An Analysis of Plasma Thiol/Disulphide Homeostasis in Patients with Stable Angina

Abstract

Objective: To investigate thiol/disulfide homeostasis in patients with stable angina pectoris.

Materials and Methods: The study population consisted of 85 stable angina pectoris patients and 31 healthy volunteers. To compare the thiol/disulfide values of the patients and controls, the newly developed analysis method was used by Erel and Neselioglu. Disulfide/total thiol, free thiol/total thiol and disulfide/free thiol values were calculated for both groups.

Results: Disulfide, free thiol and total thiol values obtained in the study were; 18.00 µmol/L, 365.08 µmol/L, 401.10 µmol/L for the patient group and 9.67 µmol/L for the control group, 372.51 µmol/L, 391.85 µmol/L for the control group. It is the first time thiol/disulphide homeostasis in plasma was examined by new developed method in stable angina pectoris patients with this study. Disulfide, disulfide/free thiol and disulfide/total thiol values were significantly higher in the patient group compared to the control group. free thiol/total thiol levels were significantly lower in patients (p<0.001).

Conclusion: Thiol and disulfide values can be seen at high levels in patients with stable angina pectoris, so thiol/disulfide homeostasis may be an indicator for this disease.

Keywords

• Native thiol,total thiol,disulphide,thiol/disulphide,stable angina pectoris

Stabil Angina Pektoris Hastalarında Tiyol/Disülfid Homeostazı Analizi

Öz

Amaç: Stabil angina pektoris tanılı hastaların tiyol/disülfid homeostazını incelemektir.

Materyal ve Metot: Çalışma populasyonu 85 stabil angina pektoris hastası ile, 31 sağlıklı gönüllüden oluşmaktadır. Hasta ve kontrollerin tiyol/disülfid değerlerini karşılaştırmak için Erel ve Neşelioğlu tarafından yeni geliştirilen analiz metodu kullanılmıştır. Her iki grup için disülfid /total tiyol, serbest tiyol/total tiyol ve disülfid/serbest tiyol değerleri hesaplanmıştır. 

Bulgular: Çalışmada elde edilen disülfid, serbest tiyol ve total tiyol değerleri sırasıyla; hasta grubu için 18.00 µmol/L, 365,08 µmol/L, 401,10 µmol/L ve kontrol grubu için 9,67 µmol/L, 372,51 µmol/L, 391,85 µmol/L şeklinde bulunmuştur. Bu çalışma ile, stabil angina pektoris hastalarının tiyol/disulfid plazma değerleri yeni geliştirilen metot ile ilk defa belirlenmiştir. Disülfid, disülfid/serbest tiyol ve disülfid/total tiyol değerleri kontrole göre hasta grubunda anlamlı şekilde yüksek çıkarken; serbest tiyol/total tiyol değeri hastalarda anlamlı şekilde düşük çıkmıştır (p<0,001). 

Sonuç: Tiyol ve disülfit değerleri stabil anjina pektoris hastalarında yüksek düzeyde görülebilir, bu nedenle tiol/disülfid homeostazı bu hastalık için bir gösterge olabilir. 

Anahtar Kelimeler

• Serbest tiyol,total tiyol,tiyol/disülfid,disülfid,stabil angina

Kaynakça

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