Saf Gerze tavuğu ve Sinop ili köy tavuğu popülasyonlarının sekiz polimorfik mikrosatellit lokusu bakımından karşılaştırılması

Öz

Araştırma, Sinop ili Gerze ilçesinde bulunan koruma altındaki saf Gerze tavuğu popülasyonu ve aynı ilde yetiştiriciliği yapılan köy tavuğu popülasyonları arasındaki genetik ilişkinin belirlenmesi amacıyla yürütülmüştür. Çalışmada yüksek düzeyde polimorfizm gösteren 8 adet mikrosatellit lokusu kullanılmıştır. Çalışmanın hayvan materyalini, Sinop ili Ayancık, Boyabat ve Gerze ilçelerinde bulunan 3 köy tavuğu popülasyonuna ait 60 örnek ve Gerze ilçesinde koruma altında tutulan 20 adet saf Gerze tavuğu oluşturmuştur. Sinop Gerze Köy, Sinop Gerze (Hacıkadı), Sinop Ayancık Köy ve Sinop Boyabat Köy popülasyonlarında beklenen heterozigotluk (H_e) değerleri sırasıyla 0.820, 0.755, 0.795 ve 0.741 olarak hesaplanmıştır. Popülasyon ayrımı yapılmaksızın hesaplanan akrabalı yetiştirme katsayısı (F_IS) değerleri LEI0166, MCW0034 ve MCW0069 lokuslarında -0.131, -0.195, -0.137 ve ADL0278, LEI0234, MCW0080, MCW0104, MCW0330 lokuslarında ise sırasıyla 0.178, 0.134, 0.766, 0.508 ve 0.433 olarak bulunmuştur. Temel koordinatlar ve Structure analizi sonuçları saf Gerze (Hacıkadı) popülasyonunun diğer köy popülasyonlarından genetik olarak farklı olduğunu ortaya koymuştur. Araştırmada, halk elinde yetiştiriciliği yapılan Gerze tavuklarının saf olmadığı, bu ırkın korunması için bölgede saf olarak yetiştirilmesinin gerekli olduğu sonucuna varılmıştır.

Anahtar Kelimeler

• Genetik çeşitlilik,Köy tavuğu,Mikrosatellitler,Sinop

Comparison of Gerze and village chicken populations in Sinop by eight polymorphic microsatellite loci

Öz

In this study, we aimed to identify the genetic relation between indigenous Gerze chicken population which is conserved in Gerze district of Sinop province and village chicken populations which are raised in Sinop. We used 8 highly polymorphic microsatellite loci for examinations. Animal materials of this study are total of 60 samples from 3 village chicken populations raised in Ayancık, Boyabat and Gerze districts, and 20 samples from Gerze chicken from the conserved population in Gerze district of Sinop province. Expected heterozygosity (H_e) values in Sinop Gerze Village, Sinop Gerze (Hacıkadı), Sinop Ayancık Village and Sinop Boyabat Village populations were calculated as 0.820, 0.755, 0.795 and 0.741, respectively. F_IS values were predicted as -0.131, -0.195, -0.137 in LEI0166, MCW0034 ve MCW0069 loci and 0.178, 0.134, 0.766, 0.508, 0.433 in ADL0278, LEI0234, MCW0080, MCW0104, MCW0330 loci respectively. Principal coordinate and Structure analyses’ results showed that Gerze (Hacıkadın) population is genetically different from the other village populations. In the study, it was concluded that the chickens in the Gerze villages are not pure breed. Thus, pure breed raising is necessary to conserve this breed in the region.

Anahtar Kelimeler

• Genetic diversity,Village chicken,Microsatellites,Sinop

Kaynakça

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