ANTIBACTERIAL CHARACTERISTICS OF NANOFIBER STRUCTURES OBTAINED BY BENZALKONIUM CHLORIDE ADDITIVE POLY (VINYL ALCOHOL)/GELATIN

Yıl 2019, Cilt: 31 - Özel Sayı II:, 122 - 127, 20.12.2019

Metin Yüksek , Onur Yolay Erdem Tezcan Erkan İşgören Derya Saltık Fatmagül Çalışkan

https://doi.org/10.7240/jeps.608954

Cited By: 1

Öz

Polyvinyl
alcohol (PVA) is a hydrophilous and semi-crystallized. It has attracted much
relevancy due to its pretty chemical endurance, fine thermal determination,
decent physical specialities, wonderful bio-compatibility and cheapness.
Gelatin (G) is a natural polymer and that are interesting materials for
biomedical applications. Electrospinning is a simple method that provides very
porous nanofiber production with high surface area. It is possible to produce
biomedical, filtration, energy storage and protective materials by using
electrospinning method. Benzalkonium chloride (BAC) is a kind of anti-microbial
cationic surface-active agent, which has been pretty used in merchant wound
dressings and has a powerful status toward Gr+ bacteria.

In this study, nanofibers were
produced from electrospinning of BAC, PVA and G containing solutions at various
concentrations. The fibers of obtained nanofiber structure were uniform,
continuous and intensive. The optimum parameters in terms of good mechanical
and antibacterial properties were determined. S2 sample, electrospun from 11.63% PVA + 1.0% G +1.0% BAC
containing solution, had the best morphological and mechanical properties due
to having the thinnest fiber diameter (51±13nm) and the highest vertical
strength (4.299MPa) and horizontal strength (4.058MPa). It also had antibacterial
activity against all the bacteria tested (E. coli,
P. aeruginosa, B. subtilis and S. aureus). Due to owning
good mechanical and antibacterial properties, S2 sample can have many uses in
medical sector.

Anahtar Kelimeler

Electrospinning, poly(vinyl alcohol), gelatin, benzalkonium chloride, antibacterial, nanofiber

Benzalkonyum Klorür Katkılı PVA/Gelatin Nanolif Yapılarının Antibakteriyel Özellikleri

Öz

Poli (vinil alkol) (PVA), iyi kimyasal dayanımı, iyi termal kararlılığı, iyi fiziksel özellikleri, mükemmel biyouyumluluğu ve ucuzluğu nedeniyle çok dikkat çeken hidrofilik, yarı kristalli bir polimerdir. Jelatin (G) biyomedikal uygulamalar için ilginç ve doğal bir polimerdir. Elektroeğirme, yüksek yüzey alanına sahip çok gözenekli nano elyaf üretimi sağlayan basit bir yöntemdir. Elektrospinning yöntemi kullanılarak biyomedikal, filtrasyon, enerji depolama ve koruyucu malzemeler üretmek mümkündür. Benzalkonyum klorür (BAC), ticari yara sargısında yaygın olarak kullanılan ve Gram-pozitif bakterilere karşı güçlü bir role sahip olan bir tür antimikrobiyal katyonik yüzey aktif malzemedir. Bu çalışmada, çeşitli konsantrasyonlarda BAC, PVA ve G içeren çözeltilerin nano lifler üretilmiştir.  Elde edilen nano lif yapısının lifleri homojen, sürekli ve yoğundur. İyi mekanik ve antibakteriyel özellikler açısından optimum parametreler belirlenmiştir. 11.63% PVA + 1.0% G +1.0% BAC içeren S2 örneği en iyi morfolojik ve mekanik özellikler göstermesinin yanında en ince elyaf çapına (51 ± 13nm) ve en yüksek dikey dayanıma (4.299MPa) ve yatay dayanıma (4.058MPa) sahiptir.  Ayrıca test edilen tüm bakterilere (E. coli, P. aeruginosa, B. subtilis ve S. aureus) karşı antibakteriyel aktiviteye sahiptir. İyi mekanik ve antibakteriyel özelliklere sahip olması nedeniyle, S2 örneğinin tıbbi sektörde birçok kullanımı olabileceği sonucuna ulaşılmıştır.

Anahtar Kelimeler

Elektroeğirme, PolivinilAlkol, PVA, Benzalkonyum Klorür, BAC, Antibakteriyel, Nanolif

Kaynakça

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