PROPOLİS VE ARI SÜTÜ İÇEREN CİLT KREMİ: ANTİOKSİDAN, ANTİ-HYALÜRONİDAZ VE ANTİMİKROBİYAL AKTİVİTELERİN DEĞERLENDİRMESİ

Yıl 2023, Cilt: 23 Sayı: 2, 224 - 238, 19.11.2023

Gülsüm Merve Boyracı , Atiye Değirmenci , Oktay Yıldız , Zeynep Berin Çelebi

https://doi.org/10.31467/uluaricilik.1355264

Öz

Propolis ve arı sütü eski çağlardan beri geleneksel ve modern tıpta çeşitli biyolojik aktivitelerinden dolayı kullanılmıştır. Günümüzde yapılan son çalışmalar bu ürünlerin cilt tedavisi ve bakımı alanındaki potansiyel uygulamalarını ortaya koymuştur. Bu çalışmanın amacı, cilt kremi üretiminde propolis ve arı sütünün potansiyel kullanımını incelemek ve arı ürünleriyle zenginleştirilmiş cilt kremi formülasyonunun anti-hiyalüronidaz, antioksidan ve antimikrobiyal aktivitesini değerlendirmektir. Ayrıca kremlerin farklı depolama sıcaklığı ve süresindeki fiziksel stabilitesi de incelenmiştir. Krem formülasyonunda arı sütü oranı (%0,5) sabit tutulurken propolis özütü %0,5-1,0-1,5 oranlarında kullanılmıştır. Çalışmada üretilen kremlerin pH değerleri 5,56 ile 6,02 aralığında değişmekte olup cilt pH değerine uygun bulunmuştur. Krem formülasyonunda propolis oranı arttıkça antioksidan aktivite değerlerinin istatistiksel olarak arttığı görülmüştür (p≤0,05). Krem örneklerinin anti-hiyalüronidaz aktivite analizi sonucu IC50 değerleri 0,1667-3,3460 g/mL krem olarak belirlenmiş ve en yüksek aktivite %1,5 propolis özütü ilaveli örnekte bulunmuştur. Propolis ve arı sütü ilaveli krem örneklerinin tamamı Bacillus cereus, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis ve Candida albicans mikroorganizmalarına karşı inhibisyon göstermiştir. Hızlandırılmış stabilite testleri krem formülasyonun tüm stres koşullarına karşı dikkate değer bir stabiliteye sahip olduğunu göstermiştir. Bu çalışma kozmetik sektöründe doğal, sürdürülebilir ve güvenli cilt bakım ürünleri üretebilmek için krem formülasyonuna arı ürünlerini entegre etmenin uygulanabilirliğine dair kanıt sağlamaktadır.

Anahtar Kelimeler

Propolis, Arı sütü, Krem, Cilt yenileyici etki, Anti-hiyaluronidaz aktivite

Etik Beyan

Bu araştırma için etik kurul belgesi gerekli değildir.

Destekleyen Kurum

TÜBİTAK 2209-B Sanayiye Yönelik Lisans Araştırma Projeleri Destekleme Programına

Teşekkür

Desteklerinden ötürü TÜBİTAK 2209-B Sanayiye Yönelik Lisans Araştırma Projeleri Destekleme Programına teşekkür ederiz.

Propolis and royal jelly containing skin cream: The evaluation of antioxidant, anti-hyaluronidase, and antimicrobial activities

Öz

Propolis and royal jelly have been utilized in both traditional and modern medical practices since earlier times, owing to their diverse range of biological properties. Recent research has demonstrated their potential application in the realm of skin treatment and care. The purpose of this study is to examine the potential application of propolis and royal jelly in cream formulation and to evaluate the impact of the utilized bee products on the cream's anti-hyaluronidase, antioxidant, and antimicrobial activities. It is also investigate the physical stability of creams at various storage temperatures and durations. The cream formulation maintained ratio of royal jelly at 0.5%, while varying amounts of propolis extract ranging from 0.5% to 1.0% to 1.5% were utilized. The pH values of the creams examined in the study ranged from 5.56 to 6.02, indicating that they were compatible with the pH value of the skin. It was observed that the antioxidant activity values increased statistically as the propolis ratio increased in the cream formulation. As a result of the antihyaluronidase activity analysis of the cream samples, the IC50 values were determined as 0.16672-3.34603 g/ml cream and the Cream 3 sample with 1.5% propolis extract showed the highest activity. The cream samples with propolis and royal jelly exhibited inhibitory activity against Bacillus cereus, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Candida albicans. The results of accelerated stability testing indicate that the cream formulation exhibits exceptional stability when subjected to various stress situations. This research provides evidence of the feasibility of integrating bee products into cream formulations to enrich them with natural, sustainable and safe skin care products in the cosmetics industry.

Anahtar Kelimeler

Propolis, Royal jelly, Cream, Skin regenerating effect, Anti-hyaluronidase activity

Kaynakça

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