Araştırma Makalesi
PDF Zotero Mendeley EndNote BibTex Kaynak Göster

Erianinin HT29 Kolorektal Kanser Hücrelerinin Proliferasyonu ve Koloni Oluşumu Üzerine Etkilerinin Araştırılması

Yıl 2021, Cilt 47, Sayı 2, 219 - 223, 01.08.2021
https://doi.org/10.32708/uutfd.917751

Öz

Kolorektal kanser, dünyada kanser ilişkili ölümlerin en yaygın dördüncü sebebidir. Erianin antioksidan ve anti-tümör etkilere sahip Dendrobium ekstraktından elde edilen yeni bir dibenzil bileşiğidir. Bu çalışmada, erianinin HT29 kolorektal kanser hücreleri üzerine olan terapötik etkileri araştırılmıştır. Erianinin HT29 hücre canlılığı üzerine etkileri XTT test ile koloni oluşumu üzerine etkileri ise koloni formasyonu ile değerlendirilmiştir. Erianinin HT29 hücrelerinde IC50 değeri 48. saatte 59.05 μM olarak belirlenmiştir. HT29 hücre dizisinde erianin uygulanan grupta koloni sayısı 67±33 iken kontrol grubunda 350±89 olarak hesaplanmıştır. Erianin, HT29 kolorektal kanser hücrelerinde koloni oluşumunu ise anlamlı derecede azaltmıştır. Yapılan çalışmaların sonuçları, erianinin kolorektal kanser tedavisinde doğal elde edilen bir bileşik olarak güvenli, kolay ulaşılabilir ve umut veren terapötik bir ilaç olabileceğini destekler niteliktedir. Gelecekte erianinin kolorektal kanser hücreleri üzerindeki etki mekanizmasını aydınlatacak daha kapsamlı ve çok merkezli desteklenecek ileri düzeyde klinik çalışmalara ihtiyaç vardır.

