Yıl 2020, Cilt 13 , Sayı 3, Sayfalar 613 - 619 2020-09-18

Beta adrenergic receptors play role in the vasoconstrictor effect of dobutamine in the isolated rat aorta
İzole sıçan aortunda dobutaminin vazokonstriktör etkisinde beta adrenerjik reseptörler rol oynar

Hande Ozge ALTUNKAYNAK-CAMCA [1]


Purpose: Experimental evidence exists that cardiac action of dobutamine is mediated by alfa-1 and beta- adrenergic receptors. However, uncertainty remains regarding the vascular effect of dobutamine and contribution of beta-adrenergic receptors to this effect. The aim of the present study was to investigate the direct effect of dobutamine in the rat aorta and the role of beta-adrenergic receptors in this effect. Materials and methods: The isolated thoracic aortic rings were mounted in organ bath containing Krebs-Henseleit solution. After an equilibiration period, endothelial integrity was then checked by the response to acetylcholine (10 μM) in aortic rings pre-contracted with phenylephrine (1 μM). After washout, dobutamine (0.001-10 μM) was added to generate cumulative concentration–response curves (CCRCs). To investigate the role of alfa- and beta- adrenergic receptors in the dobutamine-induced vascular response, prazosin (0.0003 µM) or propranolol (1 µM) was added to the bath medium 30 min before the addition of dobutamine in some experiments.. Results: Dobutamine produced concentration-dependent contraction in the endothelium-intact isolated rat aorta. This effect was significantly inhibited by either propranolol or prazosin (p<0.05). Prazosin also significantly supressed the maximum vascular response obtained by dobutamine (p<0.05). Conclusion: The results, to the best of my knowledge, demonstrates for the first time that beta-adrenergic receptors are involved in the vasoconstrictor effect of dobutamine in the endothelium-intact rat aorta.
Amaç: Alfa-1 ve beta-adrenerjik reseptörlerin, dobutaminin kardiyak etkisine aracılık ettiğine ilişkin deneysel kanıt mevcuttur. Ancak, dobutaminin vasküler etkisi ve bu etkiye beta-adrenerjik reseptörlerin katkısı belirsizliğini korumaktadır. Bu çalışma, dobutaminin sıçan aortundaki doğrudan etkisini ve bu etkide beta adrenerjik reseptörlerin rolünü araştırmayı amaçlamıştır. Gereç ve yöntem: İzole edilmiş torasik aort halkaları Krebs-Henseleit solüsyonu içeren organ banyosuna asıldı. Dinlenme periyodu sonrası, aort halkaları fenilefrinle (1 μM) kasıldıktan sonra verilen asetilkolin (10 μM) yanıtıyla endotelyal bütünlük kontrol edildi. Yıkama sonrası, dobutamin (0.001-10 μM), kümülatif konsantrasyon-yanıt eğrilerini elde etmek için organ banyosuna eklendi. Dobutaminin oluşturduğu vasküler yanıtta alfa- ve beta-adrenerjik reseptörlerin rolünü araştırmak için, bazı deneylerde, prazosin (0.0003 µM) veya propranolol (1 µM) banyo ortamına dobutaminden 30 dakika önce eklendi. Bulgular: Dobutamin, endoteli sağlam izole sıçan aortunda konsantrasyon-bağımlı kontraksiyona neden oldu. Bu etki, propranolol veya prazosin varlığında anlamlı olarak inhibe edildi (p<0.05). Ayrıca, prazosin dobutaminle elde edilen maksimum vasküler yanıtı da baskıladı (p<0.05). Sonuç: Bu sonuçlar, bildiğim kadarıyla, ilk defa endoteli sağlam izole sıçan aortunda dobutaminin vazokonstriktör etkisinde beta-adrenerjik reseptörlerin ilişkisi olduğunu göstermektedir.
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Birincil Dil en
Konular Genel ve Dahili Tıp
Yayınlanma Tarihi Eylül 2020
Bölüm Araştırma Makalesi
Yazarlar

