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Investigation of the Effect of Vitamin C and Vitamin E on Serum Protein Fractions in Rats with Experimental Fluorosis

Yıl 2021, Cilt 14, Sayı 2, 262 - 267, 30.06.2021
https://doi.org/10.30607/kvj.826435

Öz

The aim of the present study was to investigate the effects of vitamins C (vit C) and E (vit E) on the serum protein fractions in rats with experimentally-induced chronic fluorosis. Wistar-Albino rats (male) were used as material (8 groups, each containing 8 rats). The experimental groups were control, protection groups (daily 150 ppm NaF containing drinking water for 16 weeks as ad-libitum, and, on alternate Vit C (100 mg/kg), Vit E (300 mg/kg) and Vit C+ Vit E (100 mg/kg + 300 mg/kg) for 16 weeks) therapy groups (daily 150 ppm NaF containing drinking water for 16 weeks as ad-libitum, then normal drinking water as ad-libitum, Vit C (100 mg/kg), Vit E (300 mg/kg) and Vit C + Vit E (100 mg/kg + 300 mg/kg) on alternate for 4 weeks). The end of experimental period, blood samples were collected and serums were obtained. Serum protein fractions in blood samples were determined with cellulose-acetate electrophoresis. Alpha-1 was significantly decreased and beta globulins increased in the experimental fluorosis group. In the groups in which vitamin C, vitamin E and combine combination of vitamin C and vitamin E were given for prevention and treatment, it was observed that these changing values approach to the control group.

