Seasonal Changes of FSH, LH, Total and Free Testosterone Hormones in Saanen Bucks

Öz

Araştırmada, Saanen tekelerine ait folikül uyarıcı hormon (FSH), lutein hormon (LH), toplam testosteron (TTH) ve Serbest Testosteron Hormonunun (FTH) mevsimsel değişiklikleri incelenmiştir. Şubat 2019 ile Aralık 2019 arasındaki aylarda hormon düzeylerinin belirlenmesi için tekelerden (n=6) haftada iki kez olmak üzere tüm deneme süresince kan örnekleri alındı. FSH düzeyi, ağustos ayından sonra göreli olarak düşmeye başlamış ve en düşük değer ocak ayında 173.01 mlU/ml olarak belirlenmiştir. FSH düzeyi ortalamalarında aylar arasındaki fark anlamlı bulunmuştur (p <0.05). En yüksek LH düzeyi Temmuz (13.12 mIU/mL) ve en düşük düzey ise (8.02 mIU/mL) mayıs ayında elde edilmiştir. Ancak çiftleşme mevsiminde, LH düzeyindeki artışın FSH düzeyiyle benzer olduğu saptanmıştır. Aylara göre LH düzeylerindeki fark istatistiksel olarak önemli bulunmuştur (p<0.05). Toplam testosteron düzeyi, FSH ve LH düzeyi ile benzerlik göstermiştir. Mart ve ocak aylarında en yüksek ve en düşük toplam testosteron hormonu (TTH) düzeyi sırasıyla; 3.03 ng-/mL ve 0.62 ng-/-mL dir. En yüksek Serbest testosteron hormonu (FTH) düzeyi ağustos ayında 119.69 pg/ml, en düşük ise aralık ayında 29.09 pg/ml olarak belirlenmiştir. Aylara göre FTH arasındaki fark anlamlıdır (p<0.05). Saanen tekelerinde üreme hormonlarının mevsimsel değişimi üzerine gün uzunluğunun etkisi istatistiki olarak önemlidir (p<0.05). Sonuç olarak, Saanen tekelerinde üreme hormonu düzeylerinin mevsime bağlı olduğu ve salım düzeylerinin gün uzunluğu gibi iklimsel faktörlerden önemli ölçüde etkilendiği belirlenmiştir. Özetlemek gerekirse, tekelerde erkek üreme özellikleri ve diğer önemli hormonların etkilerini daha ayrıntılı incelemek ve sonuçların daha sağlıklı yorumlanabilmesi için örnek alma sıklığı, örnek alma zamanının yanı sıra örnek büyüklüğü ve diğer çevresel faktörlerin de içinde yer aldığı daha farklı çalışmalara da ihtiyaç vardır.

Anahtar Kelimeler

• Saanen teke
• Üreme hormonu
• Mevsimsel değişim
• Serbest testesteron hormonu
• Yıllık döngü

Proje Numarası

2016-ZRF-070

Seasonal Changes of FSH, LH, Total and Free Testosterone Hormones in Saanen Bucks

Öz

In the study, seasonal changes in follicle-stimulating hormone (FSH), lutein hormone (LH), total testosterone (TTH), and Free Testosterone Hormone (FTH) of Saanen goats were investigated. In order to determine the hormone levels between February 2019 and December 2019, blood samples were taken from bucks (n=6) twice a week during the entire trial. FSH level started to decrease relatively after August and the lowest value was determined as 173.01 mlU/ml in January. The difference between the months in the mean FSH level was found to be significant (p <0.05). The highest LH level was obtained in July (13.12 mIU/mL) and the lowest level (8.02 mIU/mL) was obtained in May. However, during the mating season, the increase in LH level was found to be similar to the FSH level. The difference in LH levels by months was found to be statistically significant (p<0.05). Total testosterone level was similar to FSH and LH levels. The highest and lowest total testosterone hormone (TTH) levels in March and January, respectively; 3.03 ng-/mL and 0.62 ng-/-mL. The highest free testosterone hormone (FTH) level was 119.69 pg/ml in August and the lowest was 29.09 pg/ml in December. The difference between FTH by months was significant (p<0.05). The effect of day length on seasonal variation of reproductive hormones in Saanen bucks is statistically significant (p<0.05). As a result, it was determined that reproductive hormone levels in Saanen bucks depend on the season and the release levels are significantly affected by climatic factors such as day length. To summarize, there is a need for more different studies including sampling frequency, sampling time as well as sample size, and other environmental factors in order to examine the effects of male reproductive characteristics and other important hormones in bucks in more detail to interpret the results more accurately.

