SUILLUS LUTEUS (L).ROUSSEL EKSTRESİ’NİN ANTİOKSİDAN, ANTİMİKROBİYAL VE ANTİ-PROLİFERATİF AKTİVİTESİ

Öz

Amaç: Birçok ilaç keşfinde doğa, doğal ürünler benzeri bileşik sınıflarının tasarımına ilham kaynağı olarak kullanmıştır. Eski zamanlardan beri yenilebilir mantarlar hem gıda hem de ilaç olarak kullanılmıştır. Türkiye'de yaşayan insanlar Suillus luteus (L.) Roussel yabani yenilebilir mantarlarını yaygın olarak tüketmektedir. Bu çalışmada, çeşitli S.luteus ekstraktlarının antioksidan, antimikrobiyal ve anti-proliferatif aktiviteleri araştırıldı.

Gereç ve Yöntem: S.luteus'un antioksidan aktivitesi, DPHH serbest radikal süpürme yöntemi ve demir iyonu şelatlama kabiliyeti saptandı. Ek olarak, toplam fenoller, β-karoten ve likopen gibi antioksidan özelliklere sahip bileşenlerin konsantrasyonu spektrofotometrik yöntemlerle belirlenmiştir. Antimikrobiyal potansiyel, 14 mikroorganizma üzerinde agar difüzyon yöntemi ile gösterilmiştir. Son olarak, S. luteus metanol ekstresinin MCF-7 kanser hücre hatları üzerindeki sitotoksik etkisi MTT yöntemi kullanılarak değerlendirildi.

Sonuç ve Tartışma: Sonuçlar S.luteus metanolik ve etanolik özütlerin daha bol fenollere sahip olduğunu gösterdi. (sırasıyla 153, 49.33 mg GAE/g ekstre,) Ayrıca β-karoten ve likopen içeriği saptandı (0.120 ile 0.606 µg/mL arası) S. luteus’un metanol ve etanol özütlerinin, DPPH radikaline karşı antioksidan aktiviteleri aynı konsantrasyondaki standart antioksidanlar olan BHT’den daha yüksek aktivite göstermiştir (Metanol özütü (IC50: 63.72µg/mL) > Etanol özütü (IC50: 80.72 µg/mL) > BHT (IC50: 96.47µg/mL). Ek olarak, metanol ektresinin etanol ekstresine göre daha yüksek demir iyonu şelatlama kabiliyetine sahiptir (sırasıyla 2.72, 3.45 µg/mL). Genel olarak, test edilen mantar ekstreleri test edilen mikroorganizmalara karşı nispeten düşük antimikrobiyal aktiviteye sahiptir (9 ve 10mm zonçapı) Ayrıca, S. luteus’un metanol ekstresinin 1mg/mL konsantrasyonda kanser hücrelerinin tamamını öldürdüğü tespit edildi. Bu çalışmanın sonuçları incelendiğinde S.luteus'un özellikle metanol ekstresinin potansiyel medikal özelliklere sahip olduğu gösterilmiştir.

Anahtar Kelimeler

• antimikrobial aktivite,antioksidan aktivite,antiproliferatif aktivite,Suillus luteus

ANTIOXIDANT, ANTIMICROBIAL AND ANTI-PROLIFERATIVE ACTIVITY OF SUILLUS LUTEUS (L.) ROUSSEL EXTRACTS

Öz

Objective: Many drug discovery have used nature as an inspiration for the design of naturel products like compound classes. From ancient times edible mushrooms have been used both as food and medicine. People living in Turkey widely consume Suillus luteus (L.) Roussel wild edible mushrooms In this study, we were investigated antioxidant, antimicrobial and cytotoxic activities of various extracts of S.luteus.

Material and Method: Antioxidant activity of S.luteus was detected method by DPHH free radical scavenging and ferrous ion chelating ability. In addition, the concent of the components with antioxidant properties, such as total phenols,β-caratone and lycopene were determined by spectrophotometric methods. The antimicrobial potential was demonstrated with a agar well diffusion method on 14 microorganisms. Fınally, the cytotoxic effect of methanolic extract of S. luteus on MCF-7 cancer cell lines were evaluated by using MTT method.

Result and Discussion: The results indicated that S.luteus methanolic and ethanolic extracts have more abundant phenols (153, 49.33 mg GAE/g extract, respectively).In addıtıon β-caratone and lycopene content detected. (from 0.120 to 0.606µg/mL).S.luteus extracts had more potent free radical scavenging activity than standard antioxidants BHT. (Methanol extract (IC50: 63.72µg/mL) > Ethanol extract (IC50: 80.72 µg/mL) > BHT (IC50: 96.47µg/mL). In addition, methanol extracts possessed higher ferrous ion chelating ability than ethanol extracts(2.72, 3.45 µg/mL, respectively) .Generally, the tested mushroom extracts had relatively low antimicrobial activity against the tested microorganisms (9 and 10 mm zone diameter). Also, S.luteus methanolic extract was found to kill all cancer cells at a concentration of 1mg/mL. These results showed that S.luteus, especially methanol extracts, have potential medical.

Anahtar Kelimeler

• antimicrobial activity,antioxidant activity,anti-proliferative activity,Suillus luteus

Kaynakça

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