Araştırma Makalesi
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Studying Structural Differentiation of Plant Parts of Sideritis pisidica Boiss. & Heldr. Using FTIR Spectroscopy

Yıl 2017, Cilt: 4 Sayı: 4, 461 - 467, 26.10.2017

Öz

Fourier
Transform Infrared Spectroscopy (FTIR) is a novel tool for exploring
compositional structures of diverse organic or inorganic compounds employed by
many researches from physicists to horticulturists due to its time-saving and
easy-to-use application. FTIR was performed to differentiate structural
differentiation of plant parts of wild-grown Sideritis pisidica Boiss. & Heldr. from Turkey. Some minerals
Na, Cr, Zn, Fe, Mg, Mn, Co, Cu and Ni; protein, lipid, soluble sugar and
moisture contents in the stalk, leaf and blossom of the plant were analyzed
whether the FTIR spectra may predict major structural compositions. As expected
most of the minerals were presented higher amount in the leaf followed by the
blossom and stalk. The blossom was however found be richer in Ca, Cr, Ni and Zn
than leaf and stalk. Total protein contents of the leaf were higher than those
of the stalk or the leaf. Crude lipid amounts were however more prominent in
the stalk followed by the blossom and leaf respectively, unlike the total
protein contents. The blossom was found be richer in moisture content than the
leaf and stalk followed, respectively. The FTIR spectra of the plant parts
clearly revealed that protein and total soluble sugar contents are varied in
stalk, leaf or blossom of Sideritis
pisidica
Boiss. & Heldr. interpreted from the peak intensities. The
spectra however did not reveal the crude lipid or moisture contents of the
plant parts.

