Investigating In Vitro Antioxidant and Antimicrobial Activity of Different Sorbus Species in Artvin Province of Türkiye

Öz

In the present study, three Sorbus species in the Rosaceae family naturally growing in Artvin province of Turkey were collected. To determine the antioxidant activity, total phenolic and flavonoids capacity of the extracts, their scavenging capacity for (2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, reducing capacity for Fe3+ (FRAP) and copper (II) ions (CUPRAC) were analyzed. Besides, disc diffusion method was used to determine antibacterial activity. It was found that all Sorbus fruit, flower, leaf and pedicle methanolic extracts showed different levels of antioxidant activity. Results of the the total polyphenol, total flavonoid, FRAP, CUPRAC and DPPH analysis, the highest activity was measured in S. persica pedicle, S. umbellate var. cretica leaf, S. persica leaf, S. umbellata var. cretica leaf and S. persica leaf extracts as 25.7 ± 16.49 mg GAE/g, 7.469 ± 0.4926 mg of quercetin/g, 6.248 ± 0.2374 μmol FeSO4.7H2O/g and, 164.4 ± 4.209 mmol TEAC and 46.33 μg/mL, respectively. It was revealed that methanolic extracts of Sorbus plant showing antibacterial activity had very high minimum inhibitory concentration (MIC) values compared to ampicillin. Thus, considering the findings of the present study, it could be stated that these species merit further studies as natural antioxidant and antibacterial sources.

Anahtar Kelimeler

• Antimicrobial
• Antioxidant
• Methanolic
• extraction
• Sorbus

Kaynakça

1. Akbar, A. & Anal, K. A. (2011). Food safety concerns and food-borne pathogens, Salmonella, Escherichia coli and Campylobacter. FUUAST Journal of Biology, 5, 5-17.
2. Aladedunye, F.  Matthäus, B. (2014). Phenolic extracts from Sorbus aucuparia (L.) and Malus baccata (L.) berries: Antioxidant activity and performance in rapeseed oil during frying and storage. Food Chemistry, 159, 273-28.
3. Apak, R., Güçlü, K., Özyürek, M. & Karademir, E.S. (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins c and e, using their cupric ion reducing capability in the presence of neocuproine: cuprac method. Journal of Agricultural and Food Chemistry, 52, 7970–7981.
4. Arts, C.W.I & Hollman, C.H.P. (2005). Polyphenols and disease risk in epidemiologic studieS. The American Journal of Clinical Nutrition, 8, 317-325.
5. Benzie, J.F. & Strain, J.J. (1999). Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology, 299, 15-27.
6. Berna, E., Kampuse, S., Dukalska, L. & Murniece, I. (2001). The chemıcal and physıcal propertıes of sweet rowanberrıes ın powder sugar. Foodbalt.
7. Bobinaitė, R., Grootaert, C., Camp, V.J. & Sarkinas, A. (2020). Chemical composition, antioxidant, antimicrobial and antiproliferative activities of the extracts isolated from the pomace of rowanberry (Sorbus aucuparia L.). International Food Research Journal, 136, 109310. https://doi.org/10.1016/j.foodreS.2020.109310
8. Bouayed, J. (2010). Double-edged swords in cellular redox state. Oxidative Medicine and Cellular Longevity, 3(4), 228-237. https://doi.org/10.4161/oxim.3.4.12858