Kaynakça

  • An BC, Hong S, Park HJ, vd. Anti-Colorectal Cancer Effects of Probiotic-Derived p8 Protein. Genes 2019; 10(8). doi:10.3390/genes10080624
  • Weng W, Goel A. Curcumin and colorectal cancer: An update and current perspective on this natural medicine. Seminars in Cancer Biology 2020. doi:10.1016/j.semcancer.2020.02.011
  • Huang X mei, Yang Z jie, Xie Q, Zhang Z kang, Zhang H, Ma J ying. Natural products for treating colorectal cancer: A mechanistic review. Biomedicine and Pharmacotherapy 2019; 117: 109142. doi:10.1016/j.biopha.2019.109142
  • Chung SS, Dutta P, Chard N, vd. A novel curcumin analog inhibits canonical and non-canonical functions of telomerase through STAT3 and NF-κB inactivation in colorectal cancer cells. Oncotarget 2019; 10(44): 4516–4531. doi:10.18632/oncotarget.27000
  • Gamage CDB, Park SY, Yang Y, vd. Deoxypodophyllotoxin exerts anti-cancer effects on colorectal cancer cells through induction of apoptosis and suppression of tumorigenesis. International Journal of Molecular Sciences 2019; 20(11): 2612. doi:10.3390/ijms20112612
  • Wang S, Wang L, Zhou Z, vd. Leucovorin Enhances the Anti-cancer Effect of Bortezomib in Colorectal Cancer Cells. Scientific Reports 2017; 7(1): 682 doi:10.1038/s41598-017-00839-9
  • Wang X, Li T. Ropivacaine inhibits the proliferation and migration of colorectal cancer cells through ITGB1. Bioengineered 2021; 12(1): 44–53. doi:10.1080/21655979.2020.1857120
  • Hlosrichok A, Sumkhemthong S, Sritularak B, Chanvorachote P, Chaotham C. A bibenzyl from Dendrobium ellipsophyllum induces apoptosis in human lung cancer cells. Journal of Natural Medicines 2018; 72(3): 615–625. doi:10.1007/s11418-018-1186-x
  • Guo Z, Zhou Y, Yang J, Shao X. Dendrobium candidum extract inhibits proliferation and induces apoptosis of liver cancer cells by inactivating Wnt/β-catenin signaling pathway. Biomedicine and Pharmacotherapy 2019; 110: 371–379. doi:10.1016/j.biopha.2018.11.149
  • Yang L, Hu Y, Zhou G, Chen Q, Song Z. Erianin suppresses hepatocellular carcinoma cells through down-regulation of PI3K/AKT, p38 and ERK MAPK signaling pathways. Bioscience reports 2020; 40(7): BSR20193137. doi:10.1042/BSR20193137
  • Li M, He Y, Peng C, Xie X, Hu G. Erianin inhibits human cervical cancer cell through regulation of tumor protein p53 via the extracellular signal-regulated kinase signaling pathway. Oncology Letters 2018; 16(4): 5006–5012. doi:10.3892/ol.2018.9267
  • Zhu Q, Sheng Y, Li W, vd. Erianin, a novel dibenzyl compound in Dendrobium extract, inhibits bladder cancer cell growth via the mitochondrial apoptosis and JNK pathways. Toxicology and Applied Pharmacology 2019; 371: 41–54. doi:10.1016/j.taap.2019.03.027
  • Wang H, Zhang T, Sun W, vd. Erianin induces G2/M-phase arrest, apoptosis, and autophagy via the ROS/JNK signaling pathway in human osteosarcoma cells in vitro and in vivo. Cell Death and Disease 2016; 7(6): e2247. doi:10.1038/cddis.2016.138
  • Chen P, Wu Q, Feng J, vd. Erianin, a novel dibenzyl compound in Dendrobium extract, inhibits lung cancer cell growth and migration via calcium/calmodulin-dependent ferroptosis. Signal Transduction and Targeted Therapy. 2020; 5(1): 51. doi:10.1038/s41392-020-0149-3
  • Sun J, Fu X, Wang Y, vd. Erianin inhibits the proliferation of T47D cells by inhibiting cell cycles, inducing apoptosis and suppressing migration. American journal of translational research. 2016;8(7):3077–3086.
  • Rajendran V, Jain MV. In vitro tumorigenic assay: colony forming assay for cancer stem cells. Içinde: Methods in Molecular Biology 2018; 1692: 89–95. doi:10.1007/978-1-4939-7401-6_8
  • Franken NAP, Rodermond HM, Stap J, Haveman J, van Bree C. Clonogenic assay of cells in vitro. Nature Protocols 2006; 1(5): 2315–2319. doi:10.1038/nprot.2006.339
  • Brenner H, Kloor M, Pox CP. Colorectal cancer. The Lancet 2014; 383: 1490–1502. doi:10.1016/S0140-6736(13)61649-9
  • Ruiz de Porras V, Layos L, Martínez-Balibrea E. Curcumin: A therapeutic strategy for colorectal cancer? Seminars in Cancer Biology 2021; 73: 321–330. doi:10.1016/j.semcancer.2020.09.004
  • Khan I, Mahfooz S, Ansari IA. Antiproliferative and Apoptotic Properties of Andrographolide Against Human Colon Cancer DLD1 Cell Line. Endocrine, Metabolic & Immune Disorders - Drug Targets 2019; 20(6): 930–942. doi:10.2174/1871530319666191125111920
  • Liu YT, Hsieh MJ, Lin JT, vd. Erianin induces cell apoptosis through ERK pathway in human nasopharyngeal carcinoma. Biomedicine and Pharmacotherapy 2019; 111: 262–269. doi:10.1016/j.biopha.2018.12.081
  • Zhu Q, Sheng Y, Li W, vd. Erianin, a novel dibenzyl compound in Dendrobium extract, inhibits bladder cancer cell growth via the mitochondrial apoptosis and JNK pathways. Toxicology and Applied Pharmacology 2019; 371: 41–54. doi:10.1016/j.taap.2019.03.027
  • Cho JG, Lim KH, Park SG. MED28 increases the colony-forming ability of breast cancer cells by stabilizing the ZNF224 protein upon DNA damage. Oncology Letters 2018; 15(3): 3147–3154. doi:10.3892/ol.2017.7718

Investigation of the effects of erianin on proliferation and colony formation of HT29 colorectal cancer cells

Yıl 2021, Cilt 47, Sayı 2, 219 - 223, 01.08.2021
https://doi.org/10.32708/uutfd.917751

