Investigation of Amounts of Glutathione and Vitamins with Total Antioxidant Capacity in Leaves of Plant Tragopogon reticulatus
Abstract
Interest in native plants is increasing every day. Therefore, it was aimed to determine the amounts of vitamins, beta-carotene, glutathione and the total antioxidant capacity in forage plants (Tragopogon reticulatus) comsumed as either raw or cooked foods. In this study the amounts of reduced glutathione (GSH), oxidized glutathione (GSSG) with vitamin A, vitamin E, Beta-carotene, vitamin C, thiamine hydrochloride (vitamin B1), riboflavin (vitamin B2), nicotinic acid (vitamin B3), pyridoxine hydrochloride (vitamin B6) and folic acid (vitamin B9) in leaves of Tragopogon reticulatus sample were determined by High Performance Liquid Chromatography. The total amounts of the phenolic and flavonoid contents in leaves of plant samples were determined by spectrophotometric methods. In addition, the free radical scavenging effect of the plant extract was measured by using 2,2-diphenyl-1-pikrilhidrazil (DPPH) and this effect was compared with the of butylated hydroxytoluene (BHT) known as standard antioxidant. While the amounts of GSSG, vitamins B3, B6 and C in the leaves of Tragopogon reticulatus sample were found to be high, the amounts of GSH, β-carotene, vitamins A, E, B1, B2 and B9 were low. Free radical scavenging effect was determined as 46.56 ± 0.79 (%) and there was a significant difference (p<0.05) compared to BHT. As a result, the Tragopogon reticulatus plant leaves have rich source of glutathione (GSH, GSSG), β-carotene, vitamins C, B2, B3 and B6 phenolic and flavonoid contents therefore we can said that it has a strong antioxidant effects.
Keywords
Tragopogon reticulatus; Glutathione; Vitamins and Total Antioxidant Capacity
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