9. Chang, C. C., Yang, M.H., Wen, H.M. & Chern, J.C. (2002). Estimation of total flavonoid content in propolis by two complementary. Journal of Food and Drug Analysis, 10 (3), 178-182.
10. Cheesman, J.M., Ilanko, A., Blonk, B. & Cock, E.I. (2017). Developing new antimicrobial therapies: are synergistic combinations of plant extracts/compounds with conventional antibiotics the solution? Pharmacognosy Research, 11, 57-60.
11. Chuah, L.E., Zakaria, A.Z., Suhail, Z., Bakar, A.S. & Desa, M.N.M. (2014). Antimicrobialactivities of plant extracts against methicillin-susceptible and methicillin-resistant StaphylococcusaureuS. Journal of Microbiology Research, 4, 6-13. https://doi.org/ 10.5923/j.microbiology.20140401.02
12. Cook, C.N. & Samman, S. (1996). Flavonoids-chemistry, metabolism, cardioprotective effects, and dietary sourceS. The Journal of Nutritional Biochemistry, 7, 66-76.
13. Çoban, F., Tosun, M., Özera, H., Güneş, A., Öztürka, E., Atsan, E. & Polat, T. (2021). Antioxidant activity and mineral nutrient composition of Polygonum cognatum – a potential wild edible plant. Indian Journal of Traditional Knowledge, 20 (1), 221-229.
14. Davis, H.P., Cullen, J. & Coode, E. J. M. (1965). Flora of Turkey and The East Aegean IslandS. Edinburgh University Press, Edinburgh.
15. Hasbal, G., Yılmaz-Ozden, T. & Can, A. (2015). Antioxidant and antiacetylcholin esterase activities of Sorbus torminalis (L.) Crantz (wild service tree) fruitS. Journal of Food and Drug Analysis, 23, 57-62.
16. Huang, W.Z., Dai, X.J., Liu, Y.Q., Zhang, C.F., Zhang, M. & Wang, Z.T. (2006). Studies on antibacterial activity of flavonoids and diaryl heptanoids from Alpinia Katsumadai. Journal of Plant Resources and Environment, 15, 37–40.
17. Hukkanen, A.T., Pölönen, S.S., Kärenlampı, S.O. & Kokko, H.I. (2006). Antioxidant capacity and phenolic content of sweet rowan berrieS. Journal of Agricultural and Food Chemistry, 54, 112-119.
18. Kavak, D.D. & Akdeniz, B. (2019). Sorbus umbellata (Desf.) Fritsch var. umbellata leaves: optimization of extraction conditions and investigation antimicrobialcytotoxic, and β-glucuronidase inhibitory potential. Plant Foods for Human Nutrition, 74, 364–369. https://doi.org/10.1007/s11130-019-00743-9
19. Korkut, S., Guller, B., Aytin, A. & Kök, S.A. (2009). Turkey’s native wood species: physical and mechanical characterization and surface roughness of rowan (Sorbus Aucuparıa L.). Wood Research, 54, 19-30.
20. Kylli, P., Nohynek, L., Puupponen-Pimia, R., Westerlund-Wikstrom, B., Mcdougall, G., Stewart, D. & Heinonen, M. (2010). Rowanberry phenolics: compositional analysis and bioactivitieS. Journal of Agricultural and Food Chemistry, 58, 11985–11992. https://doi.org/10.1021/jf102739v
21. Liepiņa, I., Nikolajeva, V. & Jākobsone, I. (2013). Antimicrobial activity of extracts from fruits of Aronia melanocarpa and Sorbus aucuparia. Environmental and Experimental Botany, 11, 195–199.
22. Ndhlala, R.A., Moyo, M. & Staden, V. J. (2010). Natural antioxidants: fascinating or mythical biomolecules? Molecules, 15, 6905-6930. https://doi.org/10.3390/molecules15106905
23. Olszewska, A., Nowak, S., Mıchel, P., Banaszczak, P. & Kıcel, A. (2010). Assessment of the content of phenolics and antioxidant action of inflorescences and leaves of selected speciesfrom the genus Sorbus sensu stricto. Molecules, 15, 8769- 8783. https://doi.org/10.3390/molecules15128769
24. Olszewska, A. M., Presler., A. & Michel, P. (2012). Profiling of phenolic compounds and antioxidant activity of dry extracts from the selected Sorbus specieS. Molecules, 17, 3093-3113. https://doi.org/10.3390/molecules17033093
25. Olszewska, A.M. (2011). In vitro antioxidant activity and total phenolic content of the inflorescences, leaves and fruits of Sorbus Torminalis (L.) Crantz. Acta Poloniae Pharmaceutica, 66, 945-953.
26. Raudonis, R., Raudone, L., Gaivelyte, K., Viskelis, P. & Janulis, V. (2014). Phenolic and antioxidant profiles of rowan (Sorbus L.) fruitS. Natural Product Research. http://doi.org/10.1080/14786419.2014.895727
27. Savoia, D. (2012). Plant-derived antimicrobial compounds: alternatives to antibioticS. Future Microbiology, 7(8), 979–990.
28. Silva, D.M., Costa, A.P., Ribon, O.A., Purgato, A.G., Gaspar, M.D. & Diaz, N. A. M. (2019). Plant Extracts Display Synergism with Different Classes of AntibioticS. Access Anais da Academia Brasileira de Ciências, 91(2). https://doi.org/10.1590/0001-3765201920180117
29. Slinkar, K. & Singleton, V. L. (1977). Total phenol analysis: automation and comparison with manuel methodS. American Journal of Enology and Viticulture, 28, 49-55.
30. Tahirovic, A., Mehic, E., Kjosevski, N. & Basic, N. (2019). Phenolics content and antioxidant activity of three Sorbus specieS. Glasnik Hemičarai Tehnologa Bosnei Hercegovine, 53, 15-21. https://doi.org/10.35666/ghtbh.2019.53.03
31. Turumtay, H., Midilli, A., Akyuz- Turumtay, E., Demir, A., Kılıçkaya-Selvi, E., Budak, E. E., Er, H.,
32. Kocaimamoglu, F., Baykal, H., Beldüz, A. O., Atamov, V. & Sandal, C. (2017). Gram (−) microorganisms DNA polymerase inhibition, antibacterial and chemical properties of fruit and leaf extracts of Sorbus acuparia and Sorbus caucasica var. Yaltirikii. Biomedical Chromatography, 31, e3901. https://doi.org/10.1002/bmc.3901
33. Yu, T., Lee, J. Y., Jang, J.H., Kim, R.A., Hong, S., Kim, T. W., Kim, M. Y., Jaehwi, L., Lee, Y.G., & Cho, J. Y. (2011). Anti-inflammatory activity of Sorbus commixta water extract and its molecular inhibitory mechanism. Journal of Ethnopharmacology, 134, 493–500. http://doi.org/10.1016/j.jep.2010.12.032
34. Zymone, K., Raudone, L., Raudonis, R., Marksa, M., Ivanauskas, L., & Janulis, V. (2018). Phytochemical Profiling of Fruit Powders of Twenty Sorbus L. Cultivars, 23, 2593. http://doi.org/10.3390/molecules2310259.