Effect of Pine Cone Vinegar on The Survival of Listeria monocytogenes and Salmonella typhimurium and Some Physico-Chemical Properties in Raw Beef

Öz

Pine cones from pine (Pinus) trees contain various bioactive compounds with antibacterial and antioxidant activity. However, the use of pine cone products as functional foods or food additives is limited and there is a paucity of research in the literature. The objective of this study was the evaluation of pine cone vinegar as a marinade against Listeria monocytogenes and Salmonella typhimurium in raw beef. For this purpose, raw beef was marinated with pine cone vinegar at three different levels (25%, 50% and 75%) and three different marination times (2, 6 and 24 hours) and L. monocytogenes, S. typhimurium counts, pH and colour values (L*, a*, b*) were determined. Concentration*time interaction was significant for pH and the lowest pH value was found at 75% x 24 hours interaction. Although the marinating process had a significant effect on the colour values of the meat, the concentration x time interaction was not significant for the a* value (p>0.05). The number of S. typhimurium in pine cone vinegar decreased by 3.7 log10 in 2 hours and by 5.26 log10 in 6 hours (p<0.05). The number of L. monocytogenes decreased by 4.17 log10 in 2 hours and 5.29 log10 in 6 hours (p<0.05). The number of S. typhimurium and L. monocytogenes decreased significantly in marinated beef samples, with the greatest decrease being observed at 24 hours for all concentrations. After 24 hours of marination, Salmonella counts decreased by 1.69, 1.68 and 1.73 log10, and L. monocytogenes counts decreased by 3.6, 3.43 and 3.13 log10, respectively. The findings of this study indicate that pine cone vinegar can serve as an effective decontaminant for meat and meat products, making it a valuable component in marinades.

Anahtar Kelimeler

• Beef
• Listeria monocytogenes
• Marinating
• Pine cone
• Salmonella typhimurium

Çam Kozalağı Sirkesinin Çiğ Sığır Etinde Listeria monocytogenes ve Salmonella typhimurium'un Hayatta Kalması ve Bazı Fiziko-Kimyasal Özellikler Üzerine Etkisi

Öz

Çam (Pinus) ağacı türlerinden elde edilen çam kozalakları antibakteriyel ve antioksidan aktiviteye sahip çeşitli biyoaktif maddelere sahiptir. Ancak çam kozalağından elde edilen ürünlerin fonksiyonel gıda veya gıda katkı maddesi olarak kullanımı sınırlıdır ve literatürde bu konuda çok fazla araştırma bulunmamaktadır. Bu çalışmanın amacı da çam kozalağı sirkesinin çiğ sığır etlerinde Listeria monocytogenes ve Salmonella typhimurium karşı bir marinat olarak değerlendirmektir. Bu amaçla çam kozalağı sirkesi ile üç farklı konsantrasyon (%25, %50 ve %75) ve 3 farklı marinasyon süresininde (2, 6 ve 24 saat) çiğ sığır eti marine edilip, L. monocytogenes, S. typhimurium sayıları, pH ve renk değerleri (L*, a*, b*) belirlendi. pH için konsantrasyon*zaman etkileşimi önemli olduğu, en düşük pH değeri %75 x 24 saat etkileşiminde sapandı. Marinasyon işleminin etin renk değerleri üzerinde önemli etkileri olmasına rağmen, a* değeri için konsantrasyon x zaman etkileşimi önemli olmadığı belirlendi (p>0,05). Çam kozalağı sirkesinde S. typhimurium sayısının 2 saatte 3,7 log 10, 6 saatte ise 5,26 log 10 azaldığı tespit edildi (p<0,05). L. monocytogenes sayısının ise 2 saatte 4,17 log 10, 6 saatte ise 5,29 log 10 azaldığı tespit edildi (p<0,05). Marine edilmiş sığır eti örneklerinde S. typhimurium ve L. monocytogenes sayısının önemli düzeyde azaldığı en çok azalma tüm konsantrasyonlarda 24 saatte tespit edildi. 24 saat marinasyon sonrası Salmonella sayısı sırasıyla 1,69, 1,68 ve 1,73 log 10 azaldığı, L. monocytogenes sayısı ise sırasıyla 3,6, 3,43 ve 3,13 log 10 azaldığı saptandı. Bu çalışmanın sonuçları, çam kozalağı sirkesinin et ve et ürünlerinin dekontaminasyonu için kullanılabileceği ve marinatların önemli bir bileşeni olabileceğini ortaya koymuştur.

