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Evaluation of nuclear condensation and spermatozoa morphology at the level of light microscope in different semen parameters

Yıl 2009, Cilt 40, Sayı 3, 111 - 117, 01.04.2009

Öz

Aim: It was aimed to evaluate sperm DNA decondansation of oligoasthenoospermic and teratozoospermic patients at the level of light microscope. Material and Metods: Seminal plazma of oligoasthenoospermic (n: 20) and teratozoospermic (n: 25) samples were washed twice with PBS and then smear was prepared, fixation was done with metil alcohol. Staining was done with; heamatoxylin-eosin, toluidine blue, Giemsa and Wright stains. Under light microscopy 200 sperm was counted, condansated and decondansated ones were counted. Findings: Between four stains condensation/decondensation selection was done more easier with haematoxylin-eosin and toluidine stains. Statistical analyses did not show any difference on condensation evaluation between stains. There was no any significant difference for condensation ratio values between groups. Also comparasion between oligoasthenoospermic and teratozoospermic samples done with stains showed no any difference. But when safety area of graphic was considered (especially when teratozoospermic was compared to oligoasthenoospermic) toluidine stain showed a homogen appearance. Condensated sperm ratio was higher in teratozoospermic samples. Results: As a result toluidine blue is the most trustable stainjbr condensation evaluation. There was no any difference between four stains between oligoasthenoospermic and teratozoospermic samples. For intracytoplasmic sperm injection teratozoospermic samples are more reliable than oligoasthenoospermic samples.

