Floresans Enerji Transferi Üzerine Grafen Oksitin Kuençleştirici Etkisinin İncelenmesi

Yıl 2022, Cilt: 12 Sayı: 2, 870 - 881, 01.06.2022

Burcu Meryem Beşer

https://doi.org/10.21597/jist.1039599

Öz

Pirazolin türevi iki izomer molekül (4.2 ve 2.2) için sodyum dodesil sülfat misel ortamında seçilen dört farklı floresans probla (Safranin T, Akridin O, Pyronin Y, Floresin) gerçekleştirilen floresans enerji transferine bir floresans sönümleyici olduğu bilinen grafen oksitin kuençleştirici etkisi incelenmiştir. Yapılan çalışma durgun hal floresans spektroskopi tekniği ile gerçekleştirilmiştir. Enerji transferi ile ilgili parametreler Förster kinetiğine, kuençleşme ile ilgili parametreler ise Stern-Volmer kinetiğine göre belirlenmiştir. Çalışmanın sonucunda donör-akseptör çiftleri arasındaki floresans enerji transferinin GO tarafından zayıflatıldığı fakat tamamen sonlanmadığı ve düşük oranda devam ettiği anlaşılmıştır.

Anahtar Kelimeler

Floresans enerji transferi, grafen oksit, kuençleşme, pirazolin, Stern-Volmer

Investigation of the Quenching Effect of Graphene Oxide on Fluorescence Energy Transfer

Öz

The quenching effect of GO on the fluorescence energy transfer between two pyrazoline-derived isomer molecules (4,2 and 2,2) (donor) and four different acceptor molecules (Safranin T, Acridine O, Pyronin Y, Fluorescein) were investigated in sodium dodecyl sulfate micellar media. The study was carried out using the steady state fluorescence spectroscopy technique. Parameters related to energy transfer were determined according to Förster kinetics, and parameters related to quenching were determined according to Stern-Volmer kinetics. As a result of the study, it was understood that the fluorescence energy transfer between donor-acceptor pairs was weakened by GO, but not completely terminated and continued at a low rate.

Anahtar Kelimeler

Fluorescence energy transfer, graphene oxide, quenching, pyrazoline, Stern-Volmer

Kaynakça

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