FARKLI HİDROLİK ALIKONMA SÜRELERİNDE KEÇİBOYNUZU EKSTRAKTI BESİYERİNDE SÜREKLİ ETANOL FERMANTASYONU

Year 2019, Volume: 44 Issue: 1, 93 - 103, 15.02.2019

Ercan Yatmaz

https://doi.org/10.15237/gida.GD18085

Abstract

Enerji endüstrisinin tüm dünyada ucuz yenilenebilir
kaynaklar sağlaması için önemli biyoproseslerden birisi biyoetanol üretimidir.
Bu çalışmada serbest veya immobilize edilmiş S. cerevisiae hücreleri ile
ucuz bir karbon kaynağı olan keçiboynuzu ekstraktından sürekli etanol
fermantasyonları amaçlanmıştır. Sürekli etanol fermantasyonları farklı hidrolik
alıkonma sürelerinde (4-20 saat) gerçekleştirilmiştir. Optimum hidrolik
alıkonma süreleri, serbest haldeki hücreler için 8 sa ve immobilize edilmiş
hücreler için 6.67 sa olarak belirlenmiştir. Serbest ve immobilize hücre
fermantasyonları için en yüksek etanol üretim oranları sırasıyla 3.12 g/L/sa ve
3.37 g/L/sa olarak hidrolik alıkonma süresi 5.71 saatte elde edilmiştir. Tüm
kinetik parametreler, her iki hücre tipinin etanol fermantasyonu için
kullanılabileceğini ve immobilize edilmiş S. cerevisiae hücreleri ile
gerçekleştirilen etanol fermantasyonunun, süspansiyon haldeki hücrelere kıyasla
biyokütleden bağımsız olarak daha yüksek seyreltme oranlarında
gerçekleştirilebileceğini açıkça göstermiştir.

Keywords

Sürekli fermantasyon, serbest ve immobilize hücreler, karıştırmalı tank tipi biyoreaktör

CONTINUOUS ETHANOL FERMENTATION FROM CAROB POD EXTRACT MEDIUM AT DIFFERENT HYDRAULIC RESIDENCE TIME (HRT)

Abstract

Production of bioethanol is one
of the important bioprocesses for the energy industry to provide inexpensive
renewable resources all over the world. In this context, this research was
organized for continuous ethanol fermentation from carob pod extract which is
an inexpensive carbon source by free or immobilized S. cerevisiae cells.
Continuous ethanol fermentations were performed with different HRT (from 4 to
20 h) and optimal HRT were 8 h for the free cell, and 6.67 h for immobilized
cell, respectively. The highest volumetric ethanol
productivities for free cell and immobilized cell fermentations were 3.12 g/L/h
and 3.37 g/L/h at HRT of 5.71 h, respectively. All kinetic parameters
clearly showed that both cell types can be used for ethanol fermentation, and
immobilized S. cerevisiae ethanol fermentation can be operated at higher
dilution rates independent of biomass than a free cell.

Keywords

Continuous fermentation, stirred tank bioreactor, free and immobilized cells, stirred tank bioreactor

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