Comparative Evaluation of Capillary Electrophoresis and Flame Photometry for Sodium, Potassium and Calcium Determination in Bone Broth

Öz

This study aimed to optimize and validate a capillary electrophoresis (CE) method for the quantification of sodium, potassium, and calcium in bone broth and to compare with flame photometry (FP). Separation conditions of cations were optimized using response surface methodology considering the influence of temperature (13-27 oC) and voltage (13-27 kV). It has been found that the optimal conditions (18 oC and 20 kV) provided a high theoretical plate number (efficiency) and resolution with acceptable migration times. CE showed higher linearity (R2 >0.998) and lower limits of detection (1.50-3.46 µg/mL) than FP (3.23-10.5 µg/mL). In terms of precision, the FP showed better intra-day repeatability, whereas CE showed better inter-day repeatability, particularly for K+. Recovery values of cations determined in both methods ranged from 93.7% to 109%. When applied to bone broth samples, no significant differences were observed between CE and FP (p >0.05). Na+ was the most abundant mineral, with concentrations of 1037 and 1049 µg/mL determined by FP and CE, respectively, followed by K+ (166 and 158 µg/mL) and Ca2+ (67.3 and 64.2 µg/mL). Overall, the results showed that CE is a reliable, sensitive, and environmentally friendly alternative to FP for the determination of mineral cations in food matrices.

Anahtar Kelimeler

• Minerals
• Detection limit
• Precision
• Response factor
• Response surface methodology

Proje Numarası

1919B012428356

Kemik Suyu İçindeki Sodyum, Potasyum ve Kalsiyum Tayini İçin Kılcal Elektroforez ve Alev Fotometrisinin Karşılaştırmalı Değerlendirilmesi

Öz

Bu çalışma, kemik suyunda sodyum, potasyum ve kalsiyumun kantitasyonu için bir kapiler elektroforez (CE) yöntemini optimize etmeyi ve doğrulamayı ve alev fotometrisi (FP) ile karşılaştırmayı amaçlamıştır. Katyonların ayrılma koşulları, sıcaklığın (13-27 °C) ve voltajın (13-27 kV) etkisini dikkate alarak yanıt yüzey metodolojisi kullanılarak optimize edilmiştir. Optimal koşulların (18 °C ve 20 kV), kabul edilebilir göç süreleriyle yüksek teorik plaka sayısı (verimlilik) ve çözünürlük sağladığı bulunmuştur. CE, FP'ye (3,23-10,5 µg/mL) kıyasla daha yüksek doğrusallık (R2 >0,998) ve daha düşük tespit limitleri (1,50-3,46 µg/mL) göstermiştir. Hassasiyet açısından, FP daha iyi gün içi tekrarlanabilirlik gösterirken, CE özellikle K+ için daha iyi günler arası tekrarlanabilirlik göstermiştir. Her iki yöntemde belirlenen katyonların geri kazanım değerleri %93,7 ile %109 arasında değişmiştir. Kemik suyu örneklerine uygulandığında, CE ve FP arasında anlamlı bir fark gözlenmedi (p > 0,05). En bol bulunan mineral Na+ olup, FP ve CE tarafından sırasıyla 1037 ve 1049 µg/mL konsantrasyonları belirlenmiştir; bunu K+ (166 ve 158 µg/mL) ve Ca2+ (67,3 ve 64,2 µg/mL) izlemiştir. Genel olarak, sonuçlar CE'nin gıda matrislerindeki mineral katyonlarının belirlenmesi için FP'ye güvenilir, hassas ve çevre dostu bir alternatif olduğunu göstermiştir.

Anahtar Kelimeler

• Mineraller
• Tespit limiti
• Hassasiyet
• Tepki faktörü
• Tepki yüzeyi metodolojisi

Proje Numarası

1919B012428356

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