Öz

Colorectal cancer (CRC) is the 4th most common cause of cancer-related death in the global world. Erianin, a novel dibenzyl compound in Dendrobium extract, has antioxidative and antitumor activities. In this study, the therapeutic effects of erianin on HT29 colorectal cancer cells were investigated. Effects of erianin on cell viability were evaluated by XTT test. Effects of erianin on colony formation were evaluated by colony formation analysis. IC50 values of Erianin on HT29 cells were determined as 59.05 μM at 48th hour. While the number of colonies in the HT29 cell line was 67±33 in the erianin treated group, it was calculated as 350±89 in the control group. Erianin significantly reduced colony formation in HT29 colorectal cancer cells. The results of the presented studies support that erianin as a natural product in the treatment of colorectal cancer can be a safe, easily accessible and promising therapeutic drug. In the future, more comprehensive and multi-center supported clinical studies are needed to elucidate the mechanism of action of erianin on colorectal cancer cells.

Kaynakça

  • An BC, Hong S, Park HJ, vd. Anti-Colorectal Cancer Effects of Probiotic-Derived p8 Protein. Genes 2019; 10(8). doi:10.3390/genes10080624
  • Weng W, Goel A. Curcumin and colorectal cancer: An update and current perspective on this natural medicine. Seminars in Cancer Biology 2020. doi:10.1016/j.semcancer.2020.02.011
  • Huang X mei, Yang Z jie, Xie Q, Zhang Z kang, Zhang H, Ma J ying. Natural products for treating colorectal cancer: A mechanistic review. Biomedicine and Pharmacotherapy 2019; 117: 109142. doi:10.1016/j.biopha.2019.109142
  • Chung SS, Dutta P, Chard N, vd. A novel curcumin analog inhibits canonical and non-canonical functions of telomerase through STAT3 and NF-κB inactivation in colorectal cancer cells. Oncotarget 2019; 10(44): 4516–4531. doi:10.18632/oncotarget.27000
  • Gamage CDB, Park SY, Yang Y, vd. Deoxypodophyllotoxin exerts anti-cancer effects on colorectal cancer cells through induction of apoptosis and suppression of tumorigenesis. International Journal of Molecular Sciences 2019; 20(11): 2612. doi:10.3390/ijms20112612
  • Wang S, Wang L, Zhou Z, vd. Leucovorin Enhances the Anti-cancer Effect of Bortezomib in Colorectal Cancer Cells. Scientific Reports 2017; 7(1): 682 doi:10.1038/s41598-017-00839-9
  • Wang X, Li T. Ropivacaine inhibits the proliferation and migration of colorectal cancer cells through ITGB1. Bioengineered 2021; 12(1): 44–53. doi:10.1080/21655979.2020.1857120
  • Hlosrichok A, Sumkhemthong S, Sritularak B, Chanvorachote P, Chaotham C. A bibenzyl from Dendrobium ellipsophyllum induces apoptosis in human lung cancer cells. Journal of Natural Medicines 2018; 72(3): 615–625. doi:10.1007/s11418-018-1186-x
  • Guo Z, Zhou Y, Yang J, Shao X. Dendrobium candidum extract inhibits proliferation and induces apoptosis of liver cancer cells by inactivating Wnt/β-catenin signaling pathway. Biomedicine and Pharmacotherapy 2019; 110: 371–379. doi:10.1016/j.biopha.2018.11.149