Kaynakça

  • Abdel-Wahab WM. Protective effect of thymoquinone on sodium fluoride-induced hepatotoxicity and oxidative stress in rats. J Basic Appl Zool.2013;66:263-270.
  • Çetin S, Usta A, Ekici P, Dede S, Yüksek V. Sarcoptes ovis ile enfekte koyunlarda serum protein fraksiyon profili. Atatürk Üniv Vet Bil Derg. 2020;15(1):70-75
  • Dincer Y, Akcay T, Ilkova H, Alademir Z, Ozbay G. DNA damage and antioxidant defense inperipheral leukocytes of patients with type 1 diabetes mellitus. Mutat Res. 2003;527:49-55.
  • Guan ZZ, Xiao KQ, Zeng, XY, Long YG, Cheng YH, Jiang SF. Changed cellularmembrane lipid composition and lipid peroxidation of kidney in rats with chronic fluorosis. Arch Toxicol. 2000;74:602-608.
  • Guo H, Kuang P, Luo Q, Cui H, Deng H, Liu H, Lu Y, Fang J, Zuo Z, Deng J, Li Y, Wang X, Zhao L. Effects of sodium fluoride on blood cellular and humoral immunity in mice. Oncotarget. 2017;8(49):85504-85515.
  • Güzel KGU, Kirzioğlu Z, Adiloğlu AK, Ertürk MSÖ. Effect of fluoride on salivary immunoglobulins and sialic acid. Rev Assoc Med Bras. 2017;63:320-323.
  • Karagül H, Altintas A, FidanciUR, Sel T. Klinik Biyokimya. 2000;161–164. Medisan, Ankara.
  • Karn SS, Narasimhacharya AV. Physiologic and metabolic benefits of formulated diets and Mangifera indicain fluoride toxicity. J Dietary Suppl. 2015;12.
  • Kasai K, Field JB. Discrimination of multiple forms of phosphoprotein phosphatase in bovine thyroid. Metabolism. 1983;32:296-307.
  • Kleinsasser NH, Weissacher H, Wallner BC, Kastenbauer ER, Harréus UA. Cytotoxicity and genotoxicity of fluorides in human mucosa and lymphocytes. Laryngorhinootologie. 2001;80(4):187-190.
  • Kuang P, Deng H, Cui H, Chen L, Fang J, Zuo Z, Deng J, Wang X, Zhao L. Sodium fluoride (NaF) causes toxic effects on splenic development in mice. Oncotarget. 2017;8(3):4703-4717.
  • Kubota K, Lee DH, Tsuchiya M, Young CS, Everett ET, Martinez-Mier EA, Snead ML, Nguyen L, Urano F, Bartlett JD. Fluoride induces endoplasmic reticulum stress in ameloblasts responsible for dental enamel formation. J Biol Chem. 2005;280(24):23194-23202.
  • Li Y, Schellhorn HE. New developments and novel therapeutic perspectives for vitamin C. J Nutr. 2007;137:2171–2184.
  • Loft S, Møller P, Cooke MS, Rozalski R, Olinski R. Antioxidant vitamins and cancer risk: is oxidative damage to DNA a relevant biomarker? Eur J Nutr. 2008;47:19-28.
  • Makarov SV, SpitsynVA, Kravchuk OI, Bychkovskaia LS. Qualitative and quantitative variation of serum proteins in fluorosis patients. Genetika. 1999;35(9):1305-1308.
  • Manojlović Z, Kopitar Z, Lenardic A, Plavsić F. Changes in plasma proteins and drug distribution in kidney and liver diseases. Lijecnicki Vjesnik. 1993;115:366–369.
  • Mehmetoğlu I. Clinical Biochemistry Manual. 2002;124–129. Konya: İnci Offset.
  • Mendoza-Schulz A, Solano-Agama C, Arreola-Mendoza L, Reyes-Marquez B, Barbier O, Del Razo LM. The effects of fuoride on cell migration, cell proliferation, and cell metabolism in GH4C1 pituitary tumour cells. Toxicol Lett. 2009;190:179-186.
  • Møller P, Viscovich M, Lykkesfeldt J, Loft S, Jensen A, Poulsen HE.Vitamin C supplementation decreases oxidative DNA damage in mononuclear blood cells of smokers. Eur J Nutr. 2004;43:267–274.
  • Murray RK. Plasma proteins and immunoglobulins. Chapter 50. In RK Murray, DK Granner, PA Mayes, VW Radwell (Eds.). Harper’s Illustrated Biochemistry. 2003;(26th ed., pp. 580–582). New York: McGraw Hill Co.
  • Onat T, Kaya E, Sozmen EY. Human Biochemistry, 2002;p.184-218. Palme Publishing, Ankara,
  • Öner A, Dede S, Yur F, Oner A. The effect of vitamin C and vitamin E on DNA damage, oxidative status, and some biochemical parameters in rats with experimental fluorosis. Fluoride. 2020;53(1 Pt 2):154-163.
  • Sharma S, Parashar P, Sharma S, Sharma KP. Ameliorating role of lycopene, tomato puree, and spirulina + tomato puree on the hematology offluoride-exposed swiss albino mice. J Diet Suppl. 2018;15(6):827-841.
  • Susheela AK, Bhatnagar M. Reversal of fluoride induced cell injury throughelimination of fluoride and consumption of dietrich in essential nutrients and antioxidants. Mol Cell Biochem. 2002;234/235:335–340.
  • Susheela AK, Jethanandani P. Serum haptoglobin and C-reactive protein in human skeletal fluorosis.Clin Biochem.1994;27(6):463-468.
  • Tian X, Xie J, Chen X, Dong N, Feng J, Gao Y, Tian F, Zhang W, Qiu Y, Niu R, Ren X, Yan X. Deregulation of autophagy is involved in nephrotoxicity of arsenite and fluoride exposure during gestation to puberty in rat offspring. Arch Toxicol. 2020;94(3):749-760.
  • Tóthová C, Mihajlovičová X, Nagy O. The Use of Serum Proteins in the Laboratory Diagnosis of Health Disorders in Ruminants. In book: Ruminants - The Husbandry, Economic and Health Aspects. DOI: 10.5772/intechopen.72154, 2017.
  • Wang AG, Xia T, Chu QL, Zhang M, Liu F, Chen XM, Yang KD. Effects of fluoride on lipide peroxidation, DNA damage and apoptosis in human embryo hepatocytes. Biomed Environ Sci. 2004;17:217-222.
  • Wei Y, Zeng B, Zhang H, Chen C, Wu Y, Wang N, Wu Y, Shen L. iTRAQ-based proteomics analysis of serum proteins in wistar rats treated with sodium fluoride: ınsight into the potential mechanism and candidate biomarkers of fluorosis. Int J Mol Sci. 2016;17(10):E1644.
  • Yuksek V, Dede S, Ceylan E. The electrophoretical determination of serum protein fractions in lycopene treated experimental diabetic rats. Cell Biochem Biophys. 2013;67(3):1283-1289.
  • Yur F, Mert N, Dede S, Deger Y, Ertekin A, Mert H,Yașar S, Doğan I, Işık A. Evaluation of serum lipoprotein andtissue antioxidant levels in sheep with fluorosis. Fluoride 2013;46:90-96.
  • Yüksek V, Ekici P, Dede S, Çetin S, Usta A. Profiles of serum protein fractions pre-treatment and post-treatment in lambs with pica disorder. Turk J Vet Res. 2019;3(2):67-71

