The effectiveness of antioxidant drugs such as N-acetylcysteine and Ozone on the stasis zone

Yıl 2021, , 474 - 480, 01.04.2021

Mustafa Atabey , Muhammed Raşid Aykota , Meriç Bostancı , Ayça Taş

https://doi.org/10.31362/patd.861244

Öz

Title: The effectiveness of antioxidant drugs such as N-acetylcysteine and Ozone on the stasis zone.
Short title: Effectiveness of N-acetylcysteine and Ozone on the stasis zone.
Purpose: Stasis zone, the zone around the coagulation zone, is important for the prevention necrosis in burns. Various drugs were studied in literature to prevent necrosis in stasis zone. The previous researches have shown the effectiveness of Nacetylcysteine treatment on both remote organs and local side in thermal injury. Demonstration of the effectiveness of N acetylcysteine and Ozone on stasis zone prevention in thermal injury was the goal of this study.
Methods: For this purpose, 24 4-month-old Wistar albino type female rats weighing 200±20 g were used in the study. Compound burning model was made in these rats. Next, the compound was applied to the combustion model to compare the effectiveness of N-acetylcysteine and Ozone. To identfy histopathological destruction severity a scoring method was applied. Malondialdhyede (MDA), Glutathione Peroxidase (GPx), Superoksid dismutase (SOD), and catalase (CAT) levels were analyzed in serum samples of the rats to find out the alterations in the balance between oxidatitaion and antioxidation system.
Results: While the highest MDA levels were observed in group I, the decrease was significant in group II and III (p=0.03 and p<0.02). SOD levels were significantly higher in group III compared to group II (p<0.05). CAT levels were higher in group II and III than group I (p<0.001, p<0.001). GPx levels were higher in group II (p<0.001), and group III (p<0.001). Histopathological scores were lower in group III than group I (p=0.001).
Conclusion: This study showed that both Ozone and N-acetylcysteine are effective agents in rescuing the stasis site. It was also concluded that Ozone might be more beneficial than N-acetylcysteine.

Anahtar Kelimeler

Burn, zone of stasis, antioxidan, ozone, oxidant

Destekleyen Kurum

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Proje Numarası

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N-asetilsistein ve ozon gibi antioksidan ilaçların staz zonu üzerindeki etkinliği

Öz

Amaç: Pıhtılaşma bölgesi etrafındaki bölge olan durağanlık bölgesi, yanıklarda nekrozun önlenmesi için önemlidir. Literatürde staz bölgesinde nekrozu önlemek için çeşitli ilaçlar çalışılmıştır. Önceki araştırmalar, termal yaralanmada N-asetilsistein tedavisinin hem uzak organlarda hem de lokal tarafta etkinliğini göstermiştir. Buna bağlı olarak, N-asetilsistein ve Ozonun termal yaralanma durağan bölgesi önleme etkinliğinde gösterilmesi amaçlanmıştır.
Yöntem: Bu amaçla çalışmada, ağırlıkları 200±20 g olan 24 adet 4 aylık Wistar albino tipi dişi sıçan kullanıldı. Bu sıçanlarda bileşik yanma modeli yapıldı. Sonra N-asetilsistein ve Ozonun etkililiğini karşılaştırmak için bileşik yanma modeline uygulandı. Histopatolojik yıkım şiddetini belirlemek için bir skorlama yöntemi uygulandı. Oksidasyon ve antioksidasyon sistemi arasındaki dengede meydana gelen değişiklikleri saptamak için sıçanların serum örneklerinde Malondialdehid (MDA), Glutatyon Perpxidaz (GPx), Superoksid dismutaz (SOD) ve katalaz (CAT) seviyeleri analiz edildi.
Bulgular: SOD düzeyleri grup III'te grup II'ye göre anlamlı olarak yüksekti (p<0,05). CAT seviyeleri grup II ve III'te grup I'den daha yüksekti (p<0,001, p<0,001). GPx düzeyleri grup II (p<0,001) ve grup III (p<0,001)'de daha yüksekti. Histopatolojik skorlar Grup III'de grup I'den daha düşüktü (p=0,001).
Sonuç: Bu çalışmada, hem Ozon hem de N-asetilsisteinin, staz bölgesinin kurtarılmasında etkili ajanlar olduğunu göstermiştir. Ayrıca Ozon, N-asetilsisteinden daha faydalı olabileceği sonucuna varılmıştır.

Anahtar Kelimeler

Yanık, staz bölgesi, antioksidan, ozon, oksidan.