Anahtar Kelimeler

• Çam kozalağı
• Listeria monocytogenes
• Marinasyon
• Sığır eti
• Salmonella typhimurium

Kaynakça

1. Aminzare, M., Hashemi, M., Hassanzadazar, H., & Hejazi, J. (2016). The use of herbal extracts and essential oils as a potential antimicrobial in meat and meat products; a review. Journal of Human Environment and Health Promotion, 1(2), 63-74.
2. Bakir, S., Devecioglu, D., Kayacan, S., Toydemir, G., Karbancioglu-Guler, F., & Capanoglu, E. (2017). Investigating the antioxidant and antimicrobial activities of different vinegars. European Food Research and Technology, 243, 2083-2094.
3. Barbut, S., Sosnicki, A. A., Lonergan, S. M., Knapp, T., Ciobanu, D. C., Gatcliffe, L. J., ... & Wilson, E. W. (2008). Progress in reducing the pale, soft and exudative (PSE) problem in pork and poultry meat. Meat science, 79(1), 46-63. https://doi.org/10.1016/j.meatsci.2007.07.031
4. Budak, N. H., Aykin, E., Seydim, A. C., Greene, A. K., & Guzel‐Seydim, Z. B. (2014). Functional properties of vinegar. Journal of food science, 79(5), 757-764. https://doi.org/10.1111/1750-3841.12434
5. Choi, Y. M., Bae, Y. Y., Kim, K. H., Kim, B. C., & Rhee, M. S. (2009). Effects of supercritical carbon dioxide treatment against generic Escherichia coli, Listeria monocytogenes, Salmonella typhimurium, and E. coli O157: H7 in marinades and marinated pork. Meat Science, 82(4), 419-424. https://doi.org/10.1016/j.meatsci.2009.02.016
6. Chung, D., Cho, T. J., & Rhee, M. S. (2018). Citrus fruit extracts with carvacrol and thymol eliminated 7-log acid-adapted Escherichia coli O157: H7, Salmonella typhimurium, and Listeria monocytogenes: A potential of effective natural antibacterial agents. Food Research International, 107, 578-588. https://doi.org/10.1016/j.foodres.2018.03.011
7. Fencioglu, H., Oz, E., Turhan, S., Proestos, C., & Oz, F. (2022). The effects of the marination process with different vinegar varieties on various quality criteria and heterocyclic aromatic amine formation in beef steak. Foods, 11(20), 3251. https://doi.org/10.3390/foods11203251
8. İlhak, O. İ., İncili, G. K., & Durmuşoğlu, H. (2018). Effect of some chemical decontaminants on the survival of Listeria monocytogenes and Salmonella Typhimurium with different attachment times on chicken drumstick and breast meat. Journal of food science and technology, 55, 3093-3097. https://doi.org/10.1007/s13197-018-3234-7

