: A pharmacological and biopharmaceutical review. Frontiers in Pharmacology, 9, 1310.
• Nguyen, L. T. H. (2022). Biological Activities of Paper Mulberry (Broussonetia papyrifera): More than a Skin-Lightening Agent. Cosmetics, 9(6), 112.
• Okatan, V., Çolak, A. M. (2019). Chemical and phytochemicals content of barberry (Berberis vulgaris L.) fruit genotypes from Sivasli district of Usak province of western Turkey. Pakistan Journal of Botany, 51(1), 165-170.
• Özgen, M., Serçe, S., Kaya, C. (2009). Phytochemical and antioxidant properties of anthocyanin-rich Morus nigra and Morus rubra fruits. Scientia horticulturae, 119(3), 275-279.
• Padovan, G. J., Rodrigues, L. P., Leme, I. A., De Jong, D., Marchini, J. S. (2007). Presence of C4 Sugars in Honey Samples Detected by The Carbon Isotope Ratio Measured by IRMS. Eurasian Journal of Analytical Chemistry, 2(3).
• Palonen, P., Weber, C. (2019). Fruit color stability, anthocyanin content, and shelf life were not correlated with ethylene production rate in five primocane raspberry genotypes. Scientia Horticulturae, 247, 9-16.
• Paunović, S. M., Mašković, P., Milinković, M. (2020). Determination of primary metabolites, vitamins and minerals in black mulberry (Morus nigra) berries depending on altitude. Erwerbs-obstbau, 62(3), 355-360.
• Polat, A. A. (2004). Hatay’ın Antakya ilçesinde yetiştirilen bazı dut tiplerinin meyve özelliklerinin belirlenmesi. Bahçe, 33(1).
• Pruteanu, L. L., Bailey, D. S., Grădinaru, A. C., Jäntschi, L. (2023). The biochemistry and effectiveness of antioxidants in food, fruits, and marine algae. Antioxidants, 12(4), 860.
• Rabrenović, B. B., Dimić, E. B., Novaković, M. M., Tešević, V. V., Basić, Z. N. (2014). The most important bioactive components of cold pressed oil from different pumpkin (Cucurbita pepo L.) seeds. LWT-Food Science and Technology, 55(2), 521-527.
• Rodrigues de Queiroz, A., Hines, C., Brown, J., Sahay, S., Vijayan, J., Stone, J. M., Roston, R. L. The effects of exogenously applied antioxidants on plant growth and resilience. Phytochemistry Reviews. 2023; 22(2), 407-447.
• Sarma, A. D., Sharma, R. (1999). Anthocyanin-DNA copigmentation complex: mutual protection against oxidative damage. Phytochemistry, 52(7), 1313-1318.
• Sendri, N., Bhandari, P. (2024). Anthocyanins: a comprehensive review on biosynthesis, structural diversity, and industrial applications. Phytochemistry Reviews, 1-62.
• Serçe, S., Özgen, M., Torun, A. A., Ercişli, S. (2010). Chemical composition, antioxidant activities and total phenolic content of Arbutus andrachne L.(Fam. Ericaceae)(the Greek strawberry tree) fruits from Turkey. Journal of Food Composition and Analysis, 23(6), 619-623.
• Shekarabi, S. P. H., Omidi, A. H., Dawood, M. A., Adel, M., Avazeh, A., Heidari, F. (2020). Effect of Black Mulberry (Morus nigra) Powder on Growth Performance, Biochemical Parameters, Blood Carotenoid Concentration, and Fillet Color of Rainbow Trout. Annals of Animal Science, 20(1), 125-136.
• Skrovankova, S., Ercisli, S., Ozkan, G., Ilhan, G., Sagbas, H. I., Karatas, N., Mlcek, J. (2022). Diversity of phytochemical and antioxidant characteristics of black mulberry (Morus nigra L.) fruits from Turkey. Antioxidants, 11(7), 1339.
• Skrovankova, S., Sumczynski, D., Mlcek, J., Jurikova, T., Sochor, J. (2015). Bioactive compounds and antioxidant activity in different types of berries. International journal of molecular sciences, 16(10), 24673-24706.
• Slinkard, K., Singleton, V. L. (1977). Total phenol analysis: automation and comparison with manual methods. American journal of enology and viticulture, 28(1), 49-55.
• Suh, H. J., Noh, D. O., Kang, C. S., Kim, J. M., Lee, S. W. (2003). Thermal kinetics of color degradation of mulberry fruit extract. Food/Nahrung, 47(2), 132-135.
• Svanberg, L., Malmberg, K., Gustinelli, G., Öhgren, C., Persson, I., Brive, L., Wassén, S. (2019). Effect of anthocyanins on lipid oxidation and microbial spoilage in value-added emulsions with bilberry seed oil, anthocyanins and cold set whey protein hydrogels. Food chemistry, 272, 273-278.
• Toğrul, Ö., Hayoğlu, İ. (2020). As a new kind of isotonic drinks; the possibilities of the production of apple juice enriched by pomegranate, cranberry and black mulberry juice. Harran Journal of Agricultural and Food Science, 24(2), 165-173.
• Vijayan, K., Chauhan, S., Das, N. K., Chakraborti, S. P., Roy, B. N. (1997). Leaf yield component combining abilities in mulberry (Morus spp.). Euphytica, 98(1), 47-52.
• Wang, R. S., Dong, P. H., Shuai, X. X., Chen, M. S. (2022). Evaluation of different black mulberry fruits (Morus nigra L.) based on phenolic compounds and antioxidant activity. Foods, 11(9), 1252.
• Yıldız, M. U., Özcan, M. M., Çalıșır, S., Demir, F., Er, F. (2009). Physico-chemical properties of wild chestnut (Castanea sativa Mill.) fruit grown in Turkey. World Applied Sciences Journal, 6(3), 365-372.
• Zhang, H., Ma, Z. F., Luo, X., Li, X. (2018). Effects of mulberry fruit (Morus alba L.) consumption on health outcomes: A mini-review. Antioxidants, 7(5), 69.