Effects of paclobutrazol-containing seed priming on seedling traits in wheat

Yıl 2025, Sayı: Advanced Online Publication, 683 - 691

Bekir Atar , Derya Güloğlu

https://doi.org/10.37908/mkutbd.1695001

Öz

Paclobutrazol is a powerful growth regulator used during the seedling stage to control plant growth and impart desired morphological characteristics. In this study, the effects of paclobutrazol-containing priming applications on the seedling stage of wheat were investigated. Seeds of three wheat cultivars were sown in a solution containing paclobutrazol (PBZ 30) at a dose of 30 mg L-1 for 12 hours. One month after sowing, the seedlings were removed and measured. Local varieties Sarıbuğday and Gökala produced larger seedlings in terms of all traits analysed, while Tosunbey modern wheat variety produced smaller seedling traits. Priming treatments (Hydropriming and PBZ 30) increased seedling root dry weight and chlorophyll content. PBZ 30 application caused a 25% decrease in seedling height compared to the control but increased root wet and dry weight and chlorophyll content. Hydropriming treatment generally increased the above-ground parts (especially seedling height, 8.3 cm). A positive correlation was found between chlorophyll b content and seedling height, number of leaves, seedling stem wet weight and seedling stem dry weight.

Anahtar Kelimeler

Wheat , Wheat seedling , Priming , Paclobutrazol

Paclobutrazol içerikli priming uygulamasının buğdayda fide özelliklerine etkileri

Öz

Fide döneminde bitki gelişimini kontrol etmek ve istenen morfolojik özellikleri kazandırmak amacıyla kullanılan paclobutrazol güçlü bir büyüme düzenleyicisidir. Çalışmada paclobutrazol içerikli priming uygulamasının buğdayda fide dönemi etkileri incelenmiştir. Üç buğday çeşidine ait tohumlar 30 mg L-1 dozunda paclobutrazol (PBZ 30) içerikli çözeltide 12 saat süreyle bekletilmiş, deneme alanına ekilmiştir. Ekimden bir ay sonra fideler sökülmüş ve ölçümleri yapılmıştır. Sarıbuğday ve Gökala yerel çeşitleri incelenen tüm özellikler bakımından daha büyük fideler oluştururken, Tosunbey modern buğday çeşiti daha küçük fide oluşturmuştur. Priming uygulamaları (Hidropriming ve PBZ 30) fide kök kuru ağırlığı ve klorofil içeriklerini arttırmıştır. PBZ 30 uygulaması kontrole göre fide boyunda yaklaşık %25 oranında azalmaya neden olurken, kök yaş ve kuru ağırlığı ile klorofil içerilerini arttırmıştır. Hidropriming uygulaması genel olarak toprak üstü aksamlarında (özellikle fide boyunda, 8.3 cm) artışlara neden olmuştur. Klorofil b içeriği ile fide boyu, yaprak sayısı, fide sap yaş ağırlığı ve fide sap kuru ağırlığı arasında pozitif korelasyon bulunmuştur.

