Gender-specific effects of alternate-day fasting on body weight, oxidative stress, and metabolic health in middle-aged rats

Özgen Kılıç Erkek; Gülşah Gündoğdu

doi:10.31362/patd.1487708

EN TR

Gender-specific effects of alternate-day fasting on body weight, oxidative stress, and metabolic health in middle-aged rats

Öz

Purpose: The purpose of this study was to assess the effect of alternate-day fasting (ADF) concerning sex as well as its function in systemic and tissue-level oxidative stress alterations associated with aging. Materials and methods: Forty-two female (n=21) and male (n=21) Wistar rats (aged 16 months) were separated into six groups (n=7 each): Group-1 (control-male), Group-2 (1-month, ADF-male), Group-3 (2-month, ADFmale), Group-4 (control-female), Group-5 (1-month, ADF-female), and Group-6 (2-month, ADF-female). The ADF protocol was applied every other day for 24-h of fasting (three days/week). Serum samples were analyzed via ELISA to measure total oxidant-antioxidant status (TOS-TAS), and the oxidative stress index (OSI) was calculated. Results: 2-months of ADF treatment reduced body weight (BW) compared compliance control groups (p<0.001). All groups' cumulative food intake and retroperitoneal fat weight decreased with ADF (p<0.05). Both 1-month and 2-month ADF interventions had positive effects on reducing TOS and OSI in both liver and serum, with a significant decrease observed in both groups compared to their respective controls (p<0.001). The liver TAS significantly increased in female rats (p<0.05), but this increase did not reach a significant level in male rats. The difference in the serum TAS between the groups was not significant. Conclusions: This study evaluated the effects of ADF on BW, food consumption, and oxidative stress parameters in male and female rats. The findings highlight ADF's potential benefits in weight management and reducing oxidative stress. This study represents an important step in understanding the effects of ADF on metabolic health and in identifying potential clinical applications.

Anahtar Kelimeler

Aging, alternate-day fasting, food intake, gender, oxidative stress

Etik Beyan

Animal Experiments were approved by the Medical Ethics Committee of Pamukkale University (dated 15.12.2023 and numbered PAUHADYEK-2023/60758568-020-468703).

Orta yaş sıçanlarda gün aşırı açlık protokolünün vücut ağırlığı, oksidatif stres ve metabolik sağlık üzerine cinsiyete özgü etkileri

Öz

Amaç: Bu çalışmada, gün aşırı açlık protokolünün (ADF) cinsiyete özgü etkisinin ve yaşlanma ile ilişkili oksidatif stres değişimlerindeki işlevlerinin değerlendirilmesi amaçlandı. Gereç ve yöntem: Kırk iki dişi (n=21) ve erkek (n=21) 16 aylık Wistar sıçanları altı gruba (n=7) ayrıldı: Grup-1 (kontrol-erkek), Grup-2 (1 ay, ADF-erkek), Grup-3 (2 ay, ADF-erkek), Grup-4 (kontrol-dişi), Grup-5 (1 ay, ADFdişi) ve Grup-6 (2 ay, ADF-dişi). ADF protokolü günaşırı 24 saatlik oruç tutma şeklinde uygulandı (haftada üç gün). Serum örnekleri, ELISA yöntemiyle toplam oksidan-antioksidan seviyelerini (TOS-TAS) ölçmek için alındı ve oksidatif stres indeksi (OSI) hesaplandı. Bulgular: İki aylık-ADF tedavisinin kümültatif kontrol gruplarıyla karşılaştırıldığında vücut ağırlığında (VA) anlamlı azalma tespit edildi (p<0,001). Tüm gruplarda kümülatif gıda alımının ve retroperitoneal yağ ağırlığının ADF ile azaldığı görüldü (p<0,05). Hem 1 aylık hem de 2 aylık ADF uygulanması karaciğer ve serumda TOS seviyesi ve OSI'yi azaltmada olumlu etkiler gösterdi ve her iki grup da kendi kontrollerine göre anlamlı bir azalma gözlemlendi (p<0,001). Karaciğer TAS seviyesi dişi sıçanlarda anlamlı olarak arttı (p<0,05), ancak erkek sıçanlarda bu artış anlamlı bir seviyeye ulaşmadı. Gruplar arasındaki serum TAS seviyesinde anlamlı fark saptanmadı. Sonuç: Bu çalışma, erkek ve dişi sıçanlarda ADF'nin VA, gıda tüketimi ve oksidatif stres parametreleri üzerindeki etkilerini değerlendirdi. Bulgular, ADF'nin kilo yönetimi ve oksidatif stresi azaltmada potansiyel faydalarını vurgulamaktadır. Sonuç olarak, ADF'nin metabolik sağlık üzerindeki etkilerini anlamada ve olası klinik uygulamaları belirlemede önemli bir adımı temsil etmektedir.