Anahtar Kelimeler

• Saanen buck
• Reproductive hormone
• Seasonal change
• Free testosterone hormone
• Annual cycle

Destekleyen Kurum

Ege University Scientific Research Projects Directorate

Proje Numarası

2016-ZRF-070

Teşekkür

This study (2016-ZRF-070) was supported by Ege University Scientific Research Projects Directorate under Grant 2016-2019.

Kaynakça

1. Abdelrahman, S.S., Abdalla, M.S.A., Darderi, T.G., Ali, E.A.E. (2019). Development of hormonal profiles of Nubian bucks at puberty in Sudan. Journal of Veterinary Medicine and Animal Health, 11(6):106-114.
2. Abecia, J.A., Forcada, F., Gonzalez-Bulnes, A. (2012). Hormonal control of reproduction in small ruminants. Animal Reproduction Science, 130: 173– 179.
3. Al-Ghalban, A.M., Tabbaa, M.J., Kridli, R.T. (2004). Factors affecting semen characteristics and scrotal circumference Damascus buck. Small Ruminant Reserch, 53:141–149.
4. Araki, K., Arai, K.Y., Watanabe, G., Taya, K. (2000). Involvement of inhibin in the regulation of follicle-stimulating hormone secretion in the young adult male Shiba Goat. Journal of Andrology, 21(4): 558–565.
5. Barkawi, A.H., Elsayed, E.H., Ashour, G., Shehata, E. (2006). Seasonal changes in semen characteristics, hormonal profiles and testicular activity in Zaraibi goats. Small Ruminant Reserch, 66: 209-213.
6. Cattanach, B.M., Iddon, C.A., Charlton, H.M., Chiappa, S.A., Fink, G. (1977). Gonadotrophin-releasing hormone deficiency in a mutant mouse with hypogonadism. Nature, 269: 338–340.
7. Chemineau, P., Pelletier, J., Guerin, Y., Colas, G., Ravault, J.P., Toure, G., Almeida, G., Thimonier, J., Ortovant, N. (1988). Photoperiodic and melatonin treatments for the control of seasonal reproduction in sheep and goats. Reproduction Nutrition Development, 28(2B): 409-422.
8. Cheng, K.W., Simaraks, S., Palmer, W.M. (1981). Characterization of a radioimmunoassay for ovine FSH utilizing an anti-bovine FSH serum. Journal of Reproduction & Infertility, 61:115-121.

