HYPERICUM SCABRUM L. EKSTRESİNİN ANTİOKSİDAN, ENZİM İNHİBİTÖR AKTİVİTESİNİN DEĞERLENDİRİLMESİ VE YPSK ANALİZİ

Öz

Amaç: Diyabet ve obezite günümüzün en yaygın sağlık problemleri arasında yer almaktadır. Bu problemlerin tedavisi için yeni ilaç kaynaklarına ihtiyaç duyulmaktadır. Toplumda geleneksel olarak kullanımı ile bilinen Hypericum scabrum L. bitkisi bu araştırmanın konusu olmuştur. Araştırma kapsamında H. scabrum bitkisinin toprak üstü kısımlarından hazırlanan etanol (%80) ekstresinin, antioksidan, antidiyabetik, anti-obezite, antikolesterol aktiviteleri ile fitokimyasal içeriğinin incelenmesi amaçlanmıştır.

Gereç ve Yöntem: H. scabrum bitkisinin toprak üstü kısımlarından hazırlanan etanol (%80) ekstresinin toplam fenol, toplam flavonoit miktar tayini yapılmış ve fitokimyasal içerik değerlendirilmiştir. Aynı zamanda ekstre içeriğinde bulunan fenolik ve flavonoit bileşenler ters faz Yüksek Performanslı Sıvı Kromatografisi YPSK ile araştırılarak miktar tayini gerçekleştirilmiştir. Ekstrenin antioksidan etkisi DPPH radikal süpürücü etki, metal bağlama kapasite, demir indirgeme gücü, ABTS radikal süpürücü etki, toplam antioksidan kapasite testleriyle değerlendirilmiştir. Ekstrenin antidiyabetik etkisinin araştırılmasında a-glukozidaz ve a-amilaz enzim inhibitör aktiviteleri incelenmiştir. Anti-obezite, lipit metobolizma üzerinde etkilerinin araştırılmasında sırasıyla pankreatik lipaz ve kolesterol esteraz enzim inhibitör aktiviteleri incelenmiştir.

Sonuç ve Tartışma: Ekstre 2 mg/ml konsantasyonda %90.16 ± 0.13 DPPH radikal süpürücü etki ve %97.53 ± 0.22 ABTS radikal süpürücü etki göstermiştir. Ekstre 2 mg/ml konsantrasyonda a-glukozidaz enzimi üzerinde %98.80 ± 0.51 inhibitör etki göstermiş olup, bu değer standart madde olarak kullanılan akarboza (%99.13 ± 0.11) yakın bulunmuştur. Ekstre 2 mg/ml konsantrasyonda kolesterol esteraz enzimi üzerinde %68.63 ± 1.05 inhibitör etki göstermiştir. Ters faz YPSK analiz sonucuna göre ekstrenin 1.047 ± 0.000 g/100 g ekstre klorojenik asit içerdiği bulunmuştur. Bu bulgulara göre ekstrenin antidiyabetik etkili doğal bir kaynak olabileceği ve bu etkinin incelenmesi için daha ileri çalışmalara ihtiyaç duyulduğu sonucuna varılmıştır.

Anahtar Kelimeler

• Antidiyabetik
• antihiperlipidemik
• antioksidan
• anti-obezite
• Hypericum scabrum

EVALUATION OF ANTIOXIDANT, ENZYME INHIBITOR ACTIVITY OF HYPERICUM SCABRUM EXTRACT AND HPLC ANALYSIS

Öz

Objective: Diabetes and obesity are among the most common health problems today. New drug sources are needed to treat these problems. Hypericum scabrum L. , which is known for its traditional use among the public, has been the subject of this research. Within the scope of the research, it was aimed to investigate the antioxidant, antidiabetic, anti-obesity, anticholesterol activities, and phytochemical content of ethanol (80%) extract prepared from the aerial parts of the H. scabrum.

Material and Method: The total phenol, total flavonoid contents, and phytochemical content of the ethanol (80%) extract prepared from the aerial parts of the H. scabrum were evaluated. Additionally, the phenolic and flavonoid compounds in the extract were investigated with reverse phase HPLC and their quantitative analysis was carried out. The antioxidant effect of the extract was evaluated with DPPH radical-scavenging effect, metal chelating capacity, ferric reducing power, ABTS radical scavenging effect, and total antioxidant capacity tests. In the investigation of the antidiabetic effect of the extract, α-glucosidase and α-amylase enzyme inhibitory activities were investigated. To investigate the effects of anti-obesity and lipid metabolism, pancreatic lipase enzyme inhibitory activity and cholesterol esterase enzyme inhibitory activity were investigated, respectively.

Result and Discussion: The extract showed 90.16 ± 0.13% DPPH radical scavenging and 97.53 ± 0.22% ABTS radical scavenging activity at 2 mg/ml concentration. The extract had an inhibitory activity of 98.80 ± 0.51 % on the α-glucosidase enzyme at a concentration of 2 mg/ml, and this value was close to the acarbose result used as the standard substance (99.13 ± 0.11%). The extract showed inhibitory activity of 68.63 ± 1.05% on the cholesterol esterase enzyme at a concentration of 2 mg/ml. According to the results of reversed-phase HPLC analysis, it was found that the extract contained 1.047 ± 0.000 g/100 g extract chlorogenic acid. According to the results, it was concluded that the extract may be a natural source with antidiabetic effects and further studies are needed to investigate this effect.

Anahtar Kelimeler

• Antidiabetic
• antihyperlipidemic
• antioxidant
• anti-obesity
• Hypericum scabrum

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