Yıl 2021, Cilt 14 , Sayı 2, Sayfalar 354 - 360 2021-04-01

Periferik sinir üzerinde topikal uygulanan vancomisin toz perinöral fibrozise neden olur mu?
Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?

Semih Kivanc OLGUNER [1] , Mustafa ÇELİKTAŞ [2] , Kivilcim ERDOGAN [3] , Kenan DAĞLIOĞLU [4] , İsmail İŞTEMEN [5] , Ali ARSLAN [6] , Vedat AÇIK [7] , Ali ÖKTEN [8] , Yurdal GEZERCAN [9]


Amaç : Bu deneysel sıçan çalışmasının amacı direk olarak siyatik sinir üzerine topikal uygulanan vancomisin tozun perinöral fibrozis ve kronik inflamasyon gelişimine neden olup olmadığını araştırmaktır. Materyal-metod : Otuz adet erkek Wistar-Albino cins sıçan çalışmaya dahil edilerek intraperitoneal anestezi sonrası uygulanacak doz miktarına göre 10’arlı 3 gruba ayrıldı. Siyatik sinir ekplorasyonu ardından tek doz vankomisin toz verilen 10 sıçan (grup 1), çift doz vankomisin uygulanan 10 sıçan (grup 2) , 10 hayvana ise ilaç verilmeyerek kontrol grubu (grup 3) oluşturuldu. 3 hafta sonra ise sıçanlar sakrifiye edilerek siyatik sinir etrafındaki doku örnekleri alındı ve histopatolojik incelemeye gönderildi. Bulgular : Grup 1 de 1 (%10) hayvanda evre 1 perinöral fibrosis tespit edildi. Diğer 9 (%90) deney hayvanında epidural fibrozis görülmedi. Grup 2 deki 10 deney hayvanından 1 tanesinde (%10) evre 2, 1 tanesinde ise (%10) evre 1 perinöral fibrozis saptandı. Sekiz hayvanda (%80) ise perinöral fibrozis bulgusu saptanmadı. Grup 3 de ise 1 (%10) deney hayvanında evre 1 perinöral fibrozis saptandı. Kronik inflamasyon ise hiçbir deney hayvanında tespit edilmedi. İstatistiksel incelemeler sonucunda 3 grup arasında perinöral fibrozis açısından anlamlı farklılık saptanmadı (p=0.753). Sonuç : Bu deneysel hayvan çalışmasında vankomisin toz uygulanan hastalarda fibrozis gelişiminin gruplar arasında anlamlı farklılık göstermediğini saptadık. Daha büyük katılımlı ve histopatolojik incelemelerin daha detaylı yapılacağı deneysel çalışmalar sonucunda daha objektif sonuçlara varılacağı kanaatindeyiz
Purpose: The aim of this experimental rat study is to investigate whether vancomycin powder directly and topically applied on the sciatic nerve causes perineural fibrosis and chronic inflammation development. Materials and Methods: Thirty young adult male Wistar-Albino rats were included in the study and divided into 3 groups of 10 rats based on the dose to be applied after intraperitoneal anesthesia. After the sciatic nerve exploration, 10 rats (group 1) were administered a single dose of vancomycin powder, 10 rats (group 2) were administered a double dose of vancomycin, and control group was created with 10 animals which were administered no medication (group 3). After 3 weeks, rats were sacrificed, tissue samples were taken around sciatic nerve and sent for histopathological examination. Results: Grade 1 perineural fibrosis was detected in 1 animal (10%) in group 1. Grade 2 perineural fibrosis was detected in 1 animal (10%) and grade 1 perineural fibrosis was detected in 1 of 10 animals (10%) in group 2. In Group 3, grade 1 perineural fibrosis was detected in 1 experimental animal (10%). Chronic inflammation was not detected in any experimental animal. No significant difference was found between the 3 groups in terms of perineural fibrosis (p=0.753). Conclusion: In this experimental animal study, we found that fibrosis development did not differ significantly among the groups. We believe that more objective results can be obtained as a result of experimental studies with larger participation and more detailed histopathological examinations.
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Birincil Dil en
Konular Cerrahi
Yayınlanma Tarihi Nisan 2021
Bölüm Araştırma Makalesi
Yazarlar

Orcid: 0000-0002-5314-4636
Yazar: Semih Kivanc OLGUNER (Sorumlu Yazar)
Kurum: Adana City Training Research Hospital
Ülke: Turkey


Orcid: 0000-0003-2084-9725
Yazar: Mustafa ÇELİKTAŞ
Kurum: Ortopedia Hospital ADANA
Ülke: Turkey


Orcid: 0000-0002-4951-8703
Yazar: Kivilcim ERDOGAN
Kurum: Department of Pathology, Cukurova University School of Medicine, Adana, Turkey
Ülke: Turkey


Orcid: 0000-0003-3140-2937
Yazar: Kenan DAĞLIOĞLU
Kurum: Ahi Evran University, Department of Microbiology, Kırşehir
Ülke: Turkey