Deneysel Olarak Florozis Oluşturulan Ratlarda Vitamin C ve Vitamin E'nin Serum Protein Fraksiyonları Üzerindeki Etkisinin Araştırılması

Yıl 2021, Cilt 14, Sayı 2, 262 - 267, 30.06.2021
https://doi.org/10.30607/kvj.826435

Öz

Bu çalışma, deneysel olarak kronik florozis oluşturulan ratlarda vitamin C (vit C) ve E (vit E)'nin serum protein fraksiyonları üzerindeki etkilerini araştırmak amacıyla planlandı. Materyal olarak Wistar-Albino ratlar kullanıldı (Her biri 8 rat içeren, 8 grup). Deneme grupları; kontrol grubu, deneysel florozis grubu, koruma grupları (16 hafta boyunca, içme suyu içeren günlük 150 ppm NaF içeren içme suyu/ad-libitum ile birlikte vit C (100 mg / kg), vit E (300 mg / kg) ve vit C + vit E (100 mg/kg + 300 mg/kg)) ve tedavi grupları (16 hafta boyunca 150 ppm NaF içeren içme suyu/ad-libitum verildikten sonra tedavi amacıyla 4 hafta vit C (100 mg/kg), vit E (300 mg / kg) ve vit C + vit E (100 mg/kg + 300 mg/kg)) olarak oluşturuldu. Deneme sonunda kan örnekleri toplandı ve serumlar alındı. Bu örneklerde serum protein fraksiyonları selüloz-asetat elektroforezi ile belirlendi. Deneysel florozis grubunda total protein yüzde gram açısından, alfa-1’in önemli oranda azaldığı ve beta globülinlerin arttığı tespit edildi. Korunma ve tedavi amacıyla vitamin C, vitamin E ve vitamin C+vitamin E birlikte verildiği gruplarda, değişen bu değerlerin kontrol grubuna yaklaştığı görüldü.