9. Chentouf, M., Bister, J.L., Boulanouar, B. (2011). Reproduction characteristics of North Moroccan indigenous goats. Small Ruminant Reserch, 98:185–188.
10. Coloma, M.A., Toledano-Díaz, A., Castaño, C., Velázquez, R., Gómez- Brunet, A., López-Sebastián, A., Santiago-Moreno, J. (2011). Seasonal variation in reproductive physiological status in the Iberian ibex (Capra pyrenaica) and its relationship with sperm freezability. Theriogenology,76: 1695–1705.
11. Culler, M.D., Negro-Vilar, A. (1988). Passive immunoneutralization of endogenous inhibin: sex-related differences in the role of inhibin during development. Molecular and Cellular Endocrinology, 58:263–273.
12. Delgadillo, J.A., Canedo, G.A., Chemineau, P., Guillaume, D., Malpaux, B. (1999). Evidence for an annual reproductive rhythm independent of food availability in male Creole goats in subtropical northern Mexico. Theriogenology, 52(4): 727-737.
13. Delgadillo, J.A., Carrillo, E., Morán, J., Duarte, G., Chemineau, P., Malpaux B., Mora, J. (2001). Induction of sexual activity of male creole goats in subtropical northern Mexico using long days and melatonin. Journal of Animal Science, 79 (2): 2245-2252.
14. Delgadillo, J.A., Fitz-Rodriguez, B., Duarte, G., Malpaux, B. (2004). Management of photoperiod to control carpine reproduction in the subtropics. Reproduction, Fertility and Development, 16: 471- 478.
15. Delgadillo, J.A., Flores, J.A., Véliz, F.G., Hernández, H.F., Duarte, G., Vielma, J., Poindron, P., Chemineau, P., Malpaux, B. (2002). Induction of sexual activity in lactating anovulatory female goats using male goats treated only with artificially long days. Journal of Animal Science, 80 (11): 2780-2786.
16. Dellal, G., Cedden, F. (2012). Reproduction in farm animals. In: Hayvan Yetiştirme (Ed: Ertuğrul, M.). Anadolu Üniversitesi, Açık Öğretim Fakülte (Yayın No: 1252) Eskişehir.
17. Dias, J.C.O., Veloso, C.M., Santos, M.C.da R., de Olivera, C.T.S.A.M., Silvera, C.O., Iglesias, E., Maitan, P.P., Sanglard, L.M.P. (2012). Seasonal variation in the reproductive activity of male goats raised under tropical climate conditions. Revista Brasileira de Zootecnia, 46(3):192-201.
18. Eldon, J. (1993). Effect of exogenous melatonin and exposure to a ram on the time of onset and duration of the breeding season in Icelandic sheep. Journal of Reproduction & Fertility, 99:1-6. Fatet, A., Pellicer-Rubio, M.T., Leboeuf, B. (2011). Reproductive cycle of goats. Animal Reproduction Science, 124 (3-4): 211–219.
19. Findlay, J.K., Clarke, I.J. (1987). Regulation of FSH in domestic animals. Journal of Reproduction & Fertility, 34 (Suppl.) 27–37.
20. Gomez-Brunet, A., Santiago-Moreno, J., Toledano-Dia, A., López-Sebastián, A. (2009). Sperm variables as predictors of fertility in Black Castellana roosters; use in the selection of sperm donors for genome resource banking purposes. Spanish Journal of Agricultural Research, 7(3):555-562.
21. Goodman, R.L., Inskeep, E.K. (2006). Neuroendocrine Control of the Ovarian Cycle of the Sheep. P.2389-2428. In: Physiology of Reproduction. Elsevier, London.
22. Gürbüz, F., Başpınar, E., Çamdeviren, H., Keskin, S. (2003). Tekrarlanan Ölçümlü Deneme Düzenlerinin Analizi. Yüzüncü Yıl Üniversitesi Matbaası, Van.
23. Howland, B.E., Sanford, L.M., Palmer, W.M. (1985). Changes in serum levels of LH, FSH, prolactin, testosterone, and cortisol associated with season and mating in male pygmy goats. Journal of Andrology, 6: 89-96.