9. İncili, G. K., Akgöl, M., Aydemir, M. E., Alan, S., Mutlu, M., İlhak, O. İ., & Öksüztepe, G. (2020). Fate of Listeria monocytogenes and Salmonella Typhimurium in homemade marinade and on marinated chicken drumsticks, wings and breast meat. LWT, 134, 110231. https://doi.org/10.1016/j.lwt.2020.110231
10. İncili, G. K., Aydemi̇r, M. E., Akgöl, M., Kaya, B., Kanmaz, H., Öksüztepe, G., & Hayaloğlu, A. A. (2021). Effect of Rheum ribes L. juice on the survival of Listeria monocytogenes, Escherichia coli O157: H7 and Salmonella Typhimurium and chemical quality on vacuum packaged raw beef. LWT, 150, 112016. https://doi.org/10.1016/j.lwt.2021.112016
11. Kaur, R., Kaur, L., Gupta, T. B., & Bronlund, J. (2023). Mānuka Oil vs. Rosemary Oil: Antimicrobial Efficacies in Wagyu and Commercial Beef against Selected Pathogenic Microbes. Foods, 12(6), 1333. https://doi.org/10.3390/foods12061333
12. Karam, L., Chehab, R., Osaili, T. M., & Savvaidis, I. N. (2020). Antimicrobial effect of thymol and carvacrol added to a vinegar-based marinade for controlling spoilage of marinated beef (Shawarma) stored in air or vacuum packaging. International Journal of Food Microbiology, 332, 108769. https://doi.org/10.1016/j.ijfoodmicro.2020.108769
13. Karatepe, P., Akgöl, M., İncili, C. A., Tekin, A., İncili, G. K., & Hayaloğlu, A. A. (2023). Effect of hawthorn vinegar-based marinade on the quality parameters of beef tenderloins. Food Bioscience, 56, 103098. https://doi.org/10.1016/j.fbio.2023.103098
14. Kelebek, H., Kadiroğlu, P., Demircan, N. B., & Selli, S. (2017). Screening of bioactive components in grape and apple vinegars: Antioxidant and antimicrobial potential. Journal of the Institute of Brewing, 123(3), 407-416. https://doi.org/10.1002/jib.432
15. Kim, J., Park, J. G., Lee, C. M., Kim, S. G., Kim, J., Park, J. G., ... & Kim, S. G. (2019). Effect of the pine cone extract phytochemical and physiological activity on HaCaT cells. Asian journal of beauty and cosmetology, 17(3), 353-363. http://dx.doi.org/10.20402/ajbc.2019.0303
16. Lopes, S. M., da Silva, D. C., & Tondo, E. C. (2022). Bactericidal effect of marinades on meats against different pathogens: A review. Critical Reviews in Food Science and Nutrition, 62(27), 7650-7658.
17. Luzón-Quintana, L. M., Castro, R., & Durán-Guerrero, E. (2021). Biotechnological processes in fruit vinegar production. Foods, 10(5), 945. https://doi.org/10.1080/10408398.2021.1916734
18. Lytou, A. E., Tzortzinis, K., Skandamis, P. N., Nychas, G. J. E., & Panagou, E. Z. (2019). Investigating the influence of organic acid marinades, storage temperature and time on the survival/inactivation interface of Salmonella on chicken breast fillets. International journal of food microbiology, 299, 47-57. https://doi.org/10.1016/j.ijfoodmicro.2019.03.019
19. Mazaheri Kalahrodi, M., Baghaei, H., Emadzadeh, B., & Bolandi, M. (2021). Degradation of myofibrillar and sarcoplasmic proteins as a function of marinating time and marinade type and their impact on textural quality and sensory attributes of m. semitendinosus beefsteak. Journal of Food Processing and Preservation, 45(9), e15691. https://doi.org/10.1111/jfpp.15691
20. Ozturk, I., Caliskan, O., Tornuk, F., Ozcan, N., Yalcin, H., Baslar, M., & Sagdic, O. (2015). Antioxidant, antimicrobial, mineral, volatile, physicochemical and microbiological characteristics of traditional home-made Turkish vinegars. LWT-Food Science and Technology, 63(1), 144-151. https://doi.org/10.1016/j.lwt.2015.03.003
