Comparative evaluation of bioactive properties of Philadelphus coronarius leaf and flower extracts: Antioxidant and enzyme inhibitory activities

Öz

This study comparatively evaluated the bioactive properties of Philadelphus coronarius leaf and flower extracts, focusing on phenolic composition, antioxidant capacity, and enzyme inhibitory activities. Plant materials were collected, dried, powdered, and extracted using 80% ethanol via ultrasound-assisted extraction. Total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities (ABTS, DPPH, and FRAP assays) were determined, along with α-glucosidase and acetylcholinesterase inhibitory activities. The leaf extract exhibited significantly higher phenolic and flavonoid contents (4082.81 mg GAE/100 g DW and 3444.17 mg/100 g DW, respectively) compared to the flower extract (1864.06 mg GAE/100 g DW and 869.17 mg/100 g DW). Similarly, the antioxidant capacities (DPPH, ABTS, and FRAP values) of the leaf extract were found to be significantly higher than those of the flower extract. In enzyme inhibition studies, the leaf extract demonstrated stronger α-glucosidase inhibitory activity (IC50: 0.0211 mg/mL) than the flower extract (IC50: 0.0523 mg/mL). In contrast, acetylcholinesterase inhibition was similar for both extracts, Overall, the findings indicate that P. coronarius leaves possess a strong bioactive potential, particularly in terms of phenolic compounds, antioxidant capacity, and α-glucosidase inhibitory activity. Therefore, the leaf part of this species may be considered a promising natural source of antioxidants and functional bioactive compounds.

Anahtar Kelimeler

• Philadelphus coronarius
• phenolic
• antioxidant activity
• α-glucosidase inhibition
• acetylcholinesterase inhibition

Philadelphus coronarius yaprağı ve çiçeği özlerinin biyoaktif özelliklerinin karşılaştırmalı değerlendirmesi: antioksidan ve enzim inhibitör aktiviteleri

Öz

Bu çalışma, Philadelphus coronarius yaprak ve çiçek özütlerinin biyoaktif özelliklerini, fenolik bileşim, antioksidan kapasite ve enzim inhibitör aktiviteleri açısından karşılaştırmalı olarak değerlendirmiştir. Bitki materyalleri toplanmış, kurutulmuş, toz haline getirilmiş ve ultrason destekli ekstraksiyon yöntemiyle %80 etanol kullanılarak özütlenmiştir. Toplam fenolik içerik (TPC), toplam flavonoid içerik (TFC) ve antioksidan aktiviteler (ABTS, DPPH ve FRAP testleri) ile birlikte α-glukozidaz ve asetilkolinesteraz inhibitör aktiviteleri belirlenmiştir. Yaprak özütü, çiçek özütüne kıyasla önemli ölçüde daha yüksek fenolik ve flavonoid içerik (sırasıyla 4082,81 mg GAE/100 g DW ve 3444,17 mg/100 g DW) göstermiştir (çiçek özütü için bu değerler sırasıyla 1864,06 mg GAE/100 g DW ve 869,17 mg/100 g DW'dir). Benzer şekilde, yaprak özütünün antioksidan kapasitelerinin (DPPH, ABTS ve FRAP değerleri) çiçek özütüne göre önemli ölçüde daha yüksek olduğu bulunmuştur. Enzim inhibisyon çalışmalarında, yaprak özütü (IC50: 0,0211 mg/mL), çiçek özütüne (IC50: 0,0523 mg/mL) kıyasla daha güçlü α-glukozidaz inhibitör aktivitesi göstermiştir. Buna karşılık, asetilkolinesteraz inhibisyonu her iki özüt için de benzerdi. Genel olarak, bulgular P. coronarius yapraklarının, özellikle fenolik bileşikler, antioksidan kapasite ve α-glukozidaz inhibitör aktivitesi açısından güçlü bir biyoaktif potansiyele sahip olduğunu göstermektedir. Bu nedenle, bu türün yaprak kısmı, antioksidanlar ve fonksiyonel biyoaktif bileşikler için umut vadeden doğal bir kaynak olarak değerlendirilebilir.

Anahtar Kelimeler

• Philadelphus coronarius
• fenol
• antioksidan aktivite
• α-glukozidaz inhibisyonu
• asetilkolinesteraz inhibisyonu

Kaynakça

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