Effect of Various Pretreatments on Germination of Turkish Endemic Achillea gypsicola Hub.-Mor. Species under In Vivo and In Vitro Conditions

Yıl 2019, , 2321 - 2329, 01.12.2019

Muhammed Akif Açıkgöz , Şevket Metin Kara

https://doi.org/10.21597/jist.511469

Cited By: 2

Öz

There has been an increasing interest to overcome seed dormancy in medicinal and aromatic plants using various pretreatments. This study was carried out to determine effect of several pretreatments on germination of Turkish endemic Achillea gypsicola Hub.-Mor. species under in vivo and in vitro conditions. The seeds were subjected to three pretreatments as various concentrations of GA3 and KNO3 and 91day cold stratification, with a seven-day interval. In vivo germination test produced no germination in the seeds pre-treated with cold stratification and various concentrations of GA3, whereas KNO3 treatments both in vivo and in vitro explicitly increased germination percentage. Cold stratification showed no inciting effect on germination both in vitro and in vivo conditions. In vitro combined application of KNO3 and GA3 produced higher germination percentages than single applications. The stimulating effect of KNO3 and GA3 on germination percentage increased as the duration of seed immersing in KNO3 increased. The seeds hold in 200 μM KNO3 for 48 hours and planted on MS medium supplemented with 2 mg L-1 GA3 showed 100% germination. In conclusion, this study suggested that combined pre-treatment of KNO3 with GA3 had a great potential in breaking seed dormancy and increasing germination of Turkish endemic Achillea gypsicola Hub.-Mor. species.

Anahtar Kelimeler

Cold stratification, dormancy, gibberellic acid, potassium nitrate, seed

Destekleyen Kurum

Ordu University

Proje Numarası

AR-1644

Teşekkür

We are thankful to Scientific Research Projects Unit (BAP) of Ordu University for providing support to this research, with the number of AR-1644 BAP Project.

In Vivo ve In Vitro Koşullar Altında Çeşitli Ön Uygulamaların Türkiye Endemiği Achillea gypsicola Hub.-Mor. Türünün Çimlenmesi Üzerine Etkisi

Öz

Tıbbi ve aromatik bitkilerde tohum dormansisinin üstesinden gelmek için çeşitli ön uygulamalara karşı artan bir ilgi vardır. Bu çalışma, çeşitli ön uygulamaların Türkiye endemiği Achillea gypsicola Hub.-Mor. türünün in vivo ve in vitro koşullarda çimlenmesi üzerine etkisini belirlemek amacıyla yapılmıştır. Tohumlar, GA3 ve KNO3'ün çeşitli konsantrasyonları ve yedi günlük aralıklarla 91 günlük soğukta katlama gibi üç ön muameleye tabi tutulmuştur. In vivo çimlenme testinde, soğukta katlama ve çeşitli GA3 dozları ile ön muamele edilmiş tohumlarda çimlenme görülmezken, hem in vivo hem de in vitro koşullarda KNO3 uygulamaları çimlenme yüzdesini arttırmıştır. Soğuk katlama, hem in vitro hem de in vivo koşullarda çimlenme üzerinde hiçbir teşvik edici etki göstermemiştir. KNO3 ve GA3'ün in vitro koşullarda kombine uygulanması, tek başlarına uygulamalarından daha yüksek çimlenme yüzdeleri üretmiştir. Tohumların KNO3'te bekletme süresi arttıkça KNO3 ve GA3'ün çimlenme yüzdesi üzerindeki uyarıcı etkisi artmıştır. 48 saat boyunca 200 μM KN03 çözeltisinde bekletilen ve 2 mg L-1 GA3 ile takviye edilmiş MS ortamına ekilen tohumlarda, %100 çimlenme görülmüştür. Sonuç olarak, bu çalışma KNO3'ün GA3 ile birleştirilmiş ön uygulamasının tohum dormansisini kırmada ve endemik Achillea gypsicola Hub.-Mor. türünün çimlenmesini artırmada büyük bir potansiyele sahip olduğunu göstermiştir.