Orcid: 0000-0002-4547-7756
Yazar: Hande Ozge ALTUNKAYNAK-CAMCA (Sorumlu Yazar)
Kurum: UNIVERSITY OF HEALTH SCIENCES, GÜLHANE FACULTY OF PHARMACY
Ülke: Turkey


Teşekkür I would like to thank to Zeynep Elif YEŞİLYURT (PhD student at Ankara University Faculty of Pharmacy Department of Pharmacology) for technical support.
Tarihler

Yayımlanma Tarihi : 18 Eylül 2020

Bibtex @araştırma makalesi { patd700611, journal = {Pamukkale Tıp Dergisi}, issn = {}, eissn = {1308-0865}, address = {Pamukkale Üniversitesi Tıp Fakültesi Eğitim Blokları Kınıklı kampüsü 20070 Kınıklı, Denizli}, publisher = {Pamukkale Üniversitesi}, year = {2020}, volume = {13}, pages = {613 - 619}, doi = {10.31362/patd.700611}, title = {Beta adrenergic receptors play role in the vasoconstrictor effect of dobutamine in the isolated rat aorta}, key = {cite}, author = {Altunkaynak-camca, Hande Ozge} }
APA Altunkaynak-camca, H . (2020). Beta adrenergic receptors play role in the vasoconstrictor effect of dobutamine in the isolated rat aorta . Pamukkale Tıp Dergisi , 13 (3) , 613-619 . DOI: 10.31362/patd.700611
MLA Altunkaynak-camca, H . "Beta adrenergic receptors play role in the vasoconstrictor effect of dobutamine in the isolated rat aorta" . Pamukkale Tıp Dergisi 13 (2020 ): 613-619 <https://dergipark.org.tr/tr/pub/patd/issue/55097/700611>
Chicago Altunkaynak-camca, H . "Beta adrenergic receptors play role in the vasoconstrictor effect of dobutamine in the isolated rat aorta". Pamukkale Tıp Dergisi 13 (2020 ): 613-619
RIS TY - JOUR T1 - Beta adrenergic receptors play role in the vasoconstrictor effect of dobutamine in the isolated rat aorta AU - Hande Ozge Altunkaynak-camca Y1 - 2020 PY - 2020 N1 - doi: 10.31362/patd.700611 DO - 10.31362/patd.700611 T2 - Pamukkale Tıp Dergisi JF - Journal JO - JOR SP - 613 EP - 619 VL - 13 IS - 3 SN - -1308-0865 M3 - doi: 10.31362/patd.700611 UR - https://doi.org/10.31362/patd.700611 Y2 - 2020 ER -
EndNote %0 Pamukkale Tıp Dergisi Beta adrenergic receptors play role in the vasoconstrictor effect of dobutamine in the isolated rat aorta %A Hande Ozge Altunkaynak-camca %T Beta adrenergic receptors play role in the vasoconstrictor effect of dobutamine in the isolated rat aorta %D 2020 %J Pamukkale Tıp Dergisi %P -1308-0865 %V 13 %N 3 %R doi: 10.31362/patd.700611 %U 10.31362/patd.700611
ISNAD Altunkaynak-camca, Hande Ozge . "Beta adrenergic receptors play role in the vasoconstrictor effect of dobutamine in the isolated rat aorta". Pamukkale Tıp Dergisi 13 / 3 (Eylül 2020): 613-619 . https://doi.org/10.31362/patd.700611
AMA Altunkaynak-camca H . Beta adrenergic receptors play role in the vasoconstrictor effect of dobutamine in the isolated rat aorta. Pamukkale Tıp Dergisi. 2020; 13(3): 613-619.
Vancouver Altunkaynak-camca H . Beta adrenergic receptors play role in the vasoconstrictor effect of dobutamine in the isolated rat aorta. Pamukkale Tıp Dergisi. 2020; 13(3): 613-619.