Kaynakça

  • l.SpiraA. Epidemiology ofHuman Reproduction. Hum Reprod. 1986; 1: 111-115
  • 2.Anafarta K, Bedük Y, Arıkan N. Temel Üroloji, 3.baskı. Güneş Kitabevi 2007; 967-990
  • 3.Van Steirteghem AC, Nagy PZ, Joris H, Liu J, Staessen C, Smitz J. High fertilization and implantation rates after intracytoplasmic spermatozoa injection. Hum Reprod 8 1993; 1061-1066
  • 4.RS, Althouse GC. Determining sample size for the morphological assessment of spermatozoa. Theriogenology 2004;61: 691-703
  • 5.World Health Organization. WHO Laboratory Manual for the Examination on Human Semen and Spermatozoa-Cervical Mucus Interaction. 4th ed. Cambridge, Cambridge University Press, 1999; 76: 4-33
  • 6.Delilbaşı L.A'dan Z'ye Tüp Bebek Laboratuar. Veri medikal Yayıncıhk.2008; 62
  • 7.Rajvi H Mehta, Sanjay Makwana, Geetha M Ranga, RJ Srinivasan, SS Virk. Prevalences of oligozoospermia and azoospermia in male partners of infertile couples from different parts of India. Asian JAndrol 2006; 8 (1): 89-93
  • 8.JunqueiraLC, Carneiro J, RO Kelley. Çev Ed Aytekin Y. Temel Histoloji. Barış Kitabevi İstanbul, 1998; S: 407- 422
  • 9.35.Clermont Y. Kinetics ofspermatogenesis in: 198-236
  • 10.Parks JE, Lee DR, Huang S, Kaproth MT. Prospects for spermatogenesis in vitro. Theriogenology, 2003;59:73-86
  • 11.Palermo G, Joris H, Devroey P, Van Steirteghem AC. Pregnancies after intracytoplasmic Injection of a single spermatozoa into an oocyte. Lancet 1992.; 340:17-18
  • 12.Brugh VM, Matschke HM, Lipshultz LI. Male factor infertility: Evaluation and management. Endocrinol MedClin North Am 2004; 88: 367-385
  • 13.Coetzee K, Kruger TF, Lombard CJ. Predictive valueof normal spermatozoa morphology a structured review. Hum Reprod 1998; Update 4: 73-82
  • 14.Ombelet W, Pollet H, Bosman E, Vereecken A. Results of aquestionnaire on spermatozoa morphology assessment. Hum Reprod 1997; 12: 1015-1020
  • 15.Bernard Jegou, Charles Pineau, Jorna Toppari. Spermatogenesis in vitro in mammals. In Assisted Reproductive Technology. Jonge CD, Barratt CLR, eds. CambridgeUniversity Press: Cambridge. 2002;3-25
  • 16.Brugh VM, Matschke HM, LipshultzLI. Male factor infertility: Evaluation and management. Endocrinol Med Clin North Am 2004; 88:367-385 17.Anthony V Hirsh. The investigation and therapeutic options for infertile men presenting in assisted conception units. In: In vitro fertilization and assisted reproduction: the Bourn Hall guide to clinical and laboratory practice. Second ed, Brinsden PR, ed. NewYork: Parthenon Publishing. 1999; 27-52
  • 18.MenkveldR, StanderFSH, Kotze TJvW, Kruger Tf van ZylJA. The evaluation of morphological characteristics of human spermatozoa according to stricter criteria. Hum Reprod 1990; 5: 586-92
  • 19.Garcia Herreros M, Aparicio IM, Baron FJ, Garcia Marin LJ, Gil MC. Standardization of sample preparation, staining and sampling methods for automated spermatozoa head morphometry analysis of boar spermatozoa. Int JAndrol. 2006 Oct;29(5):553-63
  • 20.C Soler, B Gadea, AJ Soler, MR Fernandez Santos, MC Esteso, J Nunez, PN Moreira, M Nunez, R Gutierrez, M Sancho and JJ Gadre. Comparison of three different staining methods for the assessment of epididymal red deer spermatozoamorphometry by computerized analysis withlSAS , 15 September 2005, Pages 1236-1243
  • 21.MC Sardor, MI Carretero and DM Neild. Evaluation of stallion spermatozoa DNA alterations during cryopreservation using toluidine blue., September 2008; Pages: 349-350
  • 22.Reda Z. Mahfouz MD, Rakesh K. Sharma Ph D, Tamer M Said MD, Juris Erenpreiss M D and Ashok Agarwal PhD, HCLD. Association of spermatozoa apoptosis and DNA ploidy with spermatozoachromatin quality in human spermatozoa 23.Beletti ME, Mello ML. Comparison between the toluidine blue stain and the Feulgen reaction for evaluation of rabbit spermatozoa chromatin condensation and their relationship with spermatozoa morphology. Theriogenology. 2004Aug;62(3-4):398-402
  • 24.Krzanowska H. Toluidine blue staining reveals changes in chromatin stabilization of mouse spermatozoa during epididymal maturation and penetration of ova. 1982 Jan;64(l):97-101
  • 25.Kutvölgyi G, Stefler J, Kovacs A. Viability and acrosome staining of stallion spermatozoa by Chicago sky blue and Giemsa. Biotech Histochem. 2007Feb;82(l):45
  • 26.CM Tartaglione and MN Ritta. Prognostic value of spermatological parameters as predictors of in vitro fertility of frozen-thawed bull semen , 1 October 2004;Pages: 1245-1252
  • 27.Jacob Ashkenazi, Raoul Orvieto, Ruth Gold-Deutch, Dov Feldberg, Dov Dicker, Isachar Voliovitch and Zion Ben-Rafael The impact of woman's age and spermatozoa parameters on fertilization rates in IVF cycles , June 1996; Pages: 155-159
  • 28.Eggert-Kruse W, Schwarz H, Rohr G, Demirakca T, Tilgen W, Runnebaum B. Spermatozoa morphology assessment using strict criteria and male fertility under in-vivo conditions of conception
  • 1996 Jan; 11(1): 139-46
  • 29.Kovacs A, Foote RH. Viability and acrosome staining of bull, boar and rabbit spermatozoa. 1992 May;67(3):119-24
  • 30.Kretser DM, Baker HWG. Human Infertility: The male factor. In Reproductive Endocrinology,Surgery and Technology. Adashi EY, Rock JA, Rosenwaks Z, eds.Lippincott-Raven.New York, 1996; 2031-61.

Farklı semen paramerrelerinde ışık mikroskobu düzeyinde spermatozoa morfolojisi ve nükleer kondansasyon değerlendirmesi

