Bioactive and Anti-carcinogenic Properties of Kombucha Prepared with Aronia Melanocarpa Juice

Yıl 2023, Cilt: 4 Sayı: 4, 198 - 206, 30.12.2023

Zeynep Çalışkan Elif Yıldız Metin Güldaş Ozan Gürbüz

Öz

Aim: Polyphenolics derived from Aronia Melanocarpa
may have beneficial effects in reducing the risk of several diseases, such as diabetes, cardiovascular diseases, and
cancer. Kombucha is a symbiotic system comprising cultures of bacteria and yeasts known as SCOBY. It is prepared by fermenting sugar and tea leaves. In this study, we
aimed to enhance the beneficial aspects of Kombucha by
preparing it using aronia juice as a fermentation substrate.
Methods: The total phenolic content was measured quantitatively through the Folin-Ciocalteu method, with Gallic
acid as the standard. The antioxidant capacity was determined using the ABTS and DPPH methods. Cell viability
in colon cancer and normal fibroblasts was determined
using the MTT assay.
Results: The mean values of pH and total acidity of aronia
juice used in Kombucha production were determined as
5.24±0.00 and 0.45±0.05, respectively. The total phenolic content was highest in the sample containing green tea
and aronia juice. The antioxidant capacity of the sample
containing green tea and aronia juice had the highest values in extractable (TEACABTS: 13.39 µmol Trolox/mL),
hydrolyzable (TEACDPPH: 15.15 µmol Trolox/mL) and
bioaccessible phenolics (TEACABTS: 4.77 µmol Trolox/
mL). However, we found a decline by about 56% in the
viability of colon cancer cells (HT-29) after treatment with
the Kombucha sample containing green tea and aronia
juice.
Conclusion: Enrichment of Kombucha with aronia juice
is a good alternative for producing new fermented drinks
with high antioxidant capacity. Kombucha enriched with
aronia juice has high total phenolic content and antioxi dant capacity. Besides, it may be considered in the context
of colon cancer prevention.

Anahtar Kelimeler

Aronia melanocarpa, Kombucha, anti-carcinogenic effect, antioxidant capacity, bioaccessibility

Kaynakça

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