Proje Numarası

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Kaynakça

• 1. Parihar A, Parihar M. S, Milner S, and Bhat S. Oxidative stress and anti-oxidative mobilization in burn injury Burns. 2008; 34: 6-17.
• 2. Jackson D. M. The diagnosis of the depth of burning. Brit j of surg 1953; 40: 588-596.
• 3. Tunali T, Sener G, Yarat A and Emekli N. Melatonin reduces oxidative damage to skin and normalizes blood coagulation in a rat model of thermal injury. Life sci 2005; 76: 1259-1265.
• 4. Vorauer-Uhl K, Fu¨ rnchlief E, Wagner A, Ferko B, Katinger H. Reepithelialization of experimental scalds effected by topically applied superoxide dismutase: controlled animal studies. Wound Repair Regen 2002; 10: 366–371.
• 5. Kaufman T, Neuman R. A and Weinberg A. Is post burn dermal ischemia enhanced by oxygen free radicals? Burns 1989; 15: 291-294.
• 6. Choi M, and Ehrlich H.P. U75412E, a lazaroid, prevents progressive burn ischemia in a rat burn model. The Amer j of path 1993; 142: 519.
• 7. Işik S, Şahin Ü, Ilgan S, Güler M, Günalp B and Selmanpakoǧlu N. Saving the zone of stasis in burns with recombinant tissue-type plasminogen activator (r-tPA): an experimental study in rats. Burns 1998; 24: 217-223.
• 8. Deniz M, Borman H, Seyhan T and Haberal M. An effective antioxidant drug on prevention of the necrosis of zone of stasis: N-acetylcysteine. Burns 2013; 39: 320-325.
• 9. Nguyen T. T, Cox C. S, Traber D. L, Gasser H, Redl H, Schlag G and Herndon D. N. Free radical activity and loss of plasma antioxidants, vitamin E, and sulfhydryl groups in patients with burns: the 1993 Moyer Award. The J of burn care & rehabil 1993; 14: 602-609.
• 10. Deniz M, Borman H, Seyhan T and Haberal M. An effective antioxidant drug on prevention of the necrosis of zone of stasis: N-acetylcysteine. Burns 2013; 39: 320-325.
• 11. Ritter C, Andrades M. E, Reinke A, Menna-Barreto S, Moreira J. C. F and Dal-Pizzol F. Treatment with N-acetylcysteine plus deferoxamine protects rats against oxidative stress and improves survival in sepsis. Critl care med. 2004; 32: 342-349.
• 12. Gürer A, Ozdoğan M, Gökakin A. K, Gömceli İ, Gülbahar O, Arikök A. T, ... and Aydin R. Tissue oxidative stress level and remote organ injury in two-hit trauma model of sequential burn injury and peritoneal sepsis are attenuated with N-acetylcysteine treatment in rats. Turk j of trau & emerg surg TJTES 2009; 15: 1-6.
• 13. Travagli V, Zanardi I, Valacchi G and Bocci V. Ozone and ozonated oils in skin diseases: a review. Mediat of infl 2010; 2010.
• 14. Zeng J and Lu J. Mechanisms of action involved in ozone-therapy in skin diseases. Inter immunopharma 2018; 56: 235-241.
• 15. Thérond P, Bonnefont-Rousselot D, Davit-Spraul A, Conti M and Legrand A. Biomarkers of oxidative stress: an analytical approach. Curr Opini in Cli Nutr & Metab Care 2000; 3: 373-384.
• 16. Gökakın A. K, Atabey M, Deveci K, Sancakdar E, Tuzcu M, Duger C and Topcu O. The effects of sildenafil in liver and kidney injury in a rat model of severe scald burn: a biochemical and histopathological study. Ulus Trav Acil Cer Derg 2014; 20: 319-327.
• 17. Regas F. C and Ehrlich H. P. Elucidating the vascular response to burns with a new rat model. The J of trau 1992; 32: 557-563.
• 18. Stevenson J. M, Gamelli R. L and Shankar R. A mouse model of burn wounding and sepsis. Humana Press, Totowa, NJ. In Wound Heal 2003; 95-105.
• 19. Nishikimi M, Rao N. A and Yagi K. The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem and biophyl res commun 1972; 46: 849-854.
• 20. Aebi H. Catalase in vitro. In Meth in enzym 1984; 105: 121-126.
• 21. Paglia D. E and Valentine W. N. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. The J of lab and cli med. 1967; 70: 158-169.
• 22. Singer A. J, McClain S. A, Taira B. R, Guerriero J. L and Zong W. Apoptosis and necrosis in the ischemic zone adjacent to third degree burns. Acade Emergy Med 2008; 15: 549-554.
• 23. Godar D. E. Preprogrammed and programmed cell death mechanisms of apoptosis: UV‐induced immediate and delayed apoptosis. Photochem and photobiol 1996; 63: 825-830.
• 24. Lubos E, Loscalzo J and Handy D. E. Glutathione peroxidase-1 in health and disease: from molecular mechanisms to therapeutic opportunities. Antioxi & redox signal 2011; 15; 1957-1997.