Anahtar Kelimeler

Buğday , Fide dönemi , Priming , Paclobutrazol

Kaynakça

• Abbadi, A., Shekari, F., & Mustafavi, S.H. (2015). Effect of paclobutrazol and salicylic acid on antioxidants enzyme activity in drought stress in wheat. Idesia, 33 (4), 5-13.
• Ahmad, I., Kamran, M., Su, W., Haiqi, W., Ali, S., Bilegjargal, B., Ahmad, S., Liu, T., Cai, T., & Han, Q. (2019). Application of uniconazole improves photosynthetic efficiency of maize by enhancing the antioxidant defense mechanism and delaying leaf senescence in semiarid regions. Journal of Plant Growth Regulation, 38, 855-869.
• Albrecht, M.L. (1992). Tips on the use of chemical growth regulators on floriculture crops. In H. K. Tayama, Larson, A.R., Hammer, A.P., Roll, J.T. (Ed.), Plant regulators. Columbus, Ohio : Ohio Florists' Association.
• Antosovsky, J., & Ryant, P. (2015). The yield and quality of winter wheat (Triticum aestivum L.) grain after application of micronutrients on seed. Mendel. Net, 17-22.
• Arnon, D.I. (1949). Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24 (1), 1.
• Atar, B., Uygur, V., & Sukuşu, E. (2020). Effects of priming with copper, zinc and phosphorus on seed and seedling composition in wheat and barley. Türk Tarım ve Doğa Bilimleri Dergisi, 7 (1), 104-111.
• Bañón, S., González, A., Cano, E.A., Franco, J.A., & Fernández, J.A. (2002). Growth, development and colour response of potted Dianthus caryophyllus cv. Mondriaan to paclobutrazol treatment. Scientia horticulturae, 94 (3-4), 371-377.
• Berova, M., Zlatev, Z., & Stoeva, N. (2002). Effect of paclobutrazol on wheat seedlings under low temperature stress. Bulg. J. Plant Physiol, 28 (1-2), 75-84.
• Burrows, G., Boag, T., & Stewart, W. (1992). Changes in leaf, stem, and root anatomy of Chrysanthemum cv. Lillian Hoek following paclobutrazol application. Journal of Plant Growth Regulation, 11, 189-194.
• Crouch, I. (1990). The effect of seaweed concentrate on plant growth. [Doctoral dissertation, Department of Botany, Faculty of Science University of Natal].
• Davis, T.D., Steffens, G.L., & Sankhla, N. (1988). Triazole plant growth regulators. Horticultural Reviews, 10, 63-105.
• Di Benedetto, A., & Klasman, R. (2007). The effects of plug cell volume, paclobutrazol height control and the transplant stage on the post-transplant growth of Impatiens wallerana pot plant. European Journal of Horticultural Science, 72 (5), 193.
• Durgun, B., Uygur, V., Durgun, B., & Sukuşu, E. (2017). Isparta-Atabey ovası topraklarında mikro element yarayışlılığı ile toprak özellikleri arasındaki ilişkilerin temel bileşen analizi ile belirlenmesi. Anadolu Tarım Bilimleri Dergisi, 32 (2), 258-268.
• Fletcher, R.A., Gilley, A., Sankhla, N., & Davis, T.D. (2010). Triazoles as plant growth regulators and stress protectants. Horticultural Reviews, 24, 55-138.
• Gai, D., Liu, W., Liang, J., Guo, L., Geng, Y., Zhang, Q., Du, J., Gao, J., & Shao, X. (2023). The Effects of paclobutrazol seed soaking on biomass production and yield formation in direct-seeded rice. Agronomy, 13 (5), 1402.
• Gao, J., Hofstra, G., & Fletcher, R. (1988). Anatomical changes induced by triazoles in wheat seedlings. Canadian Journal of Botany, 66 (6), 1178-1185.
• Gill, D., & Singh, O. (1993). Modification of growth, yield and lodging response of lentil to phosphorus by paclobutrazol. Annals of Applied Biology, 123 (3), 667-671.
• Grossmann, K. (1990). Plant growth retardants as tools in physiological research. Physiologia Plantarum, 78 (4), 640-648.
• Jaleel, C.A., Gopi, R., Manivannan, P., & Panneerselvam, R. (2007). Responses of antioxidant defense system of Catharanthus roseus (L.) G. Don. to paclobutrazol treatment under salinity. Acta Physiologiae Plantarum, 29, 205-209.
• Kamran, M., Ahmad, I., Wu, X., Liu, T., Ding, R., & Han, Q. (2018). Application of paclobutrazol: a strategy for inducing lodging resistance of wheat through mediation of plant height, stem physical strength, and lignin biosynthesis. Environmental Science and Pollution Research, 25, 29366-29378.
