Rize’den Toplanan Altı Bitkinin İn Vitro Üreaz İnhibe Edici ve Antioksidan Aktivitesi

Yıl 2023, Cilt: 13 Sayı: 1, 98 - 106, 27.01.2023

Elif Dilmaç , Melike Sucu , Tuğba Günbatan , İlhan Gürbüz

https://doi.org/10.31020/mutftd.1184609

Öz

Giriş ve amaç: Üreaz enzimi, Helicobacter pylori nedenli peptik ülserin tedavisi için yeni ilaç adayı bileşiklerin hedeflerinden biri haline gelmiştir. Bu çalışmada Rize’den toplanan altı yabani bitkinin [Daphne pontica L., Lotus corniculatus L., Lythrum salicaria L., Medicago sativa L., Potentilla reptans L. ve Senecio leucanthemifolius subsp. vernalis (Waldst. & Kit.) Greuter] üreaz enzimini inhibe edici aktivitesinin belirlenmesi amaçlanmıştır. Bununla birlikte peptik ülserin önlenmesinde rol oynayan mekanizmalardan biri olan antioksidan aktiviteleri belirlenmiş, toplam fenol ve flavonoit içerikleri de tespit edilmiştir.
Yöntem: Bitkiler Rize Merkez ve İkizdere ilçesinden toplanıp %80’lik etanol ekstreleri hazırlanmış ve üreaz enzimini inhibe edici aktiviteleri belirlenmiştir. Hazırlanan ekstrelerin antioksidan aktivitesi DPPH, ABTS ve CUPRAC testleri ile tespit edilmiş, toplam fenol ve flavonoit miktar tayinleri yapılmıştır.
Bulgular: İn vitro testler sonucunda S. leucanthemifolius subsp. vernalis’in %80 etanol ekstresi üreaz enzimini düşük oranda inhibe ederken (%13,7) diğer çalışılan ekstrelerin daha kuvvetli (% 24,6 ve %31,3 arasında değişen oranlarda) inhibe ettiği görülmüştür. Çalışılan ekstrelerin genel olarak kuvvetli antioksidan aktiviteye sahip olduğu belirlenmiştir. En yüksek toplam fenol ve flavonoit içerikleri sırasıyla L. salicaria ve L. corniculatus’da tespit edilmiştir.
Sonuç: Çalışmaya dâhil edilen bitkilerin genel olarak üreaz enzimini belirli bir düzeyde inhibe ettiği belirlenmiştir. D. pontica, L. salicaria ve P. reptans yüksek antioksidan aktivite, toplam fenol ve flavonoit içerikleri ile öne çıkmıştır.

Anahtar Kelimeler

Üreaz, Daphne pontica, Lotus corniculatus, Lythrum salicaria, Medicago sativa, Potentilla reptans, Urease

In Vitro Urease Inhibitory and Antioxidant Activity of Six Plants Collected from Rize

Öz

Introduction: The urease enzyme has become one of the targets of new drug candidate compounds for the treatment of peptic ulcer caused by Helicobacter pylori. In this study, it was aimed to determine the urease enzyme inhibitory activity of six wildly grown plants [Daphne pontica L., Lotus corniculatus L., Lythrum salicaria L., Medicago sativa L., Potentilla reptans L. and Senecio leucanthemifolius subsp. vernalis (Waldst. & Kit.) Greuter] collected from Rize. In addition, their antioxidant activities, one of the mechanisms that play a role in the prevention of peptic ulcer, and total phenol and flavonoid contents were also determined.
Methods: Plants were collected in Rize Center and İkizdere district, 80% ethanol extracts were prepared from their aerial parts and their urease enzyme inhibitory activity was determined. The antioxidant activities of the prepared extracts were determined with DPPH, ABTS and CUPRAC tests, and their total phenol and flavonoid amounts were determined.
Results: As a result of in vitro tests, 80% ethanol extract of S. leucantemifolius subsp. vernalis was seen to inhibit urease enzyme at a low rate (13,7%); while other studied extracts moderately inhibited it more strongly (at rates varying between 24,6% and 31,3%). It was determined that studied extracts generally have strong antioxidant activity. The highest total phenol and flavonoid contents were detected in L. salicaria and L. corniculatus, respectively.
Conclusion: It was determined that the plants included in the study generally inhibited the urease enzyme at a certain level. D. pontica, L. salicaria and P. reptans stood out with their high antioxidant activity, total phenol and flavonoid contents. Although very strong urease inhibitory activity was not observed with extracts; compounds, that could be isolated by bioactivity guided fractionation studies or their derivatives, could possibly show strong urease inhibitory activity and new urease inhibitory compounds could be found.