Kaynakça

  • Ağaçkayak, T., Zedef, V., Aydoğan, S. 2006. Investigation of enrichment possibilities of chromite Ore of Yesşildağ (Beysehir - Konya) by flotation. Selçuk University, Journal of Engineering and Architectural Faculty, 21(1-2): 153-159.
  • Amir, R.M., Anjum, F.M., Khan, M.I., Khan, M.R., Pasha, I., Nadeem, M. 2013 Application of fourier transform infrared (FTIR) spectroscopy for the identification of wheat varieties. Journal of Food Science and Technology, 50(5): 1018-1023.
  • Basnet, P., Amarasiriwardena, D., Wu, F., Fu, Z., Zhang, T. 2016. Investigation of tissue level distribution of functional groups and associated trace metals in rice seeds (Oryza sativa L.) using FTIR and LA-ICP-MS. Microchemical Journal, 127: 152-159.
  • Bellamy, L.J. 1975. Infrared Spectra of Complex Molecules. Chapman Hall, London, UK.
  • Cai, S., Singh, R.B. 2004. A distinct utility of the amide III infrared band for secondary structure estimation of aqueous protein solutions using partial least squares methods. Biochemistry, 43: 2541-2549.
  • Dick, D.P., Knicker, H., Ávila, L.G., Inda Jr., A.V., Giasson, E., Bissani, C.A. 2006. Organic matter in constructed soils from a coal mining area in southern Brazil. Organic Geochemistry, 37: 1537-1545.
  • Ergun, M., Ergun, N., Ozbay, N. 2016. Analysis of volatile constituents of Sideritis pisidica Boiss. & Heldr. by head space gas chromatography mass spectrometry (HS-GC/MS). Zeitschrift für Arznei & Gewürzpflanzen, 21(2): 68-72.
  • Gallardo-Velazquez, T., Osorio-Revilla, G., Loa, M.Z., Rivera-Espinoza, Y. 2009. Application of FTIR–HATR spectroscopy and multivariate analysis to the quantification of adulterants in Mexican honeys. Food Research International., 42 (3): 313-318.
  • Geethu, M.G., Suchithra, P.S., Kavitha, C.H., Aswathy, J.M., Dinesh, B., Murugan, K. 2014. Fourier-transform infrared spectroscopy analysis of different solvent extracts of water hyacinth (Eichhornia crassipes mart solms.) an allelopathic approach. World Journal of Pharmacy and Pharmaceutical Sciences, 3(6): 1256-1266.
  • Güvenç, M., Duman, H. 2010. Morphological and anatomical studies of annual taxa of Sideritis L. (Lamiaceae), with notes on chorology in Turkey. Turkish Journal of Botany, 34: 83-104.
  • Ibrahim, M., Nada, A., Lamal, D.E. 2005. Density functional theory and FTIR spectroscopic study of carboxyl group. Indian Journal of Pure & Applied Physics, 43: 911-917.
  • Jentzsch, P.V., Ramos, L., Ciobota, V. 2015. Handheld Raman spectroscopy for the distinction of essential oils used in the cosmetics industry. Cosmetics, 2: 162-176.
  • Kaposi, A.D., Fidy, J., Manas, E.S., Vanderkooi, J.M., Wright, W.W. 1999. Horseradish peroxidase monitored by infrared spectroscopy: e¡ect of temperature, substrate and calcium. Biochimica et Biophysica Acta, 1435: 41-50.
  • Karapandzova, M., Qazimi, B., Stefkov, G., Bačeva, K., Stafilov, T., Panovska, T.K., Kulevanova, S. 2013. Chemical Characterization, Mineral Content and Radical Scavenging Activity of Sideritis scardica and S. raeseri from R. Macedonia and R. Albania. Natural Product Communications, 8(5), 693-644.
  • Koc, H., Sari, H. 2009. Trace metal contents of some medicinal, aromatic plants and soil samples in the Mediterranean Region, Turkey. Journal of Applied Chemical Research, 8: 52-57.
  • Li, Y.M., Sun, S.Q., Zhou, Q., Qin, Z., Tao, J.X., Wang, J., Fang. X. 2004. Identification of American ginseng from different regions using FT-IR and two-dimensional correlation IR spectroscopy. Vibrational spectroscopy, 36: 227-232.
  • Mathlouthi, M.,, Koenig, L.L. 1986. Vibrational spectra of carbohydrates. Advances in Carbohydrate Chemistry and Biochemistry, 44: 7-89.
  • Mazurek, S., Mucciolo, A., Humbel, B.M., Nawrath, C. 2013. Transmission Fourier transform infrared microspectroscopy allows simultaneous assessment of cutin and cell-wall polysaccharides of Arabidopsis petals. The Plant Journal, 74: 880-891.
  • Movasaghi, Z., Rehman, S., ur Rehman, I. 2008. Fourier transform infrared (FTIR) spectroscopy of biological tissues. Applied Spectroscopy Reviews, 43: 134-179.
  • Parikh, S.J., Chorover, J. 2007. Infrared spectroscopy studies of cation effects on lipopolysaccharides in aqueous solution. Colloids and Surfaces B: Biointerfaces, 55: 41-250.
  • Pljevljakusic, D., Savikin, K., Jankovic, T., Zdunic, G., Ristic, M., Godjevac, D., Konic-Ristic, A. 2011. Chemical properties of the cultivated Sideritis raeseri Boiss. & Heldr. subsp. raeseri. Food Chemistry, 124: 226-233.
  • Philip, D. 2009. Honey mediated green synthesis of gold nanoparticles. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 73(4): 650-653.
  • Pradhan, J., Das, S.N., Thaker, R.S. 2007. Adsorption of hexavalent chromium from aqueous solution by using activated red mud. Journal of Colloid Interface Science, 217(1): 137-141.
  • Prasad, A.G., Kumar, J.K., Sharanappa, P. 2011. Fourier transform infrared spectroscopic study of rare and endangered medicinal plants. Romanian Journal of Biophysics, 21(3): 221-230.
  • Sivakesava, S., Irudayaraj, J. 2001. Prediction of inverted cane sugar adulteration of honey by Fourier transform infrared spectroscopy. Journal of Food Science, 66: 972-978.
  • Stuart, B. 1997. Biological applications of infrared spectroscopy. Chichester, UK: ACOL Series, Wiley.
  • Subari, N., Saleh, J.M., Shakaff, A.Y.M., Zakaria, A. 2012. A hybrid sensing approach for pure and adulterated honey classification. Sensors, 12: 14022-14040.
  • Tewari, J., Irudayaraj, J. 2004. Quantification of saccharides in multiple floral honeys using Fourier transform infrared micro attenuated total reflectance spectroscopy. Journal of Agricultural Food Chemistry, 52, 3237-3243.
  • Tewari, J.C., Irudayaraj, J.M.K. 2005. Floral classification of honey using mid-infrared spectroscopy and surface acoustic wave based z-Nose sensor. Journal of Agricultural Food Chemistry, 53: 6955-6966.
  • Yücel, E., Tapırdamaz, A., Yücel Şengün, İ., Yılmaz, G., Ak, A. 2011. Kisecik Kasabası (Karaman) ve çevresinde bulunan bazı yabani bitkilerin kullanım biçimleri ve besin öğesi içeriklerinin belirlenmesi. Biological Diversity and Conversation, (4/3): 71-82.
  • Varetti, E.L., Volponi, C.R. 1995. Characterization of crystals in plant cells using FT-IR microspectroscopy. Applied Spectroscopy, 49(4): 537. 539.

