Tavuk Etinden İzole Edilen Salmonella spp. İzolatlarının Gıda İşletmelerinde Kullanılan Sodyum Hipoklorit ve Benzalkonyum Klorit Biyositlerine Karşı Direncinin Belirlenmesi

Yıl 2025, Cilt: 6 Sayı: 2, 315 - 329, 29.11.2025

Seza Arslan , Uğur Yaman Ateş , Fatma Özdemir , Gizem Alıcı , Eda Doğan

https://doi.org/10.63716/guffd.1793839

Öz

Enterobacteriaceae ailesine ait Salmonella gıda kaynaklı patojendir. Tavuk eti yüksek besin değeri nedeniyle Salmonella’nın kontaminasyonu için uygundur. Gıda endüstirisinde patojen kontaminasyonunu önlemek için biyositler kullanılmakta olup zamanla biyositlere karşı direnç gelişebilmektedir. Çalışmamızda, perakende tavuk etinden izole edilip adlandırılmış Salmonella izolatlarının, gıda endüstrisinde kullanılan sodyum hipoklorit ve benzalkonyum klorit biyositlerine karşı duyarlılıklarının tespiti amaçlanmıştır. 20 Salmonella izolatı 24 ve 48 saat süreyle sodyum hipoklorit ve benzalkonyum klorite maruz bırakılmış ve minimum inhibitör konsantrasyonu (MİK) ve minimum bakterisidal konsantrasyonu (MBK) belirlenmiştir. İzolatların benzalkonyum klorite karşı 24 ve 48 saatte MİK ve MBK değerleri 16-64 µg/mL bulunmuştur. Sodyum hipoklorit için 24 ve 48 saatte MİK ve MBK değerleri 625-2500 ppm arasındadır. Benzalkonyum klorit için her iki saat uygulamasında benzer değerler bulunurken, sodyum hipokloritin süreye bağlı MİK ve MBK değerleri azalmıştır. İstatistiksel olarak her iki biyosit için uygulamalar arası fark anlamlı bulunmamıştır. Benzalkonyum kloritin MİK50 16 µg/mL ve MİK90 32 µg/mL iken, sodyum hipokloritin MİK50 ve MİK90 1250 ppm bulunmuştur. Salmonella Enteritidis ve Salmonella Typhimurium referans suşları da aynı şartlarda biyositlere karşı test edilmiştir. Bazı izolatların biyositlere karşı MİK ve MBK değerlerinin referans suşlardan daha yüksek olduğu görülmüştür. Sonuç olarak, patojenlerin biyositlere karşı gelişen direncinin takip edilmesi halk sağlığı ve gıda güvenliği açısından önemlidir.

Anahtar Kelimeler

Salmonella spp. , Tavuk eti , Sodyum hipoklorit , Benzalkonyum klorit , Minimum inhibitör konsantrasyonu , Minimum bakterisidal konsantrasyonu

Destekleyen Kurum

Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK)

Proje Numarası

1919B012402684 Numaralı Proje

Teşekkür

Bu araştırma, Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK) 2209-A Üniversite Öğrencileri Araştırma Projeleri Destekleme Programı 2024 yılı 1. döneminde 1919B012402684 numaralı proje ile desteklenmiştir. Projemize desteklerinden dolayı TÜBİTAK’a teşekkür ederiz. Ayrıca, çalışmanın gerçekleşmesine destek veren BAİBÜ Fen-Edebiyat Fakültesi, Uygulamalı Mikrobiyoloji Araştırma Laboratuvarına ve Yüksek Lisans öğrencileri Melek Melisa ÖZGÖK ve Şerife Topçuoğlu’na teşekkür ederiz.

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