Yıl 2009, Cilt 40, Sayı 3, 111 - 117, 01.04.2009

Öz

Amaç: Oligozoospermili ve teratozoospermili hastalarda Işık Mikroskobu düzeyinde sperm morfolojisi ve sperm DNA kondansasyonunun değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem: oligozoospermili (n: 20) ve teratozoospermili (n: 25) hastalardan alınan seminal plazma örnekleri PBS ile 2 kez yıkandı ve smear hazırlandı, metil alkol ile fikzasyonu hazırlandı. Hematoksilen-Eosin, Toluidin-Blue, Giemsa ve Wright özel histolojik boyaları ile boyandı. Işık mikroskobunda 200 sperm hücresi sayılıp kondanse ve dekondanse başa sahip olan spermatozoalar belirlendi. Bulgular: Bu dört boya ile yapılan yaymalarda kondanse spermler, Hematoksüen-Eozin ve Toluidin Blue boyası ile boyanmış preperatlarda daha kolay ve net görüldü. İstatistiksel olarak dört boya ile kondansasyon değerlendirmesinde hasta grupları arasında fark olmadığı görüldü. Ayrıca oligozoospermili ve teratozoospermili hasta grupları için boyalar kendi içinde karşılaştırıldığında fark olmadığı görüldü. Ancak güven aralığı grafiğine bakıldığında; teratozoospermili hastaların oligozoospermili hastalara göre özellikle de Toluidin blue ile boyanmış grubun belirgin ölçüde homojen dağılım göstermesi; teratozoospermili hasta grubunun daha güvenilir kondanse sperm sağladığını gösterdi Sonuç: Sperm kondansasyon değerlendirmesi için toluidin blue boyası en güvenilir boyalardan bindir. Oligozoospermili ve teratozoospermili hastalar arasında ve bu dört boya arasında sperm nükleer kondansasyonunda fark yoktur. Ancak intrastoplazmik sperm injeksiyonu için kondanse sperm seçimi yapılırken oligozoospermili hastalara göre teratozoospermili hastalar daha güvenilir sonuç verebilir.

