Green Synthesis of Silver Nanoparticles and Their Antimicrobial Effects on Some Food Pathogens

Öz

In this study, silver nanoparticles (AgNPs) were obtained by biological method in a simple, low-cost and environmentally friendly way. The characterization of the synthesized AgNPs was fulfilled using UV-visible Spectrophotometer, Field Emission Scanning Electron Microscope (FE-SEM), Transmission Electron Microscopy (TEM), Energy-Dispersive X-Ray Spectroscopy, X-Ray Diffraction Diffractrometer (XRD), Fourier Transform Infrared Spectroscopy and Zeta sizer and potantial devices. It was determined that AgNPs gave a maximum peak at 440-450 nm absorbance. According to the TEM and FE-SEM results, the morphological structures of the nanoparticles were spherical and their average size was 38 nm. According to the XRD pattern, it was observed that the powder crystal structures of the nanoparticles were cubic and 21.94 nm in size. The zeta size of the nanoparticles was 158.2 nm on average and zeta potential of nanoparticles was -23.4 mV. Minimum Inhibition Concentrations (MIC) (mg/mL) of the synthesized AgNPs on Staphylococcus aureus ATCC 29213, Bacillus subtilis ATCC 11774, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Candida albicans were determined as 1.25, 0.625, 2.5, 1.25 and 0.312, respectively. AgNO3 and antibiotic MIC values (mg/mL) of the mentioned microorganisms were determined as 2.65;2, 1.32;1, 0.66;2, 1.32;4, 0.66;2, respectively.

Anahtar Kelimeler

• AgNP’s
• FE-SEM
• TEM
• XRD
• Antimicrobial effect

Gümüş Nanopartiküllerin Yeşil Sentezi ve Bazı Gıda Patojenleri Üzerindeki Antimikrobiyal Etkileri

Öz

Bu çalışmada, gümüş nanopartiküller (AgNP’ler) biyolojik yöntemle kolay, düşük maliyetli ve çevre dostu bir şekilde elde edilmiştir. Sentezi yapılan AgNP’lerin karakterizasyonu UV-visible Spektrofotometre (UV-Vis.), Alan Emisyon Taramalı Elektron Mikroskobu (FE-SEM), Transmisyon Elektron Mikroskobu (TEM), Enerji Dağılımlı X-Işını Spektroskopisi (EDX), X- Işını Kırınımı Difraktrometresi (XRD), Fourier Dönüşümü Kızılötesi Spektroskopisi (FT-IR) ve Zeta boyut ve potansiyel cihazı kullanılarak yapılmıştır. AgNP’lerin 440-450 nm absorbansta maksimum pik vermiştir. TEM ve FE-SEM sonuçlarına göre nanopartiküllerin morfolojik yapılarının küresel ve ortalama 38 nm; XRD sonuçlarına göre nanopartiküllerin toz kristal yapılarının kübik ve 21.94 nm boyutunda; zeta boyutunun ise ortalama 158.2 nm, zeta potansiyelinin -23.4 mV olduğu görülmüştür. Sentezlenen AgNP'lerin Staphylococcus aureus ATCC 29213, Bacillus subtilis ATCC 11774 Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 ve Candida albicans üzerinde Minimum İnhibisyon Konsantrasyonları (MİK) (mg/mL) sırasıyla 1.25, 0.625, 2.50, 1.25 ve 0.312 olarak tespit edilmiştir. Söz konusu mikroorganizmaların AgNO3 ve antibiyotik MİK değerleri (mg/mL) sırasıyla 2.65;2, 1.32;1, 0.66;2, 1.32;4, 0.66;2 olarak tespit edilmiştir.

Anahtar Kelimeler

• AgNP'ler
• FE-SEM
• TEM
• XRD
• Antimikrobiyal etki

Kaynakça

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