Kaynakça

  • Abdel-Wahab WM. Protective effect of thymoquinone on sodium fluoride-induced hepatotoxicity and oxidative stress in rats. J Basic Appl Zool.2013;66:263-270.
  • Çetin S, Usta A, Ekici P, Dede S, Yüksek V. Sarcoptes ovis ile enfekte koyunlarda serum protein fraksiyon profili. Atatürk Üniv Vet Bil Derg. 2020;15(1):70-75
  • Dincer Y, Akcay T, Ilkova H, Alademir Z, Ozbay G. DNA damage and antioxidant defense inperipheral leukocytes of patients with type 1 diabetes mellitus. Mutat Res. 2003;527:49-55.
  • Guan ZZ, Xiao KQ, Zeng, XY, Long YG, Cheng YH, Jiang SF. Changed cellularmembrane lipid composition and lipid peroxidation of kidney in rats with chronic fluorosis. Arch Toxicol. 2000;74:602-608.
  • Guo H, Kuang P, Luo Q, Cui H, Deng H, Liu H, Lu Y, Fang J, Zuo Z, Deng J, Li Y, Wang X, Zhao L. Effects of sodium fluoride on blood cellular and humoral immunity in mice. Oncotarget. 2017;8(49):85504-85515.
  • Güzel KGU, Kirzioğlu Z, Adiloğlu AK, Ertürk MSÖ. Effect of fluoride on salivary immunoglobulins and sialic acid. Rev Assoc Med Bras. 2017;63:320-323.
  • Karagül H, Altintas A, FidanciUR, Sel T. Klinik Biyokimya. 2000;161–164. Medisan, Ankara.
  • Karn SS, Narasimhacharya AV. Physiologic and metabolic benefits of formulated diets and Mangifera indicain fluoride toxicity. J Dietary Suppl. 2015;12.
  • Kasai K, Field JB. Discrimination of multiple forms of phosphoprotein phosphatase in bovine thyroid. Metabolism. 1983;32:296-307.
  • Kleinsasser NH, Weissacher H, Wallner BC, Kastenbauer ER, Harréus UA. Cytotoxicity and genotoxicity of fluorides in human mucosa and lymphocytes. Laryngorhinootologie. 2001;80(4):187-190.
  • Kuang P, Deng H, Cui H, Chen L, Fang J, Zuo Z, Deng J, Wang X, Zhao L. Sodium fluoride (NaF) causes toxic effects on splenic development in mice. Oncotarget. 2017;8(3):4703-4717.
  • Kubota K, Lee DH, Tsuchiya M, Young CS, Everett ET, Martinez-Mier EA, Snead ML, Nguyen L, Urano F, Bartlett JD. Fluoride induces endoplasmic reticulum stress in ameloblasts responsible for dental enamel formation. J Biol Chem. 2005;280(24):23194-23202.
  • Li Y, Schellhorn HE. New developments and novel therapeutic perspectives for vitamin C. J Nutr. 2007;137:2171–2184.
  • Loft S, Møller P, Cooke MS, Rozalski R, Olinski R. Antioxidant vitamins and cancer risk: is oxidative damage to DNA a relevant biomarker? Eur J Nutr. 2008;47:19-28.
  • Makarov SV, SpitsynVA, Kravchuk OI, Bychkovskaia LS. Qualitative and quantitative variation of serum proteins in fluorosis patients. Genetika. 1999;35(9):1305-1308.
  • Manojlović Z, Kopitar Z, Lenardic A, Plavsić F. Changes in plasma proteins and drug distribution in kidney and liver diseases. Lijecnicki Vjesnik. 1993;115:366–369.
  • Mehmetoğlu I. Clinical Biochemistry Manual. 2002;124–129. Konya: İnci Offset.