Anahtar Kelimeler

Soğuk katlama, dormansi, giberellik asit, potasyum nitrat, tohum

Proje Numarası

AR-1644

Kaynakça

• Abdel-Rahman RF, Algasoumi S, El-Desoky A, Soliman G, Pare G, Hegazy ME, 2015. Evaluation of the anti-inflammatory, analgesic and anti-ulcerogenic potentials of Achillea fragrantissima. South African Journal of Botany, 98: 122-127.
• Abdin MZ, Zhu Y, Tan B, Bay B, Liu C, 2007. Enhancing bioactive molecules in medicinal plants. Natural Products, 3: 45-57.
• Acikgoz MA, 2017. The determination of secondary metabolite production potential with callus culture in the species of Achillea gypsicola. Ordu University, Institute of Science, PhD Thesis (Printed).
• Almadiy AA, Nenaah GE, Al-Assiuty BA, Moussa EA, Mira NM, 2016. Chemical composition and antibacterial activity of essential oils and major fractions of four Achillea species and their nanoemulsions against foodborne bacteria. LWT-Food Science and Technology, 69: 529-537.
• Baser KHC, 2016. Essential oils of Achillea species of Turkey. Natural Volatiles & Essential Oils, 3: 1-14.
• Bhardwaj AK, Kapoor S, Naryal A, Bhardwaj P, Warghat AR, Kumar B, Chaurasia OP, 2016. Effect of various dormancy breaking treatments on seed germination, seedling growth and seed vigor of medicinal plants. Tropical Plant Research, 3: 508-516.
• Bourgaud F, Gravot A, Milesi S, Gontier E, 2001. Production of plant secondary metabolites, a historical perspective. Plant Science, 5: 839-851.
• Chuanren D, Bouchu V, Vangian L, Jing C, Lie L, Huan NZ, 2004. Effect of chemical and physical factors to improve the germination rate of Echinacea angustifolia seeds. Colloids and Surfaces B: Biointerfaces, 37: 101-105.
• Cirak C, Kevseroğlu K, Ayan AK, 2007. Breaking of seed dormancy in a Turkish endemic Hypericum species: Hypericum aviculariifolium depilatum var. depilatum by light and some pre-soaking treatments. Journal of Arid Environments, 68: 159-164.
• Delian E, Lagunovschi-Luchian V, 2015. Germination and vigour of primed Daucus carota L. seeds under saline stress conditions. Romanian Biotechnological Letters, 20: 10833- 10840.
• Demirci B, Başer KHC, Aytaç Z, Khan SI, Jacob MR, Tabanca N, 2018. Comparative study of three Achillea essential oils from eastern part of Turkey and their biological activities. Records of Natural Products, 12: 195-200.
• Elhindi KM, Dewir YH, Asrar AW, Abdel-Salam E, El-Din AS, Ali M, 2016. Improvement of seed germination in three medicinal plant species by plant growth regulators. Horticulture Science, 51: 887-891.
• Fariman ZK, Azizi M, Noori S, 2011. Seed germination and dormancy breaking techniques for Echinacea purpurea L. Journal of Biological and Environmental Sciences, 5: 7-10.
• Frizzo CD, Santos AC, Paraul N, Serafini LA, Dellacassa E, Lorenzo D, Moyna P, 2000. Essential oils of camphor tree (Cinnamomum camphora Nees and Eberm) cultivated in southern Brazil. Journal of Brazilian Archives of Biology and Technology, 43: 313-316.
• Gashi B, Abdullai K, Mata V, Konglika E, 2012. Effect of gibberellic acid and potassium nitrate on seed germination of the resurrection plants Ramonda serbica and Ramonda nathaliae. African Journal of Biotechnology, 11: 4537-4542.
• Ghasemi PA, 2017. Chemical composition of the essential oils from the leaves and flowers of two Achillea species from Iran. Journal of Essential Oil Bearing Plants, 20: 205-214.
• Gupta SM, Pandey P, Grover A, Ahmed Z, 2011. Breaking seed dormancy in Hippophae salicifolia, a high value medicinal plant. Physiology and Molecular Biology of Plants, 17: 403-406.
• Gupta V, 2003. Seed germination and dormancy breaking techniques for indigenous medicinal and aromatic plants. Journal of Medicinal and Aromatic Plants Science, 25: 402-407.
• Kadi K, Alaoui MM, Hamli S, Addad D, Dib D, Yahia A, 2015. Seed germination and dormancy breaking techniques for white henbane (Hyoscyamus albus L.). Advances in Environmental Biology, 9: 17-22.
• Kolumbina M, Blesing M, Mares DJ, 2006. α-Amylase and programmed cell death in aleurone of ripening wheat grains. Journal of Experimental Botany, 57: 877-885.
• McCrate AJ, Nielsen MT, Paulsen GM, Heyne EG, 1981. Gibberellic acid effects on germination and α amylase activity of winter wheats. Euphytica, 30: 875-880.
• Murashige T, Skoog F, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiology, 15: 473-497.
• Nadjafi F, Bannayan M, Tabrizi L, Rastgoo M, 2006. Seed germination and dormancy breaking techniques for Ferula gummosa and Teucrium polium. Journal of Arid Environments, 64: 542-547.
• Paunescu A, 2009. Biotechnology for Endangered Plant Conservation: A Critical Overview. Romanian Biotechnological Letters, 14: 4095-4103.
• Raisi A, Kalat SN, Darban AS, 2013. The study effects of stratification, temperature and potassium nitrate on seed dormancy breaking. World Applied Sciences Journal, 21, 379-383.
• Sampietro DA, Lizarraga EF, Ibatayev ZA, Omarova AB, Suleimen YM, Catalan CA, 2016. Chemical composition and antimicrobial activity of essential oils from Acantholippia deserticola, Artemisia proceriformis, Achillea micrantha and Libanotis buchtormensis against phytopathogenic bacteria and fungi. Natural Product Research, 30: 1950-1955.
• Steel RGD, Torrie JH, 1980. Principles and procedures of statistics, second edition. McGraw-Hill Publishing Company, New York, USA.
• Tabanca N, Demirci B, Aytaç Z, Başer KHC, 2016. Chemical composition of Achillea schischkinii Sosn. an endemic species from Turkey. National Volatiles & Essential Oils, 3: 24-28.
• Uludag A, Aksoy N, Yazlik A, Arslan ZF, Yazmis E, Uremis I, Brundu G, 2017. Alien flora of Turkey: checklist, taxonomic composition and ecological attributes. NeoBiota, 35: 61–85.
• Warakagoda PS, Subasinghe S, 2014. In vitro Seed Germination of Coscinium fenestratum (Gaertn.). Annual Research & Review in Biology, 4: 3549-3565.
• Zahed SY, Ramin AA, Baninasab B, 2015. Effect of gibberellic acid, stratification and salinity on seed germination of Echinacea purpurea cv. Magnus. Herba Polonica, 61: 13-22.
• Zare AR, Solouki M, Omidi M, Irvani N, Abasabadi AO, Nezad NM, 2011. Effect of various treatments on seed germination and dormancy breaking in Ferula assa foetida L. (Asafetida), a threatened medicinal herb. Trakia Journal of Sciences, 9: 57-61.
• Zuccarini P, 2009. Camphor: Risks and benefits of a widely used natural product. Journal of Applied Science of Environmental Management, 2: 69-74.