  • Yang L, Hu Y, Zhou G, Chen Q, Song Z. Erianin suppresses hepatocellular carcinoma cells through down-regulation of PI3K/AKT, p38 and ERK MAPK signaling pathways. Bioscience reports 2020; 40(7): BSR20193137. doi:10.1042/BSR20193137
  • Li M, He Y, Peng C, Xie X, Hu G. Erianin inhibits human cervical cancer cell through regulation of tumor protein p53 via the extracellular signal-regulated kinase signaling pathway. Oncology Letters 2018; 16(4): 5006–5012. doi:10.3892/ol.2018.9267
  • Zhu Q, Sheng Y, Li W, vd. Erianin, a novel dibenzyl compound in Dendrobium extract, inhibits bladder cancer cell growth via the mitochondrial apoptosis and JNK pathways. Toxicology and Applied Pharmacology 2019; 371: 41–54. doi:10.1016/j.taap.2019.03.027
  • Wang H, Zhang T, Sun W, vd. Erianin induces G2/M-phase arrest, apoptosis, and autophagy via the ROS/JNK signaling pathway in human osteosarcoma cells in vitro and in vivo. Cell Death and Disease 2016; 7(6): e2247. doi:10.1038/cddis.2016.138
  • Chen P, Wu Q, Feng J, vd. Erianin, a novel dibenzyl compound in Dendrobium extract, inhibits lung cancer cell growth and migration via calcium/calmodulin-dependent ferroptosis. Signal Transduction and Targeted Therapy. 2020; 5(1): 51. doi:10.1038/s41392-020-0149-3
  • Sun J, Fu X, Wang Y, vd. Erianin inhibits the proliferation of T47D cells by inhibiting cell cycles, inducing apoptosis and suppressing migration. American journal of translational research. 2016;8(7):3077–3086.
  • Rajendran V, Jain MV. In vitro tumorigenic assay: colony forming assay for cancer stem cells. Içinde: Methods in Molecular Biology 2018; 1692: 89–95. doi:10.1007/978-1-4939-7401-6_8
  • Franken NAP, Rodermond HM, Stap J, Haveman J, van Bree C. Clonogenic assay of cells in vitro. Nature Protocols 2006; 1(5): 2315–2319. doi:10.1038/nprot.2006.339
  • Brenner H, Kloor M, Pox CP. Colorectal cancer. The Lancet 2014; 383: 1490–1502. doi:10.1016/S0140-6736(13)61649-9
  • Ruiz de Porras V, Layos L, Martínez-Balibrea E. Curcumin: A therapeutic strategy for colorectal cancer? Seminars in Cancer Biology 2021; 73: 321–330. doi:10.1016/j.semcancer.2020.09.004
  • Khan I, Mahfooz S, Ansari IA. Antiproliferative and Apoptotic Properties of Andrographolide Against Human Colon Cancer DLD1 Cell Line. Endocrine, Metabolic & Immune Disorders - Drug Targets 2019; 20(6): 930–942. doi:10.2174/1871530319666191125111920
  • Liu YT, Hsieh MJ, Lin JT, vd. Erianin induces cell apoptosis through ERK pathway in human nasopharyngeal carcinoma. Biomedicine and Pharmacotherapy 2019; 111: 262–269. doi:10.1016/j.biopha.2018.12.081
  • Zhu Q, Sheng Y, Li W, vd. Erianin, a novel dibenzyl compound in Dendrobium extract, inhibits bladder cancer cell growth via the mitochondrial apoptosis and JNK pathways. Toxicology and Applied Pharmacology 2019; 371: 41–54. doi:10.1016/j.taap.2019.03.027
  • Cho JG, Lim KH, Park SG. MED28 increases the colony-forming ability of breast cancer cells by stabilizing the ZNF224 protein upon DNA damage. Oncology Letters 2018; 15(3): 3147–3154. doi:10.3892/ol.2017.7718