24. Kafi, M., Safdarian, M., Hashemi, M. (2004). Seasonal variation in semen characteristics, scrotal circumference and libido of Persian Karakul rams. Small Ruminnant Reserch, 53:133-139.
25. Karadağ, O.,Soysal, M.İ. (2018). The determination of some, reproduction, growth and morphological traits in Honamlı goats breeds. Journal of Tekirdag Agricultural Faculty, 15(1):135-142
26. Kandemir, Ç., Taskin, T., Koşum, N. (2018). A Study on determination of some yield traits of saanen goats in ıntensive conditions. Journal Animal Production, 59 (1): 41-49.
27. Kaneko, H., Nakanishi, Y., Akagi, S., Arai, K., Taya, K., Watanabe, G., Sasamoto, S., Hasegawa, Y. (1995). Immunoneutralization of inhibin and estradiol during the follicular phase of the estrous cycle in cows. Biology of Reproduction, 53: 963–971.
28. Kishi, H., Okada, T., Kawazu, S., Otsuka, M., Taya, K., Watanabe, G., Sasamoto, S. (1997). Effects of passive immunization against oestradiol-17beta and inhibin on the secretion of gonadotrophin in the cyclic golden hamster (Mesocricetus auratus). Reproduction, Fertility and Development, 9: 447–453.
29. Koluman Darcan, N., Daşkıran, İ., Şener, B. (2013). The heat strees effect on T4 (Thyroxin), T3 (Triiodothyronine), Cortisol hormones of goats in rearing extensive systems. Journal of Tekirdag Agricultural Faculty, 10 (3), 29-36.
30. Kumar, T.R., Wang, Y., Lu, N., Matzuk, M.M. (1997). Follicle stimulating hormone is required for ovarian follicle maturation but not male fertility. National Genetic, 15:201–204.
31. Loubser, P.G., Van Niekerk, C.H., Botha, L.J.J. (1983). Seasonal changes in sexual activity and semen quality in the Angora ram. l. Libido and male hormone concentrations. South African Journal of Animal Science, 13(2): 131-133.
32. Malpaux, B., Viguie, C., Ravault, J.P., Thiery, J.C., Chemineau, P. (1994). Photoperiodic and Neuroendocrine Control of Seasonal Reproductive Functions in The Ovine and Caprine Species. European Fine Fiber Network, Occasional Publication No: 2.
33. Mann, G.E., Cambell, B.K., McNeilly, A.S., Baird, D.T. (1990). Effects of passively immunizing ewes against inhibin and estradiol during the follicular phase of the estrous cycle. Journal of Endocrinology, 125:417–424.
34. Marai, I.F.M., El-Darawany, A.A., Fadiel, A., Abdel-Hafez, M.A.M. (2007). Physiological traits as affected by heat stress in sheep-a review. Small Ruminant Research, 71: 1-12.
35. Martin, T.L., Williams, G.L., Lunstra, D.D., Ireland, J.J. (1991). Immunoneutralization of inhibin modifies hormone secretion and sperm production in bulls. Biology of Reproduction, 45:73–77.
36. Mayer, C.J. (2016). The Roles of Testicular Testosterone and Inhibin in The Negative-Feedback Regulation of GnRH, LH and FSH. http://thedarkpark.wordpress.com/2011/10/18/ the-roles-of-testicular-testosterone-and-inhibin-in-the-negative-feedback- regulation-of-GnRH-LH-and-FSH/, retrieved 11-09-2016.
37. McKeown, R.M., O’Callaghan, D., Roche, J.F., Boland, M.P. (1997). Effect of immunization of rams against bovine inhibin alpha 1–26 on semen characteristics, scrotal size, FSH, LH and testosterone concentrations. Journal of Reproduction & Fertility,109: 237–245.
38. Medan, M.S., Watanabe, G., Sasaki, K., Nagura, Y., Sakaime, H., Fujita, M., Sharawy, S., Taya, K. (2003). Ovarian and hormonal response of female goats to active immunization against inhibin. Journal of Endocrinology, 177: 287–294.
39. Mori, Y., Kano, Y. (1984). Changes in plasma concentrations of LH, progesterone and estradiol in relation to the occurrence of luteolysis, estrus and time of ovulation in Shiba goats (Capra hircus). Journal of Reproduction & Fertility, 72: 223–230.
