Using Molecular Markers to Improve Potato Lines Resistant to Pathogens

Öz

Potato is a very important crop for human nutrition worldwide. Potato disease and pests can cause economic yield losses. In this study, approximately 130.000 potato seeds from different genetic backgrounds were obtained, evaluated, and selected from 2008 to 2016 for developing new cultivars. After several years, superior lines were tested in different locations to select new cultivars. At the end of the potato breeding program, eight superior potato lines (Nos 12-55-07, 12-55-16, 12-68-05, 12-69-39, 13-67-25, 13-66-23, 12-45-24 and 13-66-75) were submitted for registration as commercial cultivars. The use of resistance genes is the most effective method for controlling these diseases and pests, and DNA markers that are tightly linked to resistance genes are available. The aim of this research was to evaluate the use of closely linked molecular markers for combining Gro1 and H1 for cist nematode resistance, Sen1 for wart resistance or with R1 for late blight or with Rx1 for PVX in advanced breeding lines and commercial candidate cultivars. In the research, 61 advanced lines were checked for the presence of five markers. As a result, only two breeding lines (Nos 31-01-03 and 32-02-52) were determined to have positive results for four markers, and nine advanced lines were found to have positive marker results for Sen1, H1, and R1 at the same time. In 6 advanced lines, positive results were acquired from both Sen1, H1, and Rx1, 2 lines Sen1, Gro1, and H1, and only one line No. 12-45-24 Sen1, Gro1, H1, and Rx1 were found to have positive results for markers. Marker-assisted selection for cist nematode, wart, late blight, and PVX will be performed using potato breeding programs.

Anahtar Kelimeler

• Cyst
• Globodera
• marker
• potato
• cultivar

Kaynakça

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