Protective Effect of Taurine Against Acrylamide-induced Oxidative Stress in Rats

Yıl 2018, Cilt: 11 Sayı: 4, 479 - 490, 15.12.2018

Sinan İnce , Ulaş Acaröz , Damla Arslan-acaröz , Nuray Varol , Zeki Gürler , İsmail Küçükkurt , Hasan Hüseyin Demirel Abdullah Eryavuz

https://doi.org/10.30607/kvj.466888

Öz

The aim of the
present study was to evaluate the protective effect of taurine on acrylamide-induced oxidative stress in rats.
Totally, 35 Wistar albino male rats were fed standard rodent diet and divided
into 5 equal groups.  I. Group: control group, II. Group: only 15 mg/kg acrylamide
treated by gastric gavage for 60 days.
III., IV., and V. group received 15 mg/kg acrylamide with taurine at dose of 50, 100 and 200 mg/kg/day by gastric
gavage. Treatment with acrylamide significantly increased malondialdehyde
levels and decreased glutathione levels in blood and tissues of rats.
Additionally, acrylamide treatment decreased superoxide dismutase and catalase
activities in erythrocyte and tissues of rats. However, treatment of taurine
inhibited acrylamide-induced oxidative stress, lipid peroxidation, and enhanced
the antioxidant enzymes activities. Besides, mRNA expression levels of IFN-γ,
IL-1β, TNF-α, and NFĸB in liver and brain of acrylamide exposed rats were
decreased with taurine treatment. Moreover, taurine exhibited protective action
against the acrylamide-induced histopathological changes in tissues. In conclusion, it was
determined that taurine in a dose-dependent
manner has a protective effect against acrylamide induced oxidative stress by
increasing antioxidant defense mechanism in rats.

Anahtar Kelimeler

Acrylamide, taurine, rat, oxidative stress, lipid peroxidation, cytokine

Sıçanlarda Akrilamid ile İndüklenen Oksidatif Strese Karşı Taurinin Koruyucu Etkisi

Öz

Bu çalışmada, sıçanlarda akrilamid ile oluşturulan oksidatif strese
karşı antioksidan ajan olan taurinin koruyucu etkisinin araştırılması
amaçlandı. Toplam 35 Wistar erkek sıçan standart rodent yemiyle beslendi ve 5
eşit gruba ayrıldı. I. Grup: kontrol grubu, II. Grup: sadece 15 mg/kg
akrilamid 60 gün boyunca gastrik gavaj yolu ile verildi. III., IV. ve V.
gruplara sırasıyla akrilamid (15 mg/kg) ve taurin 50, 100 ve 200 mg/kg/gün
olacak şekilde ağızdan gastrik gavaj ile verildi. Akrilamid uygulaması
sıçanların kan ve dokularında malondialdehid seviyelerinde önemli bir artışa ve
glutatyon seviyelerinde azalmaya neden oldu. Buna ilave olarak, akrilamid
uygulaması ile süperoksid dismutaz ve katalaz aktivitelerinin sıçan eritrosit
ve dokularında azaldığı belirlendi. Taurin uygulamasının akrilamid ile
indüklenen oksidatif stres ve lipid peroksidasyonu azaltarak vücudun antioksidan savunma
sistemini iyileştirdiği belirlendi. Bunun yanı sıra, taurin uygulaması ile akrilamid
verilen sıçanların karaciğer ve beyin dokularındaki IFN-γ, IL-1β, TNF-α ve NFĸB
mRNA ekspresyon düzeylerinin azaldığı gözlendi. Ayrıca, taurin
dokularda akrilamid ile indüklenen oksidatif stres sonucu oluşan histopatolojik
değişikliklere karşı koruyucu etki gösterdi. Sonuçta doza bağlı olarak taurinin
sıçanlarda antioksidan savunma mekanizmasını artırarak akrilamidle indüklenen oksidatif strese karşı koruyucu etki
gösterdiği belirlendi. 

Anahtar Kelimeler

Akrilamid, taurin, oksidatif stres, lipid peroksidasyon, sitokin, sıçan

Kaynakça

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