AROCLOR 1254’ÜN SIÇAN KALBİ VE TİROİT ÜZERİNDEKİ TOKSİK ETKİLERİ VE SELENYUM DÜZEYLERİNİN DEĞİŞTİRİCİ ROLÜ

Öz

Amaç: Poliklorlu bifeniller (PCB'ler) geçmişte endüstriyel ürünlerde çok yaygın olarak kullanılmıştır. Bu kimyasallar, toksik etkileri nedeniyle 1970'lerde yasaklanmıştır. PCB'ler, çevrede kalıcı oldukları için insan sağlığını hala etkileyebilir. Aroclor 1254 (A1254), elektrik transformatörlerinde, fluoresan aydınlatma armatürlerinde ve televizyon veya buzdolabı gibi eski cihazlarda kullanılan ticari bir PCB türevidir. Bu çalışmada erkek Sprague-Dawley sıçanlarında A1254'ün kalp ve tiroid üzerindeki toksik etkilerinin değerlendirmesi amaçlanmıştır. Ayrıca selenyum durumunun düzenleyici rolü de değerlendirilmiştir. Gereç ve Yöntem: Çalışmada 8 haftalık erkek Sprague-Dawley (SD) ratlar kullanılmıştır. Hayvanlar, kontrol grubu, selenyum suplemente grup (SeS); selenyum eksikliği olan grup (SeD); A1254 maruziyet grubu (A); selenyum suplemente A1254'e maruziyet grubu (ASeS) ve selenyum eksikliği olan A1254'e maruziyet (ASeD) olarak rastgele 6 gruba ayrıldı. A1254, beslenme sürecinin son 15 gününde uygulanmıştır. Kalp ve tiroid ağırlıkları ve bağıl ağırlıkları, plazma tiroid hormonu seviyeleri ve ayrıca tiroid ve kalp dokusu oksidatif/antioksidatif parametreleri değerlendirilmiştir. Sonuç ve Tartışma: Sonuçlar, A1254 maruziyetinin ve selenyum eksikliğinin hem kalp hem de tiroit dokusunda oksidatif strese neden olduğunu göstermiştir. Plazma fT3 ve fT4 seviyeleri ASeD grubunda belirgin şekilde değişmştir. Sonuç olarak, A1254 maruziyetinin hem tiroit hem de kalpte oksidan/antioksidan dengesizliğe yol açabileceği ve tiroit hormonlarının işleyişini bozabileceği söylenebilir. Selenyum, her iki organda da A1254 toksisitesinde değiştirici bir role sahip olduğu görünmektedir ve bunun önemi daha ileri mekanistik deneylerle değerlendirilmelidir.

Anahtar Kelimeler

• Aroklor 1254
• kardiyotoksisite
• oksidatif stress
• selenyum
• tiroit bozuklukları

TOXIC EFFECTS OF AROCLOR 1254 ON RAT HEART AND THYROID AND MODIFYING ROLE OF SELENIUM STATUS

Öz

Objective: Polychlorinated biphenyls (PCBs) were very widely used in industrial products in past. These chemicals were banned in the 1970s due to their toxic effects. PCBs can still affect human health, as they are persistent in the environment. Aroclor 1254 (A1254) is a commercial PCB congener which was used in electrical transformers, fluorescent lighting fixtures and old appliances such as televisions or refrigerators. In this study, we aimed to evaluate the toxic effects of A1254 on heart and thyroid in male Sprague-Dawley rats. In addition, the modifying role of selenium status was also evaluated. Material and Method: 8-week-old male Sprague-Dawley (SD) rats were used in the experiment. The animals were separated randomly into 6 groups (n=6) as control; selenium supplemented (SeS); selenium deficient (SeD); A1254 exposed (A); selenium supplemented A1254 exposed (ASeS) and selenium deficient A1254 exposed (ASeD). A1254 was applied by gavage during the last 15 days of feeding period. Heart and thyroid weights and relative weights, plasma thyroid hormone levels, as well as thyroid and heart tissue oxidative/antioxidative parameters were evaluated. Result and Discussion: Results showed that A1254 exposure and selenium deficiency caused oxidative stress on both heart and thyroid. Plasma fT3 and fT4 levels markedly changed in ASeD group. In conclusion, it can be stated that A1254 exposure can cause lead to oxidative/antioxidative imbalance in both thyroid and heart and can disrupt functioning of thyroid hormones. Selenium seems to have a modifying role in A1254 toxicity in both organs, the importance of which should be evaluated with further mechanistic experiments.

Anahtar Kelimeler

• Aroclor 1254
• cardiotoxicity
• oxidative stress
• selenium
• thyroid disorders

Kaynakça

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