Aronia Melanocarpa (Michx.) Meyvesindeki Fenolik, Flavonoid, Toplam Antioksidan Kapasitesi, Vitamin ve Glutatyon İçeriğinin Araştırılması

Yıl 2025, Cilt: 15 Sayı: 3, 1240 - 1251, 15.09.2025

Refik Servi , Fikret Karataş , Dursun Özer , Sinan Saydam

https://doi.org/10.31466/kfbd.1659652

Öz

Aronya meyvesindeki yağda ve suda gözünen vitaminler, glutatyon miktarları yüksek performanslı sıvı kromatografisi (HPLC) ile tayin edilmiştir. Toplam fenolik ve flavonoid bileşik miktarları ve toplam antioksidan kapasitesi spektrofotometre ile belirlenmiştir. Meyvedeki vitamin A, E, β-karoten ve likopen miktarları sırasıyla 12.44±0.40, 19.51±0.51, 51.64±1.41 ve 5.85±0.17 µg/g olarak bulundu. Aronya meyvesindeki suda çözünen vitaminlerden askorbik asit, tiamin, riboflavin, nikotinik asit, pantotenik asit, pridoksin, folik asit ve siyanokobalamin miktarları sırasıyla 412.92±7.29, 4.27±0.14, 16.43±0.48, 34.13±1.01, 36.37±0.59, 3.43±0.12, 14.0±0.55, 5.33±0.14 µg/g, iken redükte ve okside glutatyon (GSH, GSSG) miktarları ise 706.32±11.25, 133.67±4.79 µg/g olarak belirlendi. Meyvelerdeki toplam fenolik madde miktarı 81.28±1.86 µg Gallik Asit/g, flavonoid madde miktarı ise 65.55±1.45 µg Kuersettin/g olarak belirlenmiştir. Aronya meyvesindeki DPPH yöntemine göre belirlenen IC50 değeri 23.17±0.78 µg/mL, TEAK yöntemine göre hesaplanan antioksidan kapasite değeri ise 272.85±6.31 µmol Troloks/g olarak bulunmuştur. Olgun aronya meyvesindeki vitaminler, glutatyon, toplam fenolik ve flavonoid madde miktarları ile toplam antioksidan kapasite bakımından uygun dozda tüketildiğinde sağlık için yararlı olduğu söylenebilir.

Anahtar Kelimeler

Aronya melanocarpa (Michx.) , Vitaminler , Glutatyon , Fenolik madde , Toplam antioksidan kapasite

Investigation of Phenolic, Flavonoid, Total Antioxidant Capacity, Vitamins, and Glutathione Contents of Aronia Melanocarpa (Michx.) Fruits

Öz

The nutritional profile and antioxidant capacity of ripe aronia (Aronia melanocarpa) fruit were comprehensively analyzed using High-Performance Liquid Chromatography (HPLC) and Ultraviolet-visible (UV-vis) spectrophotometry. The content of fat-soluble vitamins was quantified as follows: vitamin A – 12.44 ± 0.69 µg/g, vitamin E – 19.51 ± 0.88 µg/g, β-carotene – 51.64 ± 2.44 µg/g, and lycopene – 5.85 ± 0.30 µg/g. Water-soluble vitamins were also abundant, with ascorbic acid (vitamin C) being the most prevalent at 412.92 ± 12.61 µg/g, followed by riboflavin (16.43 ± 0.83 µg/g), pantothenic acid (36.37 ± 1.02 µg/g), and others including thiamine, nicotinic acid, pyridoxine, folic acid, and cyanocobalamin. Glutathione levels, essential for redox balance, were measured as 706.32 ± 19.46 µg/g for reduced glutathione (GSH) and 133.67 ± 8.28 µg/g for oxidized glutathione (GSSG). The fruit also exhibited high polyphenol and flavonoid contents, with total phenolics at 81.28 ± 3.21 µg gallic acid equivalents per gram and flavonoids at 65.55 ± 2.50 µg quercetin equivalents per gram. Antioxidant capacity was evaluated using two methods: the DPPH assay yielded an IC₅₀ value of 23.17 ± 1.35 µg/mL, indicating strong radical scavenging activity, while the TEAC method demonstrated a total antioxidant capacity of 272.85 ± 10.91 µmol Trolox equivalents per gram. Ripe aronia fruit is rich in essential vitamins, glutathione, phenolic and flavonoid compounds, and possesses strong antioxidant capacity. These findings support its potential health benefits when consumed in appropriate amounts.

Anahtar Kelimeler

Aronia melanocarpa (Michx.) , Vitamins , Glutathione , Phenolic substance , Flavonoid substance , Total antioxidant capacity

Etik Beyan

Gerekli Değil

Kaynakça

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