Orcid: 0000-0002-2341-4818
Yazar: İsmail İŞTEMEN
Kurum: Adana City Training Research Hospital, Adana
Ülke: Turkey


Orcid: 0000-0002-7457-5283
Yazar: Ali ARSLAN
Kurum: Adana City Training Research Hospital, Adana
Ülke: Turkey


Orcid: 0000-0002-0371-5883
Yazar: Vedat AÇIK
Kurum: Adana City Training Research Hospital, Adana
Ülke: Turkey


Orcid: 0000-0003-0292-201X
Yazar: Ali ÖKTEN
Kurum: Adana City Training Research Hospital, Adana
Ülke: Turkey


Orcid: 0000-0002-4124-2036
Yazar: Yurdal GEZERCAN
Kurum: Adana City Training Research Hospital, Adana
Ülke: Turkey


Tarihler

Yayımlanma Tarihi : 1 Nisan 2021

Bibtex @araştırma makalesi { patd789163, journal = {Pamukkale Tıp Dergisi}, issn = {}, eissn = {1308-0865}, address = {Pamukkale Üniversitesi Tıp Fakültesi Eğitim Blokları Kınıklı kampüsü 20070 Kınıklı, Denizli}, publisher = {Pamukkale Üniversitesi}, year = {2021}, volume = {14}, pages = {354 - 360}, doi = {10.31362/patd.789163}, title = {Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?}, key = {cite}, author = {Olguner, Semih Kivanc and Çeliktaş, Mustafa and Erdogan, Kivilcim and Dağlıoğlu, Kenan and İştemen, İsmail and Arslan, Ali and Açık, Vedat and Ökten, Ali and Gezercan, Yurdal} }
APA Olguner, S , Çeliktaş, M , Erdogan, K , Dağlıoğlu, K , İştemen, İ , Arslan, A , Açık, V , Ökten, A , Gezercan, Y . (2021). Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis? . Pamukkale Tıp Dergisi , 14 (2) , 354-360 . DOI: 10.31362/patd.789163
MLA Olguner, S , Çeliktaş, M , Erdogan, K , Dağlıoğlu, K , İştemen, İ , Arslan, A , Açık, V , Ökten, A , Gezercan, Y . "Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?" . Pamukkale Tıp Dergisi 14 (2021 ): 354-360 <https://dergipark.org.tr/tr/pub/patd/issue/60284/789163>
Chicago Olguner, S , Çeliktaş, M , Erdogan, K , Dağlıoğlu, K , İştemen, İ , Arslan, A , Açık, V , Ökten, A , Gezercan, Y . "Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?". Pamukkale Tıp Dergisi 14 (2021 ): 354-360
RIS TY - JOUR T1 - Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis? AU - Semih Kivanc Olguner , Mustafa Çeliktaş , Kivilcim Erdogan , Kenan Dağlıoğlu , İsmail İştemen , Ali Arslan , Vedat Açık , Ali Ökten , Yurdal Gezercan Y1 - 2021 PY - 2021 N1 - doi: 10.31362/patd.789163 DO - 10.31362/patd.789163 T2 - Pamukkale Tıp Dergisi JF - Journal JO - JOR SP - 354 EP - 360 VL - 14 IS - 2 SN - -1308-0865 M3 - doi: 10.31362/patd.789163 UR - https://doi.org/10.31362/patd.789163 Y2 - 2021 ER -
EndNote %0 Pamukkale Tıp Dergisi Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis? %A Semih Kivanc Olguner , Mustafa Çeliktaş , Kivilcim Erdogan , Kenan Dağlıoğlu , İsmail İştemen , Ali Arslan , Vedat Açık , Ali Ökten , Yurdal Gezercan %T Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis? %D 2021 %J Pamukkale Tıp Dergisi %P -1308-0865 %V 14 %N 2 %R doi: 10.31362/patd.789163 %U 10.31362/patd.789163
ISNAD Olguner, Semih Kivanc , Çeliktaş, Mustafa , Erdogan, Kivilcim , Dağlıoğlu, Kenan , İştemen, İsmail , Arslan, Ali , Açık, Vedat , Ökten, Ali , Gezercan, Yurdal . "Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?". Pamukkale Tıp Dergisi 14 / 2 (Nisan 2021): 354-360 . https://doi.org/10.31362/patd.789163
AMA Olguner S , Çeliktaş M , Erdogan K , Dağlıoğlu K , İştemen İ , Arslan A , Açık V , Ökten A , Gezercan Y . Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?. Pam Tıp Derg. 2021; 14(2): 354-360.
Vancouver Olguner S , Çeliktaş M , Erdogan K , Dağlıoğlu K , İştemen İ , Arslan A , Açık V , Ökten A , Gezercan Y . Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?. Pamukkale Tıp Dergisi. 2021; 14(2): 354-360.
IEEE S. Olguner , M. Çeliktaş , K. Erdogan , K. Dağlıoğlu , İ. İştemen , A. Arslan , V. Açık , A. Ökten ve Y. Gezercan , "Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?", Pamukkale Tıp Dergisi, c. 14, sayı. 2, ss. 354-360, Nis. 2021, doi:10.31362/patd.789163