FTIR Spektroskopisi Kullanarak Sideritis pisidica Boiss. & Heldr. Bitki Aksamlarındaki Yapısal Farklılığın Araştırılması

Yıl 2017, Cilt: 4 Sayı: 4, 461 - 467, 26.10.2017

Öz

Fourier dönüşümlü
kızılötesi spektroskopisi (FTIR) kısa sürede ve kolay uygulanabilmesi nedeni
ile fizik bilim insanlarından bahçe bitkileri bilim insanlarına kadar birçok
araştırmacının başvurduğu değişik organik ve inorganik maddelerin yapılarını
araştırmak amacıyla kullanılan yenilikçi bir yöntemdir. Bu çalışmada, FTIR,
Türkiye’de doğal olarak yetişen yabani Sideritis
pisidica
Boiss. & Heldr. bitki aksamlarındaki farklılığı ortaya koymak
için kullanılmıştır. Bitkinin gövdesi, yaprakları ve çiçeklerinde bulunan Na,
Cr, Zn, Fe, Mg, Mn, Co, Cu ve Ni gibi bazı mineraller ile protein, yağ, çözünür
şeker ve nem içeriği, FTIR spektrumlarının ana yapısal içerikleri tahmin edip
edemeyeceği amacıyla analiz edilmiştir. Tahmin edildiği gibi, çoğu minerallerin
miktarı yaprakta yüksek miktarda bulunmuş ve yaprağı sırası ile çiçek ve gövde
takip etmiştir. Bununla birlikte çiçek, yaprak ve gövdeye göre Ca, Cr ve Zn
mineralleri bakımından daha zengin bir içeriğe sahip olmuştur. Yaprak, gövde ve
çiçeğe göre daha fazla protein içermiştir. Toplam proteinin aksine, ham yağ
içeriği gövdede en yüksek bulunmuş ve gövdeyi sırası ile çiçek ve yaprak takip
etmiştir. Pik içeriği dikkate alındığında, FTIR spektrumları Sideritis pisidica Boiss. & Heldr.
bitkisinin gövde, yaprak ve çiçekte bulunan protein veya toplam çözünür şeker
içeriklerindeki farklığı açıkça ortaya koymuştur. Fakat spektrumlar bitki
aksamlarındaki ham yağ veya nem içeriğini tahmin edememiştir.