  • Mendoza-Schulz A, Solano-Agama C, Arreola-Mendoza L, Reyes-Marquez B, Barbier O, Del Razo LM. The effects of fuoride on cell migration, cell proliferation, and cell metabolism in GH4C1 pituitary tumour cells. Toxicol Lett. 2009;190:179-186.
  • Møller P, Viscovich M, Lykkesfeldt J, Loft S, Jensen A, Poulsen HE.Vitamin C supplementation decreases oxidative DNA damage in mononuclear blood cells of smokers. Eur J Nutr. 2004;43:267–274.
  • Murray RK. Plasma proteins and immunoglobulins. Chapter 50. In RK Murray, DK Granner, PA Mayes, VW Radwell (Eds.). Harper’s Illustrated Biochemistry. 2003;(26th ed., pp. 580–582). New York: McGraw Hill Co.
  • Onat T, Kaya E, Sozmen EY. Human Biochemistry, 2002;p.184-218. Palme Publishing, Ankara,
  • Öner A, Dede S, Yur F, Oner A. The effect of vitamin C and vitamin E on DNA damage, oxidative status, and some biochemical parameters in rats with experimental fluorosis. Fluoride. 2020;53(1 Pt 2):154-163.
  • Sharma S, Parashar P, Sharma S, Sharma KP. Ameliorating role of lycopene, tomato puree, and spirulina + tomato puree on the hematology offluoride-exposed swiss albino mice. J Diet Suppl. 2018;15(6):827-841.
  • Susheela AK, Bhatnagar M. Reversal of fluoride induced cell injury throughelimination of fluoride and consumption of dietrich in essential nutrients and antioxidants. Mol Cell Biochem. 2002;234/235:335–340.
  • Susheela AK, Jethanandani P. Serum haptoglobin and C-reactive protein in human skeletal fluorosis.Clin Biochem.1994;27(6):463-468.
  • Tian X, Xie J, Chen X, Dong N, Feng J, Gao Y, Tian F, Zhang W, Qiu Y, Niu R, Ren X, Yan X. Deregulation of autophagy is involved in nephrotoxicity of arsenite and fluoride exposure during gestation to puberty in rat offspring. Arch Toxicol. 2020;94(3):749-760.
  • Tóthová C, Mihajlovičová X, Nagy O. The Use of Serum Proteins in the Laboratory Diagnosis of Health Disorders in Ruminants. In book: Ruminants - The Husbandry, Economic and Health Aspects. DOI: 10.5772/intechopen.72154, 2017.
  • Wang AG, Xia T, Chu QL, Zhang M, Liu F, Chen XM, Yang KD. Effects of fluoride on lipide peroxidation, DNA damage and apoptosis in human embryo hepatocytes. Biomed Environ Sci. 2004;17:217-222.
  • Wei Y, Zeng B, Zhang H, Chen C, Wu Y, Wang N, Wu Y, Shen L. iTRAQ-based proteomics analysis of serum proteins in wistar rats treated with sodium fluoride: ınsight into the potential mechanism and candidate biomarkers of fluorosis. Int J Mol Sci. 2016;17(10):E1644.
  • Yuksek V, Dede S, Ceylan E. The electrophoretical determination of serum protein fractions in lycopene treated experimental diabetic rats. Cell Biochem Biophys. 2013;67(3):1283-1289.
  • Yur F, Mert N, Dede S, Deger Y, Ertekin A, Mert H,Yașar S, Doğan I, Işık A. Evaluation of serum lipoprotein andtissue antioxidant levels in sheep with fluorosis. Fluoride 2013;46:90-96.
  • Yüksek V, Ekici P, Dede S, Çetin S, Usta A. Profiles of serum protein fractions pre-treatment and post-treatment in lambs with pica disorder. Turk J Vet Res. 2019;3(2):67-71

