INTERACTION BETWEEN ELTROMBOPAG AND HUMAN SERUM ALBUMIN: FLUORESCENCE AND ELECTROCHEMICAL STUDIES

Öz

Objective: This research investigated the interaction between Eltrombopag (EB) and Human Serum Albumin (HSA) by fluorescence spectroscopy and voltammetry while explaining the quenching mechanism. Material and Method: The electrochemical studies were conducted in an acetate buffer solution (AB) at pH 4 by differential pulse voltammetry (DPV). For the fluorescence studies, EB, HSA, and a mixture of EB and HSA solutions were designed with a pH of 4.7, AB containing 20% DMSO. Result and Discussion: The interaction between EB and HSA was examined by fluorescence and electrochemical titrations, which showed a quenching effect and peak shifting. Fluorescence titrations indicate that EB’s HSA quenching process is static and has a hypsochromic shift. Analysis of thermodynamic parameters and higher binding constants concluded a strong and spontaneous interaction. Electrochemical titrations show the intercalation of EB into HSA.

Anahtar Kelimeler

• Differential pulse voltammetry
• drug protein interaction
• eltrombopag
• fluorescence spectroscopy
• human serum albumin

ELTROMBOPAG VE İNSAN SERUM ALBÜMİNİ ARASINDAKİ ETKİLEŞİM: FLORESANS VE ELEKTROKİMYASAL ÇALIŞMALAR

Öz

Amaç: Bu araştırmada Eltrombopag (EB) ile İnsan Serum Albümini (HSA) arasındaki etkileşim floresan spektroskopisi ve voltametri ile incelenerek sönümlenme mekanizması açıklanmıştır. Gereç ve Yöntem: Elektrokimyasal çalışmalar, pH 4'te asetat tampon çözeltisinde (AB) diferansiyel darbe voltametrisi (DPV) ile gerçekleştirildi. Floresan çalışmaları için EB, HSA ve EB ve HSA çözeltilerinin karışımı, %20 DMSO içeren pH 4.7 AB ile tasarlandı. Sonuç ve Tartışma: EB ve HSA arasındaki etkileşim floresan ve elektrokimyasal titrasyonlar ile incelendi ve sönümlenme etkisi ile pik kayması elde edildi. Floresan titrasyonları, EB'nin HSA sönümleme işleminin statik olduğunu ve hipsokromik bir kayma olduğunu gösterir. Termodinamik parametrelerin ve daha yüksek bağlanma sabitlerinin analizi güçlü ve kendiliğinden bir etkileşimin olduğunu ortaya koydu. Elektrokimyasal titrasyonlar, EB'nin HSA'ya interkalasyonunu gösterir.

Anahtar Kelimeler

• Diferansiyel darbe voltametrisi
• eltrombopag
• floresan spektroskopisi
• ilaç protein etkileşimi
• insan serum albümini

Kaynakça

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