Effects of Curcumin on Cell Viability and Apoptosis of Ishikawa Cell Lines

Yıl 2022, Cilt: 48 Sayı: 1, 59 - 64, 01.04.2022

Hatice Kübra Başaloğlu , Çiğdem Yenisey , Mehmet Turgut , Emel Öykü Çetin Uyanıkgil , Yiğit Uyanıkgil

https://doi.org/10.32708/uutfd.1067308

Öz

Curcumin (diferuloylmethane), has been used in alternative medicine for anticancer therapies mainly in Asian countries especially in China. In recent years such evidence suggested that curcumin comprise anti-tumoral natures against some of cancers. Nonetheless, it was little known its anti-tumoral effects and molecular mechanism on endometrial carcinoma. The purpose of this research evaluating anti-tumoral effects of curcumin in endometrial cancer cell lines of Ishikawa. We incubated the Ishikawa cell lines with curcumin at 10, 20, 40, 80 and 100 μM concentrates. We evaluated apoptosis and caspase 3/7 values and cell viability after 24, 48 and 72 hours of incubation. It was found that curcumin inhibited Ishikawa cell viability in vitro. Also, treatment with curcumin for 48 hours, at 10 and 20 μM concentrations apoptosis were highest but 100μM concentrations it was observed that cells were going necrosis instead of apoptosis. On the other hand caspase 3/7 enzymes results were the highest at 80 μM concentration of curcumin. We think that curcumin affects different apoptotic pathways in the Ishikawa cell line.

Anahtar Kelimeler

Apoptosis, Curcumin, Ishikawa cell line

Kurkuminin Ishikawa Hücre Hattında Canlılık ve Apoptoz Üzerindeki Etkileri

Öz

Kurkumin (diferuloilmetan), başta Çin olmak üzere Asya ülkelerinde kanser tedavisi için alternatif tıpta kullanılmaktadır. Son yıllarda, kurkuminin bazı kanser tiplerine karşı doğal bir antitümöral ajan olduğu öne sürülmektedir. Bu araştırmanın amacı, Ishikawa endometriyal kanser hücre hattı üzerinde kurkuminin antitümöral etkilerini değerlendirmektir. Ishikawa hücreleri 10, 20, 40, 80 ve 100 μM konsantrasyonlarda kurkumin ile inkübe edildi. 24, 48 ve 72 saatlik inkübasyon sonrası apoptoz, kaspaz 3/7 değerleri ve hücre canlılığı değerlendirildi. Kurkuminin in vitro olarak, Ishikawa hücre canlılığını üzerinde inhibe edici etki gösterdiği saptandı. Ayrıca 48 saat kurkumin ile 10 ve 20 μM konsantrasyonlarda apoptoz en yüksek düzeyde iken, 100μM konsantrasyonlarda hücrelerin apoptoz yerine nekroza gittiği gözlendi. Öte yandan kaspaz 3/7 enzim sonuçları, 80 μM kurkumin konsantrasyonunda en yüksek düzeyde saptandı. Kurkumin, Ishikawa hücre hattı üzerinde farklı hücre ölüm yolağını etkilemektedir.

Anahtar Kelimeler

Apoptoz, kurkumin, Ishikawa hücre hattı

Kaynakça

• Chen Q, Gao Q, Chen K, Wang Y, Chen L, Li X. Curcumin suppresses migration and invasion of human endometrial carcinoma cells. Oncol Lett. 2015;10:1297-1302.
• Felix AS, Yang HP, Bell DW, Sherman ME. Epidemiology of endometrial carcinoma: etiologic importance of hormonal and metabolic influences. Adv Exp Med Biol. 2017;943:3-46.
• Kumar A, Kumar Sirohi V, Anum F, Singh PK, Gupta K, Gupta D, et al. Enhanced apoptosis, survivin down-regulation and assisted immunochemotherapy by curcumin loaded amphiphilic mixed micelles for subjugating endometrial cancer. Nanomedicine: Nanotechnology, Biology and Medicine 2017;13:1953-63.
• Xu H, Gong Z, Zhou S, Yang S, Wang D, Chen X, Wu J, Liu L, Zhong S, Zhao J, Tang J. Liposomal curcumin targeting endometrial cancer through the NF-κB pathway. Cell Physiol Biochem. 2018;48:569-82.
• Khoury E, Matar R, TTouma T. Curcumin and endometrial carcinoma: an old spice as a novel agent. International Journal of Women's Health 2019;11:249-256.
• Başaran D, Salman MC, Yüce K. Endometrial cancer: management of serous and clear cell histologies. Turkiye Klinikleri J Gynecol Obst-Special Topics. 2014;7:75-83
• Dempe JS, Pfeiffer E, Grimm AS, Metzler M. Metabolism of curcumin and induction of mitotic catastrophe in human cancer cells. Mol Nutr Food Res. 2008;52:1074-81.
• Shanmugam MK, Rane G, Kanchi MM, Arfuso F, Chinnathambi A, Zayed ME, Alharbi SA, Tan BK, Kumar AP, Sethi G. The multifaceted role of curcumin in cancer prevention and treatment. Molecules. 2015;20:2728-69.
• Shen L, Ji HF. The pharmacology of curcumin: is it the degradation products? Trends Mol Med. 2012;18:138-44.
• Ravindran J, Prasad S, Aggarw BB. Curcumin and cancer cells: how many ways can curry kill tumor cells selectively? The AAPS Journal 2009;11:495-510.
• Shehzad A, Qureshi M, Anwar MN, Lee YS. Multifunctional curcumin mediate multitherapeutic effects. J Food Sci. 2017;82:2006-15.
• Nishida M. The Ishikawa cells from birth to the present. Human Cell 2002 15:3. 2002;15(3):104–17.
• Naciff JM, Khambatta ZS, Carr GJ, Tiesman JP, Singleton DW, Khan SA, et al. Dose- and Time-Dependent Transcriptional Response of Ishikawa Cells Exposed to Genistein. Toxicological Sciences. 2016 May 1;151(1):71.
• Albitar L, Pickett G, Morgan M, Wilken JA, Maihle NJ, Leslie KK. EGFR isoforms and gene regulation in human endometrial cancer cells. Molecular Cancer. 2010 Jun 25;9:166.
• Maheshwari RK, Singh AK, Gaddipati J, Srimal RC. Multiple biological activities of curcumin: a short review. Life Sci. 2006;27;78:2081-7.
• Kim HJ, Park SY, Park OJ, Kim YM. Curcumin suppresses migration and proliferation of Hep3B hepatocarcinoma cells through inhibition of the Wnt signaling pathway. Mol Med Rep 2013;8:282–6.
• Ono M, Higuchi T, Takeshima M, Chen C, Nakano S.Antiproliferativeandapoptosis-inducing activity of curcumin against human gallbladder adenocarcinoma cells. Anticancer Res 2013;33:1861–6.
• Youns M, Fathy GM. Upregulation of extrinsic apoptotic pathway in curcumin-mediated antiproliferative effect on human pancreatic carcinogenesis. J Cell Biochem 2013;114:2654–65.
• Xu X, Qin J, Liu W. Curcumin inhibits the invasion of thyroid cancer cells via down-regulation of PI3K/Akt signaling pathway. Gene 2014;546:226–32.
• Sirohi VK, Popli P, Sankhwar P, Kaushal JB, Gupta K, Manohar M, Dwivedi A. Curcumin exhibits anti-tumor effect and attenuates cellular migration via Slit-2 mediated down-regulation of SDF-1 and CXCR4 in endometrial adenocarcinoma cells. J Nutr Biochem. 2017;44:60-70.
• Feng W, Yang CX, Zhang L, Fang Y, Yan M. Curcumin promotes the apoptosis of human endometrial carcinoma cells by downregulating the expression of androgen receptor through Wnt signal pathway. Eur J Gynaecol Oncol. 2014;35:718-23.
• Sun M, Yu F, Gong M, Fan G, Liu C. Effects of curcumin on the role of MMP-2 in endometrial cancer cell proliferation and invasion. Eur Rev Med Pharmacol Sci. 2018;22:5033–5041.
• Tierney BJ, McCann GA, Naidu S, et al. Aberrantly activated pSTAT3-Ser727 in human endometrial cancer is suppressed by HO-3867, a novel STAT3 inhibitor. Gynecol Oncol. 2014;135:133–141.
• Lin SS, Huang HP, Yang JS, et al. DNA damage andendoplasmic reticulum stress mediated curcumin-induced cellcycle arrest and apoptosis in human lung carcinoma A-549 cells through the activation caspases cascade- and mitochondrial-dependent pathway. Cancer Lett. 2008;272:355.
• Thayyullathil F, Chathoth S, Hago A, Patel M, Galadari S. Rapid reactive oxygen species (ROS) generation induced by curcumin leads to caspase-dependent and -independent apoptosis in L929cells. Free Radic Biol Med. 2008;45:1403–1412.