21. Özen, M., Özdemir, N., Filiz, B. E., Budak, N. H., & Taş, T. K. (2020). Sour cherry (Prunus cerasus L.) vinegars produced from fresh fruit or juice concentrate: Bioactive compounds, volatile aroma compounds and antioxidant capacities. Food chemistry, 309, 125664. https://doi.org/10.1016/j.foodchem.2019.125664
22. Park, N. Y., Hong, S. H., & Yoon, K. S. (2014). Effects of commercial marinade seasoning and a natural blend of cultured sugar and vinegar on Campylobacter jejuni and Salmonella Typhimurium and the texture of chicken breasts. Poultry Science, 93(3), 719-727. https://doi.org/10.3382/ps.2013-03595
23. Pashazadeh, H., Özdemir, N., Zannou, O., & Koca, I. (2021). Antioxidant capacity, phytochemical compounds, and volatile compounds related to aromatic property of vinegar produced from black rosehip (Rosa pimpinellifolia L.) juice. Food Bioscience, 44, 101318. https://doi.org/10.1016/j.fbio.2021.101318
24. Purslow, P. P., Warner, R. D., Clarke, F. M., & Hughes, J. M. (2020). Variations in meat colour due to factors other than myoglobin chemistry; a synthesis of recent findings (invited review). Meat Science, 159, 107941. https://doi.org/10.1016/j.meatsci.2019.107941
25. Sengun, I. Y., Kilic, G., & Ozturk, B. (2020). Screening physicochemical, microbiological and bioactive properties of fruit vinegars produced from various raw materials. Food science and biotechnology, 29, 401-408. https://doi.org/10.1007/s10068-019-00678-6
26. Sengun, I. Y., Kilic, G., & Ozturk, B. (2020). The effects of koruk products used as marination liquids against foodborne pathogens (Escherichia coli O157: H7, Listeria monocytogenes and Salmonella Typhimurium) inoculated on poultry meat. LWT, 133, 110148. https://doi.org/10.1016/j.lwt.2020.110148
27. Sengun, I. Y., Turp, G. Y., Cicek, S. N., Avci, T., Ozturk, B., & Kilic, G. (2021). Assessment of the effect of marination with organic fruit vinegars on safety and quality of beef. International Journal of Food Microbiology, 336, 108904. https://doi.org/10.1016/j.ijfoodmicro.2020.108904
28. Siroli, L., Baldi, G., Soglia, F., Bukvicki, D., Patrignani, F., Petracci, M., & Lanciotti, R. (2020). Use of essential oils to increase the safety and the quality of marinated pork loin. Foods, 9(8), 987. https://doi.org/10.3390/foods9080987
29. Tkacz, K., Modzelewska-Kapituła, M., Petracci, M., & Zduńczyk, W. (2021). Improving the quality of sous-vide beef from Holstein-Friesian bulls by different marinades. Meat Science, 182, 108639. https://doi.org/10.1016/j.meatsci.2021.108639
30. Yi, J. J., Qu, H., Wu, Y. Z., Wang, Z. Y., & Wang, L. (2017). Study on antitumor, antioxidant and immunoregulatory activities of the purified polyphenols from pinecone of Pinus koraiensis on tumor-bearing S180 mice in vivo. International Journal of Biological Macromolecules, 94(Part A), 735–744. https://doi.org/10.1016/j.ijbiomac.2016.10.071
31. Zhang, Z., Xiao, H., Jiao, C. X., & Liu, G. M. (2010). Studies on chemical constituents from Pinus armandi Franch. Journal of Anhui Agricultural Sciences, 38(24), 13258–13259.
32. Wang, T., & Hong, M. (2016). Solid-state NMR investigations of cellulose structure and interactions with matrix polysaccharides in plant primary cell walls. Journal of Experimental Botany, 67(2), 503–514. https://doi.org/10.1093/jxb/erv416
33. Wang, Q., Buchanan, R. L., & Tikekar, R. V. (2019). Evaluation of adaptive response in E. coli O157: H7 to UV light and gallic acid based antimicrobial treatments. Food control, 106, 106723. https://doi.org/10.1016/j.foodcont.2019.106723