Anahtar Kelimeler

Yaşlanma, alternatif günlerde açlık, gıda alımı, cinsiyet, oksidatif stres

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Tıbbi Fizyoloji (Diğer)

Bölüm

Araştırma Makalesi

Yazarlar

Özgen Kılıç Erkek
0000-0001-8037-099X
Türkiye

Gülşah Gündoğdu ^*
0000-0002-9924-5176
Türkiye

Erken Görünüm Tarihi

13 Haziran 2024

Yayımlanma Tarihi

9 Ekim 2024

Gönderilme Tarihi

21 Mayıs 2024

Kabul Tarihi

12 Haziran 2024

Yayımlandığı Sayı

Yıl 2024 Cilt: 17 Sayı: 4

DOI

https://doi.org/10.31362/patd.1487708

IZ

https://izlik.org/JA42AU44BA

APA

Kılıç Erkek, Ö., & Gündoğdu, G. (2024). Gender-specific effects of alternate-day fasting on body weight, oxidative stress, and metabolic health in middle-aged rats. Pamukkale Medical Journal, 17(4), 690-701. https://doi.org/10.31362/patd.1487708

AMA

1.Kılıç Erkek Ö, Gündoğdu G. Gender-specific effects of alternate-day fasting on body weight, oxidative stress, and metabolic health in middle-aged rats. Pam Tıp Derg. 2024;17(4):690-701. doi:10.31362/patd.1487708

Chicago

Kılıç Erkek, Özgen, ve Gülşah Gündoğdu. 2024. “Gender-specific effects of alternate-day fasting on body weight, oxidative stress, and metabolic health in middle-aged rats”. Pamukkale Medical Journal 17 (4): 690-701. https://doi.org/10.31362/patd.1487708.

EndNote

Kılıç Erkek Ö, Gündoğdu G (01 Ekim 2024) Gender-specific effects of alternate-day fasting on body weight, oxidative stress, and metabolic health in middle-aged rats. Pamukkale Medical Journal 17 4 690–701.

IEEE

[1]Ö. Kılıç Erkek ve G. Gündoğdu, “Gender-specific effects of alternate-day fasting on body weight, oxidative stress, and metabolic health in middle-aged rats”, Pam Tıp Derg, c. 17, sy 4, ss. 690–701, Eki. 2024, doi: 10.31362/patd.1487708.

ISNAD

Kılıç Erkek, Özgen - Gündoğdu, Gülşah. “Gender-specific effects of alternate-day fasting on body weight, oxidative stress, and metabolic health in middle-aged rats”. Pamukkale Medical Journal 17/4 (01 Ekim 2024): 690-701. https://doi.org/10.31362/patd.1487708.

JAMA

1.Kılıç Erkek Ö, Gündoğdu G. Gender-specific effects of alternate-day fasting on body weight, oxidative stress, and metabolic health in middle-aged rats. Pam Tıp Derg. 2024;17:690–701.

MLA

Kılıç Erkek, Özgen, ve Gülşah Gündoğdu. “Gender-specific effects of alternate-day fasting on body weight, oxidative stress, and metabolic health in middle-aged rats”. Pamukkale Medical Journal, c. 17, sy 4, Ekim 2024, ss. 690-01, doi:10.31362/patd.1487708.

Vancouver

1.Özgen Kılıç Erkek, Gülşah Gündoğdu. Gender-specific effects of alternate-day fasting on body weight, oxidative stress, and metabolic health in middle-aged rats. Pam Tıp Derg. 01 Ekim 2024;17(4):690-701. doi:10.31362/patd.1487708

Pamukkale Medical Journal Creative Commons Atıf-GayriTicari 4.0 Uluslararası Lisansı (CC BY NC) ile lisanslanmıştır.

Gender-specific effects of alternate-day fasting on body weight, oxidative stress, and metabolic health in middle-aged rats

Öz

Anahtar Kelimeler

Etik Beyan

Orta yaş sıçanlarda gün aşırı açlık protokolünün vücut ağırlığı, oksidatif stres ve metabolik sağlık üzerine cinsiyete özgü etkileri

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster