Metal Resistance Properties of Lactobacillus plantarum Strains Isolated from Some Fermented Foods

Year 2016, Volume: 14 Issue: 4, 393 - 397, 01.12.2016

Burcu Özel Halil İbrahim Kaya Ömer Şimşek

Abstract

Lactic acid bacteria constituting the natural microflora of fermented foods may expose to metal stresses during food production. This could interfere with the fermentation by influencing the metabolic activity of LAB adversely. In this study, the resistance to Cu, Cd, Zn, Fe, Co, Al and Cr of 43 Lactobacillus plantarum strains isolated and identified from various sourdough, sucuk, white cheese and pickle samples as well as the existence of resistance genes were determined. L. plantarum strains were more resistant to Fe and Co than other metals studied. Also, Cu-transport ATPase was detected in the genome of L. plantarum PFC19, PFC22, PFC33 and PFC36 whereas Cd-transport ATPase was found at L. plantarum PFC7 and PFC36. Results showed that L. plantarum PFC7, PFC19, PFC22, PFC33 and PFC36 have potential as starter culture in fermented foods against possible metal stress due to their genetically based metal resistance properties

Keywords

Lactic acid bacteria, L. plantarum, Metal resistance, Fermented foods

Bazı Fermente Gıdalardan İzole Edilen Lactobacillus plantarum Suşlarının Metal Dirençlilik Özellikleri

Abstract

Fermente gıdaların doğal mikroflorasını oluşturan laktik asit bakterileri LAB gıda üretiminde metal stresine maruz kalabilmektedir. Bu durum, LAB’lerin metabolik aktivitelerini olumsuz etkileyerek fermentasyonun duraklamasına neden olur. Bu çalışmada ekşi hamur, sucuk, beyaz peynir ve turşudan izole edilmiş ve tanımlanmış 43 adet Lactobacillus plantarum suşlarının Cu, Cd, Zn, Fe, Co, Al ve Cr metallerine karşı dirençlilik seviyeleri ve suşlarda dirençlilik genlerinin varlığı araştırılmıştır. L. plantarum suşları Fe ve Co’a karşı diğer metallerden daha fazla dirençlilik göstermiştir. Ayrıca L. plantarum PFC19, PFC22, PFC33 ve PFC36 suşlarının genomunda metal dirençlilikle ilişkili Cu-transport ATPaz, PFC7 ve PFC36 suşlarında ise Cd-transport-ATPaz genleri tespit edilmiştir. Bu sonuçlar, L.plantarum PFC7, PFC19, PFC22, PFC33 ve PFC36 suşlarının genetik temelli metal dirençlilik özelliklerinden dolayı olası metal stresine karşı fermente gıdalar için starter kültür olarak uygulama potansiyeli bulunduğunu göstermiştir

Keywords

Laktik asit bakterisi, L. plantarum, Metal dirençlilik, Fermente gıdalar

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