Determination of Antioxidant Activities of Water, Ethanol, Acetone, Chloroform and Hexane Extracts of Helichrysum plicatum Flowers

Abstract

Helichrysum plicatum is an annual flowering plant from the Asteraceae family and is widely used in the treatment of various ailments among the people. Helichrysum, which is generally known as " mantuvar, ölmez çiçek and altın otu " in Anatolia, is also used against colds, diabetes and stomach ailments in order to reduce kidney stones. In this study, antioxidant activities were investigated in flower extracts of Helichrysum plicatum prepared with solvents of different polarities (water, ethanol, acetone, chloroform, hexane). The antioxidant activities of the extracts at different concentrations (10-80 μg/ml) were determined by Copper (II) reduction (CUPRAC), ABTS (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)) and DPPH (1,1-Diphenyl 2-picryl hydrazil) methods. The results were evaluated by comparing them with BHT, BHA and Trolox standard antioxidant substances. It was determined that the antioxidant activities of the extracts of the plant obtained with different solvents were lower than the standard antioxidant substances. However, water extract in the CUPRAC and DPPH methods and ethanol extract in ABTS method showed higher antioxidant activity than other extracts. These results demonstrate that flower extracts of Helichrysum plicatum are available natural antioxidant sources for a variety of applications in the cosmetic, pharmaceutical and food industries.

Keywords

• Helichrysum plicatum
• Antioxidant activitiy
• CUPRAC
• ABTS
• DPPH

Helichrysum plicatum Çiçeklerinin Su, Etanol, Aseton, Kloroform ve Hekzan Ekstrelerinin Antioksidan Aktivitelerinin Belirlenmesi

Öz

Helichrysum plicatum Asteracae familyasından olup tek yıllık çiçekli bir bitki olup, halk arasında çeşitli rahatsızlıkların tedavisinde yaygın olarak kullanılmaktadır. Anadolu’da genel olarak “mantuvar, ölmez çiçek ve altın otu” olarak bilinen Helichrysum böbrek taşlarını düşürmek amacıyla, soğuk algınlığı, şeker hastalığı ve mide rahatsızlıklarına karşı da kullanılmaktadır. Bu çalışmada, Helichrysum plicatum’un çiçeklerinin farklı polariteye sahip çözücülerle (su, etanol, aseton, kloroform, hekzan) hazırlanmış ekstrelerinin antioksidan aktiviteleri araştırılmıştır. Ekstrelerin farklı konsantrasyonlardaki (10-80 μg/ml) antioksidan aktiviteleri, Bakır (II) indirgeme (CUPRAC), ABTS (2,2’-azinobis-(3-etilbenzotiazolin–6-sülfonik asit)) ve DPPH (1,1-Difenil 2-pikril hidrazil) yöntemleriyle belirlenmiş olup, sonuçlar BHT, BHA ve Troloks standart antioksidan maddelerle karşılaştırılarak değerlendirilmiştir. Bitkinin farklı çözücülerle elde edilen ekstrelerine ait antioksidan aktivitelerinin, standart antioksidan maddelerden daha düşük olduğu tespit edilmiştir. Bununla birlikte CUPRAC ve DPPH yöntemlerinde su ekstresi, ABTS yönteminde ise etanol ekstresi, diğer ekstrelerden daha yüksek antioksidan aktivite göstermiştir. Bu sonuçlar, Helichrysum plicatum’un çiçek özütlerinin kozmetik, farmasötik ve gıda endüstrilerinde çeşitli uygulamalar için ulaşılabilir doğal antioksidan kaynaklar olduğunu ortaya koymuştur.

Anahtar Kelimeler

• Helichrysum plicatum
• Antioksidan aktivite
• CUPRAC
• ABTS
• DPPH

Kaynakça

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