Inhibition Kinetics of Sesamol Against Lactoperoxidase System

Yıl 2020, Cilt: 9 Sayı: 4, 389 - 395, 02.01.2021

Ramazan Kalın

https://doi.org/10.37989/gumussagbil.790529

Cited By: 2

Öz

Rich in bioactive components such as antioxidant systems, milk is one of the indispensable products of a healthy diet. It has a natural defense system against harmful micro-organisms. This system is especially important for the growth and development periods of babies. Lactoperoxidase (LPO; EC 1.11.1.7), which is in the oxidoreductase enzyme class, is a member of the peroxidase family commonly found in humans and animals. Sesamol, a compound derived from sesame seeds, is a molecule widely studied by the scientists for the treatment of various diseases and disorders. In this study, in vitro inhibition parameters of sesamol on bovine lactoperoxidase system were determined. The LPO enzyme was purified 467.51 fold with a yield of 75.99% in one step with an affinity chromatography technique using sulfanilamide as ligand. It was determined that sesamol inhibits LPO enzyme strongly at nM level. IC50 and Ki values for Sesamol were calculated as 138.629 and 80.599±3.669, respectively. Also, sesamol showed a competitive inhibition effect on LPO.

Anahtar Kelimeler

Affinity chromatography, In vitro, Lactoperoxidase, Sesamol

Laktoperoksidaz Sistemine Karşı Sesamolün İnhibisyon Kinetiği

Öz

Antioksidan sistemleri gibi biyoaktif bileşenlerce zengin olan süt, sağlıklı bir beslenmenin vazgeçilmez ürünlerinden bir tanesidir. Süt, zararlı mikro-organizmalara karşı doğal bir savunma sistemine sahiptir. Bu sistem, özellikle bebeklerin büyüme ve gelişme dönemleri için çok önemlidir. Oksidoredüktaz enzim sınıfında olan laktoperoksidaz (LPO; EC 1.11.1.7), doğada yaygın olarak insanlarda ve hayvanlarda bulunan peroksidaz ailesinin bir üyesidir. Susam tohumlarından elde edilen bir bileşik olan sesamol, çeşitli hastalık ve bozuklukların tedavisi için bilim camiası tarafından geniş çapta çalışılmış bir moleküldür. Bu çalışmada, sığır laktoperoksidaz sistemi üzerine sesamolün in vitro inhibisyon parametreleri belirlenmiştir. LPO enzimi, ligant olarak sülfanilamitin kullanıldığı bir afinite kromatografisi tekniği ile tek kademede %75,99 verim ile 467,51 kat saflaştırıldı. Sesamol LPO enzimini nM’lar seviyede güçlü bir şekilde inhibe ettiği belirlendi. Sesamol için IC50 ve Ki değerleri sırasıyla 138,629 ve 80,599±3,669 olarak hesaplandı. Aynı zamanda sesamol LPO üzerine yarışmalı bir inhibisyon etkisi gösterdi.

Anahtar Kelimeler

Afinite kromatografisi, İn vitro, Laktoperoksidaz, Sesamol

Kaynakça

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