 

Kaynakça

  • Ağaçkayak, T., Zedef, V., Aydoğan, S. 2006. Investigation of enrichment possibilities of chromite Ore of Yesşildağ (Beysehir - Konya) by flotation. Selçuk University, Journal of Engineering and Architectural Faculty, 21(1-2): 153-159.
  • Amir, R.M., Anjum, F.M., Khan, M.I., Khan, M.R., Pasha, I., Nadeem, M. 2013 Application of fourier transform infrared (FTIR) spectroscopy for the identification of wheat varieties. Journal of Food Science and Technology, 50(5): 1018-1023.
  • Basnet, P., Amarasiriwardena, D., Wu, F., Fu, Z., Zhang, T. 2016. Investigation of tissue level distribution of functional groups and associated trace metals in rice seeds (Oryza sativa L.) using FTIR and LA-ICP-MS. Microchemical Journal, 127: 152-159.
  • Bellamy, L.J. 1975. Infrared Spectra of Complex Molecules. Chapman Hall, London, UK.
  • Cai, S., Singh, R.B. 2004. A distinct utility of the amide III infrared band for secondary structure estimation of aqueous protein solutions using partial least squares methods. Biochemistry, 43: 2541-2549.
  • Dick, D.P., Knicker, H., Ávila, L.G., Inda Jr., A.V., Giasson, E., Bissani, C.A. 2006. Organic matter in constructed soils from a coal mining area in southern Brazil. Organic Geochemistry, 37: 1537-1545.
  • Ergun, M., Ergun, N., Ozbay, N. 2016. Analysis of volatile constituents of Sideritis pisidica Boiss. & Heldr. by head space gas chromatography mass spectrometry (HS-GC/MS). Zeitschrift für Arznei & Gewürzpflanzen, 21(2): 68-72.
  • Gallardo-Velazquez, T., Osorio-Revilla, G., Loa, M.Z., Rivera-Espinoza, Y. 2009. Application of FTIR–HATR spectroscopy and multivariate analysis to the quantification of adulterants in Mexican honeys. Food Research International., 42 (3): 313-318.
  • Geethu, M.G., Suchithra, P.S., Kavitha, C.H., Aswathy, J.M., Dinesh, B., Murugan, K. 2014. Fourier-transform infrared spectroscopy analysis of different solvent extracts of water hyacinth (Eichhornia crassipes mart solms.) an allelopathic approach. World Journal of Pharmacy and Pharmaceutical Sciences, 3(6): 1256-1266.
  • Güvenç, M., Duman, H. 2010. Morphological and anatomical studies of annual taxa of Sideritis L. (Lamiaceae), with notes on chorology in Turkey. Turkish Journal of Botany, 34: 83-104.
  • Ibrahim, M., Nada, A., Lamal, D.E. 2005. Density functional theory and FTIR spectroscopic study of carboxyl group. Indian Journal of Pure & Applied Physics, 43: 911-917.
  • Jentzsch, P.V., Ramos, L., Ciobota, V. 2015. Handheld Raman spectroscopy for the distinction of essential oils used in the cosmetics industry. Cosmetics, 2: 162-176.
  • Kaposi, A.D., Fidy, J., Manas, E.S., Vanderkooi, J.M., Wright, W.W. 1999. Horseradish peroxidase monitored by infrared spectroscopy: e¡ect of temperature, substrate and calcium. Biochimica et Biophysica Acta, 1435: 41-50.
  • Karapandzova, M., Qazimi, B., Stefkov, G., Bačeva, K., Stafilov, T., Panovska, T.K., Kulevanova, S. 2013. Chemical Characterization, Mineral Content and Radical Scavenging Activity of Sideritis scardica and S. raeseri from R. Macedonia and R. Albania. Natural Product Communications, 8(5), 693-644.
  • Koc, H., Sari, H. 2009. Trace metal contents of some medicinal, aromatic plants and soil samples in the Mediterranean Region, Turkey. Journal of Applied Chemical Research, 8: 52-57.