40. Muduuli, D.S., Sanford, L.M., Palmer, W.M., Howland, B.E. (1979). Secretory Patterns and circadian and seasonal changes in LH, FSH, prolactin and testosterone in the male Pygmy goat. Journal of Animal Science, 49(2): 11-19.
41. Nakao, N., Ono, H., Yoshimura, T. (2008). Нyroid hormones and seasonal reproductive neuroendocrine interactions. Reproduction, 136: 1-8.
42. Nikbin, S., Panandam, J.M., Yaakub, H., Murugaiyah, M. (2018). Association of novel SNPs in gonadotropin genes with sperm quality traits of Boer goats and Boer crosses. Journal of Applied Animal Research, 46(1):459–466.
43. NRC. (2007). Nutrient Requirements of Small Ruminants. National Research Council of the National Academies.
44. Pehlivan, E., Polat, H., Dellal, G. (2017). Annual change of reproductive hormones in angora goat bucks. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, 57 (1): 18-24.
45. Perez, B., Mateos, E. (1995). Seasonal variations in plasma testosterone levels in Verata and Malaguena bucks. Small Rumminant Reserch, 15: 155-162.
46. Polat, H., Dellal, G., Barıtçı, İ., Pehlivan, E. (2011). Annual change of the testosterone hormone in male white goats. Agricultural Sciences in China, 10(2): 312-316.
47. Quin, D.N., She, B.R., Shei, Y., Wang, J.H. (2000). Effect of flavonoids from Semen Cuscutae on the reproductive system in male rats. Asian Journal of Andrology, 2:99-102.
48. Ritar, A.J. (1991). Seasonal changes in LH, androgens and testes in the male Angora goat. Theriogenology, 36(6): 959–972.
49. Rosa, H.J.D., Bryant, M.J. (2003). Seasonality of reproduction in sheep. Small Ruminnant Reserch, 48:155-171.
50. Sharpe, R.M. (2003). Proliferation and functional maturation of sertoli cells, and their relevance disorders of testis function in adulthood. Reproduction, 125:769-784.
51. Şenok, A., Pehlivan, E., Cedden, F. (2020). Effect of buck existence on some reproductive hormone levels during premating in Akkeçi goats. Turkish Journal of Veterinary & Animal Sciences, 45(5): Article 16.
52. Todini, L., Malfatti, A., Terzano, G.M., Borghese, A., Pizzillo, M., Debenedetti, A. (2007). Seasonality of plasma testosterone in males of four Mediterranean goat breeds and in three different climatic conditions. Theriogenology, 67: 627–631.
53. Vasantha, I. (2016). Physiology of seasonal breeding: a review. Journal of Veterinary Science and Technology, 7:3, 1-4.
54. Walkden-Brown, S.W., Restall, B.J., Norton, B.W., Scaramuzzi, R.J., Martin, G.B. (1994). Effect of nutrition on seasonal patterns of LH, FSH and testosterone concentration, testicular mass, sebaceous gland volume and odor in Australian cashmere goats. Journal of Reproduction & Fertility, 102: 351-360.
55. Yarney, T.A., Sanford, L.M. (1983). The reproductive endocrine response of adult rams to sexual encounters with estrual ewes season dependent. Hormones and Behavior, 17: 169-182.
56. Yılmaz, B. (1999). Hormones and Reproductive Physiology. A.Ü. Veteriner Fak. Fizyoloji Anabilim Dalı. 1. Basım, Ankara.
57. Zamiri, M.J., Khodaei, H.R. (2005). Seasonal thyroidal activity and reproductive characteristics of Iranian fat-tailed rams. Animal Reproduction Science, 88: 245-255.
58. Zarazaga, L.A., Guzman, J.L., Dominguez, C., Perez, M.C., Prieto, R. (2009). Effects of season and feeding level on reproductive activity and semen quality in Payoya buck goats. Theriogenology, 71:1316–1325.
59. Zhang, Z.B., Yang, Q.T. (2006). The testosterone mimetic properties of icariin. Asian Journal of Andrology, 8(5): 601-605.