Ayrıntılar

Birincil Dil Türkçe
Konular Veteriner Hekimlik
Bölüm ARAŞTIRMA MAKALESİ
Yazarlar

Semiha DEDE (Sorumlu Yazar)
Van Yüzüncü Yıl Üniversitesi
0000-0001-5744-6327
Türkiye


Ahmet Cihat ÖNER
Van Yüzüncü yıl üniversitesi
0000-0001-6614-4347
Türkiye


Veysel YÜKSEK
Van Yüzüncü Yıl Üniversitesi
0000-0001-7432-4989
Türkiye


Sedat ÇETİN
Van Yüzüncü Yıl Üniversitesi
0000-0002-6102-8571
Türkiye


Ayşe USTA
Van Yüzüncü Yıl Üniversitesi
0000-0002-5522-3469
Türkiye

Yayımlanma Tarihi 30 Haziran 2021
Yayınlandığı Sayı Yıl 2021, Cilt 14, Sayı 2

Kaynak Göster

Bibtex @araştırma makalesi { kvj826435, journal = {Kocatepe Veterinary Journal}, issn = {1308-1594}, eissn = {2147-6853}, address = {}, publisher = {Afyon Kocatepe Üniversitesi}, year = {2021}, volume = {14}, number = {2}, pages = {262 - 267}, doi = {10.30607/kvj.826435}, title = {Deneysel Olarak Florozis Oluşturulan Ratlarda Vitamin C ve Vitamin E'nin Serum Protein Fraksiyonları Üzerindeki Etkisinin Araştırılması}, key = {cite}, author = {Dede, Semiha and Öner, Ahmet Cihat and Yüksek, Veysel and Çetin, Sedat and Usta, Ayşe} }
APA Dede, S. , Öner, A. C. , Yüksek, V. , Çetin, S. & Usta, A. (2021). Deneysel Olarak Florozis Oluşturulan Ratlarda Vitamin C ve Vitamin E'nin Serum Protein Fraksiyonları Üzerindeki Etkisinin Araştırılması . Kocatepe Veterinary Journal , 14 (2) , 262-267 . DOI: 10.30607/kvj.826435
MLA Dede, S. , Öner, A. C. , Yüksek, V. , Çetin, S. , Usta, A. "Deneysel Olarak Florozis Oluşturulan Ratlarda Vitamin C ve Vitamin E'nin Serum Protein Fraksiyonları Üzerindeki Etkisinin Araştırılması" . Kocatepe Veterinary Journal 14 (2021 ): 262-267 <https://dergipark.org.tr/tr/pub/kvj/issue/61139/826435>
Chicago Dede, S. , Öner, A. C. , Yüksek, V. , Çetin, S. , Usta, A. "Deneysel Olarak Florozis Oluşturulan Ratlarda Vitamin C ve Vitamin E'nin Serum Protein Fraksiyonları Üzerindeki Etkisinin Araştırılması". Kocatepe Veterinary Journal 14 (2021 ): 262-267
RIS TY - JOUR T1 - Deneysel Olarak Florozis Oluşturulan Ratlarda Vitamin C ve Vitamin E'nin Serum Protein Fraksiyonları Üzerindeki Etkisinin Araştırılması AU - Semiha Dede , Ahmet Cihat Öner , Veysel Yüksek , Sedat Çetin , Ayşe Usta Y1 - 2021 PY - 2021 N1 - doi: 10.30607/kvj.826435 DO - 10.30607/kvj.826435 T2 - Kocatepe Veterinary Journal JF - Journal JO - JOR SP - 262 EP - 267 VL - 14 IS - 2 SN - 1308-1594-2147-6853 M3 - doi: 10.30607/kvj.826435 UR - https://doi.org/10.30607/kvj.826435 Y2 - 2021 ER -
EndNote %0 Kocatepe Veteriner Dergisi Deneysel Olarak Florozis Oluşturulan Ratlarda Vitamin C ve Vitamin E'nin Serum Protein Fraksiyonları Üzerindeki Etkisinin Araştırılması %A Semiha Dede , Ahmet Cihat Öner , Veysel Yüksek , Sedat Çetin , Ayşe Usta %T Deneysel Olarak Florozis Oluşturulan Ratlarda Vitamin C ve Vitamin E'nin Serum Protein Fraksiyonları Üzerindeki Etkisinin Araştırılması %D 2021 %J Kocatepe Veterinary Journal %P 1308-1594-2147-6853 %V 14 %N 2 %R doi: 10.30607/kvj.826435 %U 10.30607/kvj.826435
ISNAD Dede, Semiha , Öner, Ahmet Cihat , Yüksek, Veysel , Çetin, Sedat , Usta, Ayşe . "Deneysel Olarak Florozis Oluşturulan Ratlarda Vitamin C ve Vitamin E'nin Serum Protein Fraksiyonları Üzerindeki Etkisinin Araştırılması". Kocatepe Veterinary Journal 14 / 2 (Haziran 2021): 262-267 . https://doi.org/10.30607/kvj.826435
AMA Dede S. , Öner A. C. , Yüksek V. , Çetin S. , Usta A. Deneysel Olarak Florozis Oluşturulan Ratlarda Vitamin C ve Vitamin E'nin Serum Protein Fraksiyonları Üzerindeki Etkisinin Araştırılması. Kocatepe Veterinary Journal. 2021; 14(2): 262-267.
Vancouver Dede S. , Öner A. C. , Yüksek V. , Çetin S. , Usta A. Deneysel Olarak Florozis Oluşturulan Ratlarda Vitamin C ve Vitamin E'nin Serum Protein Fraksiyonları Üzerindeki Etkisinin Araştırılması. Kocatepe Veterinary Journal. 2021; 14(2): 262-267.
IEEE S. Dede , A. C. Öner , V. Yüksek , S. Çetin ve A. Usta , "Deneysel Olarak Florozis Oluşturulan Ratlarda Vitamin C ve Vitamin E'nin Serum Protein Fraksiyonları Üzerindeki Etkisinin Araştırılması", Kocatepe Veterinary Journal, c. 14, sayı. 2, ss. 262-267, Haz. 2021, doi:10.30607/kvj.826435

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