Kaynakça

  • l.SpiraA. Epidemiology ofHuman Reproduction. Hum Reprod. 1986; 1: 111-115
  • 2.Anafarta K, Bedük Y, Arıkan N. Temel Üroloji, 3.baskı. Güneş Kitabevi 2007; 967-990
  • 3.Van Steirteghem AC, Nagy PZ, Joris H, Liu J, Staessen C, Smitz J. High fertilization and implantation rates after intracytoplasmic spermatozoa injection. Hum Reprod 8 1993; 1061-1066
  • 4.RS, Althouse GC. Determining sample size for the morphological assessment of spermatozoa. Theriogenology 2004;61: 691-703
  • 5.World Health Organization. WHO Laboratory Manual for the Examination on Human Semen and Spermatozoa-Cervical Mucus Interaction. 4th ed. Cambridge, Cambridge University Press, 1999; 76: 4-33
  • 6.Delilbaşı L.A'dan Z'ye Tüp Bebek Laboratuar. Veri medikal Yayıncıhk.2008; 62
  • 7.Rajvi H Mehta, Sanjay Makwana, Geetha M Ranga, RJ Srinivasan, SS Virk. Prevalences of oligozoospermia and azoospermia in male partners of infertile couples from different parts of India. Asian JAndrol 2006; 8 (1): 89-93
  • 8.JunqueiraLC, Carneiro J, RO Kelley. Çev Ed Aytekin Y. Temel Histoloji. Barış Kitabevi İstanbul, 1998; S: 407- 422
  • 9.35.Clermont Y. Kinetics ofspermatogenesis in: 198-236
  • 10.Parks JE, Lee DR, Huang S, Kaproth MT. Prospects for spermatogenesis in vitro. Theriogenology, 2003;59:73-86
  • 11.Palermo G, Joris H, Devroey P, Van Steirteghem AC. Pregnancies after intracytoplasmic Injection of a single spermatozoa into an oocyte. Lancet 1992.; 340:17-18
  • 12.Brugh VM, Matschke HM, Lipshultz LI. Male factor infertility: Evaluation and management. Endocrinol MedClin North Am 2004; 88: 367-385
  • 13.Coetzee K, Kruger TF, Lombard CJ. Predictive valueof normal spermatozoa morphology a structured review. Hum Reprod 1998; Update 4: 73-82
  • 14.Ombelet W, Pollet H, Bosman E, Vereecken A. Results of aquestionnaire on spermatozoa morphology assessment. Hum Reprod 1997; 12: 1015-1020
  • 15.Bernard Jegou, Charles Pineau, Jorna Toppari. Spermatogenesis in vitro in mammals. In Assisted Reproductive Technology. Jonge CD, Barratt CLR, eds. CambridgeUniversity Press: Cambridge. 2002;3-25
  • 16.Brugh VM, Matschke HM, LipshultzLI. Male factor infertility: Evaluation and management. Endocrinol Med Clin North Am 2004; 88:367-385 17.Anthony V Hirsh. The investigation and therapeutic options for infertile men presenting in assisted conception units. In: In vitro fertilization and assisted reproduction: the Bourn Hall guide to clinical and laboratory practice. Second ed, Brinsden PR, ed. NewYork: Parthenon Publishing. 1999; 27-52
  • 18.MenkveldR, StanderFSH, Kotze TJvW, Kruger Tf van ZylJA. The evaluation of morphological characteristics of human spermatozoa according to stricter criteria. Hum Reprod 1990; 5: 586-92
  • 19.Garcia Herreros M, Aparicio IM, Baron FJ, Garcia Marin LJ, Gil MC. Standardization of sample preparation, staining and sampling methods for automated spermatozoa head morphometry analysis of boar spermatozoa. Int JAndrol. 2006 Oct;29(5):553-63
  • 20.C Soler, B Gadea, AJ Soler, MR Fernandez Santos, MC Esteso, J Nunez, PN Moreira, M Nunez, R Gutierrez, M Sancho and JJ Gadre. Comparison of three different staining methods for the assessment of epididymal red deer spermatozoamorphometry by computerized analysis withlSAS , 15 September 2005, Pages 1236-1243
  • 21.MC Sardor, MI Carretero and DM Neild. Evaluation of stallion spermatozoa DNA alterations during cryopreservation using toluidine blue., September 2008; Pages: 349-350
  • 22.Reda Z. Mahfouz MD, Rakesh K. Sharma Ph D, Tamer M Said MD, Juris Erenpreiss M D and Ashok Agarwal PhD, HCLD. Association of spermatozoa apoptosis and DNA ploidy with spermatozoachromatin quality in human spermatozoa 23.Beletti ME, Mello ML. Comparison between the toluidine blue stain and the Feulgen reaction for evaluation of rabbit spermatozoa chromatin condensation and their relationship with spermatozoa morphology. Theriogenology. 2004Aug;62(3-4):398-402
  • 24.Krzanowska H. Toluidine blue staining reveals changes in chromatin stabilization of mouse spermatozoa during epididymal maturation and penetration of ova. 1982 Jan;64(l):97-101
  • 25.Kutvölgyi G, Stefler J, Kovacs A. Viability and acrosome staining of stallion spermatozoa by Chicago sky blue and Giemsa. Biotech Histochem. 2007Feb;82(l):45
  • 26.CM Tartaglione and MN Ritta. Prognostic value of spermatological parameters as predictors of in vitro fertility of frozen-thawed bull semen , 1 October 2004;Pages: 1245-1252
  • 27.Jacob Ashkenazi, Raoul Orvieto, Ruth Gold-Deutch, Dov Feldberg, Dov Dicker, Isachar Voliovitch and Zion Ben-Rafael The impact of woman's age and spermatozoa parameters on fertilization rates in IVF cycles , June 1996; Pages: 155-159
  • 28.Eggert-Kruse W, Schwarz H, Rohr G, Demirakca T, Tilgen W, Runnebaum B. Spermatozoa morphology assessment using strict criteria and male fertility under in-vivo conditions of conception
  • 1996 Jan; 11(1): 139-46
  • 29.Kovacs A, Foote RH. Viability and acrosome staining of bull, boar and rabbit spermatozoa. 1992 May;67(3):119-24
  • 30.Kretser DM, Baker HWG. Human Infertility: The male factor. In Reproductive Endocrinology,Surgery and Technology. Adashi EY, Rock JA, Rosenwaks Z, eds.Lippincott-Raven.New York, 1996; 2031-61.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Makaleler
Yazarlar