Ayrıntılar

Birincil Dil Türkçe
Konular Tıbbi Araştırmalar Deneysel
Bölüm Özgün Araştırma Makaleleri
Yazarlar

Sema SERTER KOÇOĞLU (Sorumlu Yazar)
Balıkesir Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Balıkesir.
0000-0002-3180-4007
Türkiye


Levent ELMAS
Bakırçay Üniversitesi Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, İzmir.
0000-0002-6865-6466
Türkiye


Mücahit SEÇME
Pamukkale Üniversitesi Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Denizli.
0000-0002-2084-760X
Türkiye

Yayımlanma Tarihi 1 Ağustos 2021
Yayınlandığı Sayı Yıl 2021, Cilt 47, Sayı 2

Kaynak Göster

Bibtex @araştırma makalesi { uutfd917751, journal = {Uludağ Üniversitesi Tıp Fakültesi Dergisi}, issn = {1300-414X}, eissn = {2645-9027}, address = {Uludağ Üniversitesi Tıp Fakültesi Dekanlığı, Görükle Kampüsü 16059, Nilüfer, BURSA}, publisher = {Bursa Uludağ Üniversitesi}, year = {2021}, volume = {47}, pages = {219 - 223}, doi = {10.32708/uutfd.917751}, title = {Erianinin HT29 Kolorektal Kanser Hücrelerinin Proliferasyonu ve Koloni Oluşumu Üzerine Etkilerinin Araştırılması}, key = {cite}, author = {Serter Koçoğlu, Sema and Elmas, Levent and Seçme, Mücahit} }
APA Serter Koçoğlu, S. , Elmas, L. & Seçme, M. (2021). Erianinin HT29 Kolorektal Kanser Hücrelerinin Proliferasyonu ve Koloni Oluşumu Üzerine Etkilerinin Araştırılması . Uludağ Üniversitesi Tıp Fakültesi Dergisi , 47 (2) , 219-223 . DOI: 10.32708/uutfd.917751
MLA Serter Koçoğlu, S. , Elmas, L. , Seçme, M. "Erianinin HT29 Kolorektal Kanser Hücrelerinin Proliferasyonu ve Koloni Oluşumu Üzerine Etkilerinin Araştırılması" . Uludağ Üniversitesi Tıp Fakültesi Dergisi 47 (2021 ): 219-223 <https://dergipark.org.tr/tr/pub/uutfd/issue/64874/917751>
Chicago Serter Koçoğlu, S. , Elmas, L. , Seçme, M. "Erianinin HT29 Kolorektal Kanser Hücrelerinin Proliferasyonu ve Koloni Oluşumu Üzerine Etkilerinin Araştırılması". Uludağ Üniversitesi Tıp Fakültesi Dergisi 47 (2021 ): 219-223
RIS TY - JOUR T1 - Erianinin HT29 Kolorektal Kanser Hücrelerinin Proliferasyonu ve Koloni Oluşumu Üzerine Etkilerinin Araştırılması AU - Sema Serter Koçoğlu , Levent Elmas , Mücahit Seçme Y1 - 2021 PY - 2021 N1 - doi: 10.32708/uutfd.917751 DO - 10.32708/uutfd.917751 T2 - Uludağ Üniversitesi Tıp Fakültesi Dergisi JF - Journal JO - JOR SP - 219 EP - 223 VL - 47 IS - 2 SN - 1300-414X-2645-9027 M3 - doi: 10.32708/uutfd.917751 UR - https://doi.org/10.32708/uutfd.917751 Y2 - 2021 ER -
EndNote %0 Uludağ Üniversitesi Tıp Fakültesi Dergisi Erianinin HT29 Kolorektal Kanser Hücrelerinin Proliferasyonu ve Koloni Oluşumu Üzerine Etkilerinin Araştırılması %A Sema Serter Koçoğlu , Levent Elmas , Mücahit Seçme %T Erianinin HT29 Kolorektal Kanser Hücrelerinin Proliferasyonu ve Koloni Oluşumu Üzerine Etkilerinin Araştırılması %D 2021 %J Uludağ Üniversitesi Tıp Fakültesi Dergisi %P 1300-414X-2645-9027 %V 47 %N 2 %R doi: 10.32708/uutfd.917751 %U 10.32708/uutfd.917751
ISNAD Serter Koçoğlu, Sema , Elmas, Levent , Seçme, Mücahit . "Erianinin HT29 Kolorektal Kanser Hücrelerinin Proliferasyonu ve Koloni Oluşumu Üzerine Etkilerinin Araştırılması". Uludağ Üniversitesi Tıp Fakültesi Dergisi 47 / 2 (Ağustos 2021): 219-223 . https://doi.org/10.32708/uutfd.917751
AMA Serter Koçoğlu S. , Elmas L. , Seçme M. Erianinin HT29 Kolorektal Kanser Hücrelerinin Proliferasyonu ve Koloni Oluşumu Üzerine Etkilerinin Araştırılması. Uludağ Tıp Derg. 2021; 47(2): 219-223.
Vancouver Serter Koçoğlu S. , Elmas L. , Seçme M. Erianinin HT29 Kolorektal Kanser Hücrelerinin Proliferasyonu ve Koloni Oluşumu Üzerine Etkilerinin Araştırılması. Uludağ Üniversitesi Tıp Fakültesi Dergisi. 2021; 47(2): 219-223.
IEEE S. Serter Koçoğlu , L. Elmas ve M. Seçme , "Erianinin HT29 Kolorektal Kanser Hücrelerinin Proliferasyonu ve Koloni Oluşumu Üzerine Etkilerinin Araştırılması", Uludağ Üniversitesi Tıp Fakültesi Dergisi, c. 47, sayı. 2, ss. 219-223, Ağu. 2021, doi:10.32708/uutfd.917751