Anahtar Kelimeler

Urease, Daphne pontica, Lotus corniculatus, Lythrum salicaria, Medicago sativa, Potentilla reptans

Kaynakça

• 1. Gürbüz ED, Yılmaz Ö. Helicobacter pylori’nin yaşam stratejisi. Türk Mikrobiyoloji Cemiyeti Dergisi 2011;41(2):49-56.
• 2. Yılmaz Ö, Okçu N. Helicobacter pylori ve gastrointestinal sistemle ilişkili hastalıklar. Atatürk Üniversitesi Tıp Dergisi 2006;38:13-7.
• 3. Korona-Glowniak I, ve ark. The in vitro activity of essential oils against Helicobacter pylori growth and urease activity. Molecules 2020;25(3).
• 4. Upadhyay LSB. Urease inhibitors: a review. Indian J Biotech 2012;11:381-8.
• 5. Svane S, ve ark. Inhibition of urease activity by diferent compounds provides insight into the modulation and association of bacterial nickel import and ureolysis. Sci Rep 2020;10:8503.
• 6. Matczuk D, Siczek A. Effectiveness of the use of urease inhibitors in agriculture: a review. Int Agrophys 2021;35:197-208.
• 7. Modolo LV, ve ark. An overview on the potential of natural products as ureases inhibitors: A review. J Adv Res 2015;6(1):35-44.
• 8. Tandon R, ve ark. Oxidative stress and antioxidants status in peptic ulcer and gastric carcinoma. Indian J Physiol Pharmacol 2004;48(1):115-8.
• 9. Ray PD, ve ark. Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cell Signal 2012;24(5):981-90.
• 10. Khan KM, ve ark. Synthesis and urease enzyme inhibitory effects of some dicoumarols. J Enzyme Inhib Med Chem 2004;19(4):367-71.
• 11. Matthaus B. Antioxidant activity of extracts obtained from residues of different oilseeds. J Agr Food Chem 2002;50:3444-52.
• 12. Apak R, ve ark. A novel total antioxidant capacity index for dietary polyphenols, vitamin C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J Agr Food Chem 2004;52:7970-81.
• 13. Gülçin İ. Comparison of in vitro antioxidant and antiradical activities of L-tyrosine and L-Dopa. Amino Acids 2007;32(3):431-8.
• 14. Woisky RG, Salatino A. Analysis of propolis: some parameters and procedures for chemical quality control. J Apicult Res 1998;37:99-105.
• 15. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viticult 1965;16:144-58.
• 16. López V, ve ark. Screening of Spanish medicinal plants for antioxidant and antifungal activities. Pharm Biol 2008;46(9):602-9.
• 17. Ilyasov IR, ve ark. ABTS/PP decolorization assay of antioxidant capacity reaction pathways. Int J Mol Sci 2020; 21(3).
• 18. Apak R, ve ark. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules 2007;12(7):1496-547.
• 19. Dawidowicz AL, Olszowy M. The importance of solvent type in estimating antioxidant properties of phenolic compounds by ABTS assay. Eur Food Res Technol 2013;236(6):1099-105.
• 20. Baytop T. Türkiye'de Bitkiler ile Tedavi. 2nd ed. İstanbul: Nobel Tıp Kitabevleri; 1999.
• 21. Miraldi E, Ferri S, Mostaghimi V. Botanical drugs and preparations in the traditional medicine of West Azerbaijan (Iran). J Ethnopharmacol 2001;75:77–87.
• 22. Tiţă I, Mogoşanu GD, Tiţă MG. Ethnobotanical inventory of medicinal plants from the south-west of Romania. Farmacia 2009;57(2):141-56.
• 23. Manayi A, ve ark. Biological activity and microscopic characterization of Lythrum salicaria L. DARU J Pharm Sci 2013;21:61.
• 24. Srećković N, ve ark. Lythrum salicaria L. (Lythraceae) as a promising source of phenolic compounds in the modulation of oxidative stress: Comparison between aerial parts and root extracts. Ind Crop Prod 2020;155.
• 25. Türker AU, ve ark. Evaluation of some traditional medicinal plants: phytochemical profile, antibacterial and antioxidant potentials. Romanian Biotechnol Lett 2021;26(2):2499-510.
• 26. Iancu IM. Phytochemical Evaluation and Cytotoxicity Assay of Lythri Herba Extracts. Farmacia 2021;69(1):51-8.
• 27. Vafi F, ve ark. Burn wound healing activity of Lythrum salicaria L. and Hypericum scabrum L. Wounds 2016;28(12):448-58.
• 28. Özkan AMG, Koyuncu M. Traditional medicinal plants used in Pınarbaşı area (Kayseri-Turkey). Turk J Pharm Sci 2005;2(2):63-83.
• 29. Gürbüz İ, ve ark. Anti-ulcerogenic activity of some plants used in folk medicine of Pınarbaşı (Kayseri, Turkey). J Ethnopharmacol 2005;101:313-8.
• 30. Uysal S, ve ark. Cytotoxic and enzyme inhibitory potential of two Potentilla species (P. speciosa L. and P. reptans Willd.) and their chemical composition. Front Pharmacol 2017;8:290.
• 31. Altundağ E, Öztürk M. Ethnomedicinal studies on the plant resources of east Anatolia, Turkey. Procedia Soc Behav Sci 2011;19:756-77.
• 32. Yerlikaya S, ve ark. Investigation of chemical profile, biological properties of Lotus corniculatus L. extracts and their apoptotic-autophagic effects on breast cancer cells. J Pharm Biomed Anal 2019;174:286-99.
• 33. Erarslan ZB, Kültür Ş. Ethnoveterinary medicine in Turkey: a comprehensive review. Turk J Vet Anim Sci 2019;43(5):555-82.
• 34. Bora KS, Sharma A. Phytochemical and pharmacological potential of Medicago sativa: a review. Pharm Biol 2011;49(2):211-20.