  • Li, Y.M., Sun, S.Q., Zhou, Q., Qin, Z., Tao, J.X., Wang, J., Fang. X. 2004. Identification of American ginseng from different regions using FT-IR and two-dimensional correlation IR spectroscopy. Vibrational spectroscopy, 36: 227-232.
  • Mathlouthi, M.,, Koenig, L.L. 1986. Vibrational spectra of carbohydrates. Advances in Carbohydrate Chemistry and Biochemistry, 44: 7-89.
  • Mazurek, S., Mucciolo, A., Humbel, B.M., Nawrath, C. 2013. Transmission Fourier transform infrared microspectroscopy allows simultaneous assessment of cutin and cell-wall polysaccharides of Arabidopsis petals. The Plant Journal, 74: 880-891.
  • Movasaghi, Z., Rehman, S., ur Rehman, I. 2008. Fourier transform infrared (FTIR) spectroscopy of biological tissues. Applied Spectroscopy Reviews, 43: 134-179.
  • Parikh, S.J., Chorover, J. 2007. Infrared spectroscopy studies of cation effects on lipopolysaccharides in aqueous solution. Colloids and Surfaces B: Biointerfaces, 55: 41-250.
  • Pljevljakusic, D., Savikin, K., Jankovic, T., Zdunic, G., Ristic, M., Godjevac, D., Konic-Ristic, A. 2011. Chemical properties of the cultivated Sideritis raeseri Boiss. & Heldr. subsp. raeseri. Food Chemistry, 124: 226-233.
  • Philip, D. 2009. Honey mediated green synthesis of gold nanoparticles. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 73(4): 650-653.
  • Pradhan, J., Das, S.N., Thaker, R.S. 2007. Adsorption of hexavalent chromium from aqueous solution by using activated red mud. Journal of Colloid Interface Science, 217(1): 137-141.
  • Prasad, A.G., Kumar, J.K., Sharanappa, P. 2011. Fourier transform infrared spectroscopic study of rare and endangered medicinal plants. Romanian Journal of Biophysics, 21(3): 221-230.
  • Sivakesava, S., Irudayaraj, J. 2001. Prediction of inverted cane sugar adulteration of honey by Fourier transform infrared spectroscopy. Journal of Food Science, 66: 972-978.
  • Stuart, B. 1997. Biological applications of infrared spectroscopy. Chichester, UK: ACOL Series, Wiley.
  • Subari, N., Saleh, J.M., Shakaff, A.Y.M., Zakaria, A. 2012. A hybrid sensing approach for pure and adulterated honey classification. Sensors, 12: 14022-14040.
  • Tewari, J., Irudayaraj, J. 2004. Quantification of saccharides in multiple floral honeys using Fourier transform infrared micro attenuated total reflectance spectroscopy. Journal of Agricultural Food Chemistry, 52, 3237-3243.
  • Tewari, J.C., Irudayaraj, J.M.K. 2005. Floral classification of honey using mid-infrared spectroscopy and surface acoustic wave based z-Nose sensor. Journal of Agricultural Food Chemistry, 53: 6955-6966.
  • Yücel, E., Tapırdamaz, A., Yücel Şengün, İ., Yılmaz, G., Ak, A. 2011. Kisecik Kasabası (Karaman) ve çevresinde bulunan bazı yabani bitkilerin kullanım biçimleri ve besin öğesi içeriklerinin belirlenmesi. Biological Diversity and Conversation, (4/3): 71-82.
  • Varetti, E.L., Volponi, C.R. 1995. Characterization of crystals in plant cells using FT-IR microspectroscopy. Applied Spectroscopy, 49(4): 537. 539.
Toplam 31 adet kaynakça vardır.

Ayrıntılar

Bölüm Araştırma Makaleleri
Yazarlar

Muharrem Ergun

Zahide Süslüoğlu Bu kişi benim

Aydın Şükrü Bengü Bu kişi benim

Yayımlanma Tarihi 26 Ekim 2017
Gönderilme Tarihi 26 Ekim 2017
Yayımlandığı Sayı Yıl 2017 Cilt: 4 Sayı: 4

Kaynak Göster

APA Ergun, M., Süslüoğlu, Z., & Bengü, A. Ş. (2017). FTIR Spektroskopisi Kullanarak Sideritis pisidica Boiss. & Heldr. Bitki Aksamlarındaki Yapısal Farklılığın Araştırılması. Türk Tarım Ve Doğa Bilimleri Dergisi, 4(4), 461-467.