Kronik böbrek hastalığı olan hastalarda oksidatif stress düzeyi

Yıl 2018, Cilt: 10 Sayı: 1, 45 - 50, 05.04.2018

İhsan Ateş Nihal Özkayar Fatma Meriç Yılmaz Nergiz Bayrakçı Salim Neselioğlu Özcan Erel Ezgi Coşkun Yenigün Fatih Dede

https://doi.org/10.21601/ortadogutipdergisi.308443

Öz

Amaç: Bu çalışmada kronik böbrek hastalığı olan hastalarda
dinamik tiyol/disülfid dengesinin araştırılması amaçlandı.

Gereç
ve Yöntem:  Çalışmaya evre 1-5 (non diyaliz)
150 (57 erkek, 93 kadın) kronik böbrek
hastası ve (76 (30 erkek, 46 kadın) sağlıklı
kontrol grubu alındı. Kan tiyol/disülfid dengesi yeni geliştirilen otomatik ve
kalorimetrik bir yöntemle ölçüldü.

Bulgular: Tüm
popülasyonun serum thiol düzeyi ortalaması 320.8±57.8 µmol/L, total
thiol düzeyi ortalaması 352.0±61.5 µmol/L, ortalama disülfid düzeyi 15.6±5.3 µmol/L idi.
Kronik böbrek hastalarında ortalama serum thiol (p<0.001), total thiol (p<0.001) ve disülfid düzeyleri (p<0.001) kontrol grubuna kıyasla
anlamlı olarak daha düşüktü.  Kronik
böbrek hastaları ve kontrol grubu arasında disülfid/thiol, disülfid/total tihol
ve thiol /total thiol oranları açısından olarak anlamlı
farklılık saptanmadı.

Sonuç: Çalışamızda hem tiyol hem disülfid düzeyinde azalma
olması Kronik böbrek hastalarında serum tiyol düşüklüğünün
sadece tiyol gruplarının disülfide çevrimine değil, vücuttaki total tiyol
rezervinin azalmasına da bağlı olduğunu göstermektedir. Bu nedenle kronik böbrek hastalığında tiyol rezervini attırmaya yönelik nutrisyonel destek sağlanması
önerilebilir. 

Anahtar Kelimeler

: Merkaptanlar, oksidatif stres, sülfidril, tiyol oksidasyonu, üremi

Oxidative stress level in patients with chronic kidney disease

Öz

Background: In this study, we aimed to evaluate the presence of
oxidative stress in chronic kidney disease (CKD) by measuring dynamic thiol/disulphide homeostasis.

Material and Method: A
hundred fifty patients (57 men, 93 women) followed with a diagnosis of CKD
(stages 1-5, not on dialysis) and 76 healthy, disease-free individuals (30 men,
46 women) were included in the study. Blood thiol/disulphide homeostasis was
measured by newly developed automatic and colorimetric method by Erel and Neselioglu.

Results: The mean serum native thiol (p =
0.001), total thiol (p = 0.001) and disulphide (p =
0.041) levels were lower in CKD than in the control group. No significant
difference was found between the CKD and control groups in terms of disulphide/thiol, disulphide/total
thiol and thiol/total thiol ratios (p >
0.05). Serum native thiol and total thiol levels
were correlated negatively with both BMI and serum phosphorous level while both
the parameters were correlated positively with glomerular filtration rate,
total protein level and albumin level. A negative correlation was found between
total thiol and age. Disulphide
levels were correlated positively with albumin and total protein and negatively
with uric acid levels.

Conclusion: Our study shows that low serum thiol
levels in CKD is due to a decrease in total thiol reserve of the body and not
due to conversion of thiol groups into disulphides. 

Anahtar Kelimeler

Mercaptans, oxidative stress, sulfhydryl, thiol oxidation, uremia

Kaynakça

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