• Karagüzel, O. (1999). Effects of paclobutrazol on growth and flowering of Bougainvillea spectabilis Willd. Turkish Journal of Agriculture and Forestry, 23 (8), 527-532.
• Khan, A.R., Ullah, I., Khan, A.L., Hong, S.-J., Waqas, M., Park, G.-S., Kwak, Y., Choi, J., Jung, B.-K., & Park, M. (2014). Phytostabilization and physicochemical responses of Korean ecotype Solanum nigrum L. to cadmium contamination. Water, Air, & Soil Pollution, 225, 1-11.
• Kraus, T.E., McKersie, B.D., & Fletcher, R.A. (1995). Paclobutrazol-induced tolerance of wheat leaves to paraquat may involve increased antioxidant enzyme activity. Journal of Plant Physiology, 145 (4), 570-576.
• Khayatnezhad, M., & Gholamin, R. (2012). The effect of drought stress on leaf chlorophyll content and stress resistance in maize cultivars (Zea mays). African Journal of Microbiology Research, 6 (12), 2844-2848.
• Kuai, J., Yang, Y., Sun, Y., Zhou, G., Zuo, Q., Wu, J., & Ling, X. (2015). Paclobutrazol increases canola seed yield by enhancing lodging and pod shatter resistance in Brassica napus L. Field Crops Research, 180, 10-20. Larson, R.A. (1985). Growth regulators in floriculture. Hortic. Rev, 7, 399-348.
• Liu, Y., Fang, Y., Huang, M., Jin, Y., Sun, J., Tao, X., Zhang, G., He, K., Zhao, Y., & Zhao, H. (2015). Uniconazole-induced starch accumulation in the bioenergy crop duckweed (Landoltia punctata) II: transcriptome alterations of pathways involved in carbohydrate metabolism and endogenous hormone crosstalk. Biotechnology for Biofuels, 8, 1-12.
• Mahmood, A., Turgay, O.C., Farooq, M., & Hayat, R. (2016). Seed biopriming with plant growth promoting rhizobacteria: a review. FEMS Microbiology Ecology, 92 (8), fiw112.
• Miller, A., & Armitage, A.M. (2002). Temperature, irradiance, photoperiod, and growth retardants influence greenhouse production of Angelonia angustifolia Benth. Angel Mist series. HortScience, 37 (2), 319-321.
• Miraj, G., Shah, H., & Arif, M. (2013). Priming maize (Zea mays) seed with phosphate solutions improves seedling growth and yield. J. Anim. Plant Sci., 23, 893-899.
• Pasian, C., & Bennett, M. (2001). Paclobutrazol soaked marigold, geranium, and tomato seeds produce short seedlings. HortScience, 36 (4), 721-723.
• Rajala, A., Peltonen-Sainio, P., Onnela, M., & Jackson, M. (2002). Effects of applying stem-shortening plant growth regulators to leaves on root elongation by seedlings of wheat, oat and barley: mediation by ethylene. Plant Growth Regulation, 38, 51-59.
• Rieger, M., & Scalabrelli, G. (1990). Paclobutrazol, root growth, hydraulic conductivity, and nutrient uptake of ‘Nemaguard’peach. HortScience, 25 (1), 95-98.
• Sharma, A., Kumar, V., Shahzad, B., Ramakrishnan, M., Singh Sidhu, G.P., Bali, A.S., ... & Zheng, B. (2020). Photosynthetic response of plants under different abiotic stresses: a review. Journal of Plant Growth Regulation, 39, 509-531.
• Singh, S., & Rao, D. (1981). Certain responses of wheat plants to cement dust pollution. Environmental Pollution Series A, Ecological and Biological, 24 (1), 75-81.
• Tripathi, S.C., Sayre, K., Kaul, J., & Narang, R. (2003). Growth and morphology of spring wheat (Triticum aestivum L.) culms and their association with lodging: effects of genotypes, N levels and ethephon. Field Crops Research, 84 (3), 271-290.
• Ucan, U., & Uğur, A. (2021). Acceleration of growth in tomato seedlings grown with growth retardant. Turkish Journal of Agriculture and Forestry, 45 (5), 669-679.
• Yılmaz, M.T. (2018). Effects of Cement dust on chlorophyll and metabolism products. Kastamonu University Journal of Forestry Faculty, 18 (3), 279-287.
• Zhang, M., Duan, L., Tian, X., He, Z., Li, J., Wang, B., & Li, Z. (2007). Uniconazole-induced tolerance of soybean to water deficit stress in relation to changes in photosynthesis, hormones and antioxidant system. Journal of Plant Physiology, 164 (6), 709-717.
• Zhang, Y., He, Z., Xing, P., Luo, H., Yan, Z., & Tang, X. (2024). Effects of paclobutrazol seed priming on seedling quality, photosynthesis, and physiological characteristics of fragrant rice. BMC Plant Biology, 24 (1), 53.