Emine AKSOY Bu kişi benim


Tahsin Murad AKTAN Bu kişi benim


Selçuk DUMAN Bu kişi benim


Duygu DURSUNOĞLU Bu kişi benim


Gökhan CÜCE Bu kişi benim

Yayımlanma Tarihi 1 Nisan 2009
Yayınlandığı Sayı Yıl 2009, Cilt 40, Sayı 3

Kaynak Göster

Bibtex @ { zktipb236948, journal = {Zeynep Kamil Tıp Bülteni}, issn = {1300-7971}, eissn = {2148-4864}, address = {}, publisher = {Zeynep Kamil Kadın ve Çocuk Hastalıkları EAH}, year = {2009}, volume = {40}, number = {3}, pages = {111 - 117}, doi = {10.16948/zktb.51979}, title = {Farklı semen paramerrelerinde ışık mikroskobu düzeyinde spermatozoa morfolojisi ve nükleer kondansasyon değerlendirmesi}, key = {cite}, author = {Aksoy, Emine and Aktan, Tahsin Murad and Duman, Selçuk and Dursunoğlu, Duygu and Cüce, Gökhan} }
APA Aksoy, E. , Aktan, T. M. , Duman, S. , Dursunoğlu, D. & Cüce, G. (2009). Farklı semen paramerrelerinde ışık mikroskobu düzeyinde spermatozoa morfolojisi ve nükleer kondansasyon değerlendirmesi . Zeynep Kamil Tıp Bülteni , 40 (3) , 111-117 . Retrieved from https://dergipark.org.tr/tr/pub/zktipb/issue/22070/236948
MLA Aksoy, E. , Aktan, T. M. , Duman, S. , Dursunoğlu, D. , Cüce, G. "Farklı semen paramerrelerinde ışık mikroskobu düzeyinde spermatozoa morfolojisi ve nükleer kondansasyon değerlendirmesi" . Zeynep Kamil Tıp Bülteni 40 (2009 ): 111-117 <https://dergipark.org.tr/tr/pub/zktipb/issue/22070/236948>
Chicago Aksoy, E. , Aktan, T. M. , Duman, S. , Dursunoğlu, D. , Cüce, G. "Farklı semen paramerrelerinde ışık mikroskobu düzeyinde spermatozoa morfolojisi ve nükleer kondansasyon değerlendirmesi". Zeynep Kamil Tıp Bülteni 40 (2009 ): 111-117
RIS TY - JOUR T1 - Farklı semen paramerrelerinde ışık mikroskobu düzeyinde spermatozoa morfolojisi ve nükleer kondansasyon değerlendirmesi AU - EmineAksoy, Tahsin MuradAktan, SelçukDuman, DuyguDursunoğlu, GökhanCüce Y1 - 2009 PY - 2009 N1 - DO - T2 - Zeynep Kamil Tıp Bülteni JF - Journal JO - JOR SP - 111 EP - 117 VL - 40 IS - 3 SN - 1300-7971-2148-4864 M3 - UR - Y2 - 2023 ER -
EndNote %0 Zeynep Kamil Tıp Bülteni Farklı semen paramerrelerinde ışık mikroskobu düzeyinde spermatozoa morfolojisi ve nükleer kondansasyon değerlendirmesi %A Emine Aksoy , Tahsin Murad Aktan , Selçuk Duman , Duygu Dursunoğlu , Gökhan Cüce %T Farklı semen paramerrelerinde ışık mikroskobu düzeyinde spermatozoa morfolojisi ve nükleer kondansasyon değerlendirmesi %D 2009 %J Zeynep Kamil Tıp Bülteni %P 1300-7971-2148-4864 %V 40 %N 3 %R %U
ISNAD Aksoy, Emine , Aktan, Tahsin Murad , Duman, Selçuk , Dursunoğlu, Duygu , Cüce, Gökhan . "Farklı semen paramerrelerinde ışık mikroskobu düzeyinde spermatozoa morfolojisi ve nükleer kondansasyon değerlendirmesi". Zeynep Kamil Tıp Bülteni 40 / 3 (Nisan 2009): 111-117 .
AMA Aksoy E. , Aktan T. M. , Duman S. , Dursunoğlu D. , Cüce G. Farklı semen paramerrelerinde ışık mikroskobu düzeyinde spermatozoa morfolojisi ve nükleer kondansasyon değerlendirmesi. Zeynep Kamil Tıp Bülteni. 2009; 40(3): 111-117.
Vancouver Aksoy E. , Aktan T. M. , Duman S. , Dursunoğlu D. , Cüce G. Farklı semen paramerrelerinde ışık mikroskobu düzeyinde spermatozoa morfolojisi ve nükleer kondansasyon değerlendirmesi. Zeynep Kamil Tıp Bülteni. 2009; 40(3): 111-117.
IEEE E. Aksoy , T. M. Aktan , S. Duman , D. Dursunoğlu ve G. Cüce , "Farklı semen paramerrelerinde ışık mikroskobu düzeyinde spermatozoa morfolojisi ve nükleer kondansasyon değerlendirmesi", Zeynep Kamil Tıp Bülteni, c. 40, sayı. 3, ss. 111-117, Nis. 2009, doi:10.16948/zktb.51979