The Investigation of Levels of Synthetic Colorants in some Foodstuffs Sold in Ankara Region, Turkey (Turkish with English Abstract)

Year 2010, Volume: 35 Issue: 1, 1 - 5, 01.02.2010

Gülderen Yentür Şebnem Erdoğan Buket Er Burak Demirhan Aysel Bayhan Öktem

Abstract

The aim of this study was to determine synthetic colorants in the 160 foodstuffs samples including jam, fruit yogurt, fruit juice, non-alcoholic beverages and sugar coated apples sold in Ankara Region (Turkey). The synthetic colorants; Tartrazine, Quinoline Yellow, Sunset Yellow F.C.F, Carmoisine, Ponceau 4R, Indigotin were determined qualitatively in all samples using wool coloring and paper chromatography methods. First-derivative spectrophotometric method was used for quantitative determination. Mean level of Ponceau 4R together with Carmoisine was found to be 49.2405±0.0002 mg/l in 11 non-alcoholic beverage samples. Mean levels of Ponceau 4R in the sugar coated apple samples of A and B pastry-shops were found as 2265.3140±36.0215 mg/kg and 584.4700±3.5281 mg/kg, respectively. Our data revealed that while synthetic colorants found in non-alcoholic beverage samples were within the limits of Turkish Food Codex (TFC), Ponceau 4R levels in sugar coated apple samples were above the TFC limits. Synthetic colorants were not found in other food and beverage samples.

Keywords

Food colorants, food additives, derivative spectrophotometry

Ankara Bölgesinde Tüketime Sunulan Bazı Gıda Maddelerinde Sentetik Boya Düzeylerinin Araştırılması

Abstract

Bu çalışmada Ankara piyasasından sağlanan reçel, meyveli yoğurt, meyve suyu, alkolsüz içecek, elmalı şeker örneklerinden oluşan toplam 160 gıda maddesinde bazı sentetik boyaların saptanması amaçlanmıştır. Bütün örneklerde Tartrazine, Quinoline Yellow, Sunset Yellow F.C.F, Carmoisine, Ponceau 4R, Indigotin sentetik boya maddeleri yün boyama ve kâğıt kromatografisi yöntemiyle kalitatif olarak saptanmıştır. Kantitatif tayin birinci dereceden türev spektrofotometresi yöntemi kullanılarak yapılmıştır. 11 adet alkolsüz içecek örneğinde Ponceau 4R ve Carmoisine boyalarının ortalama değeri 49.2405±0.0002 mg/l olarak bulunmuştur. A ve B pastanelerine ait elmalı şekerlerde Ponceau 4R’nin ortalama düzeyleri sırasıyla 2265.3140±36.0215 mg/kg ve 584.4700±3.5281 mg/kg olarak saptanmıştır. Elde edilen bulgulara göre alkolsüz içeceklerdeki sentetik boya miktarlarının Türk Gıda Kodeksinin (TGK) belirttiği sınırları aşmadığı ve elmalı şekerlerdeki Ponceau 4R miktarlarının ise Türk Gıda Kodeksinin sınır değerlerinin üzerinde olduğu bulunmuştur. Diğer gıda ve içecek örneklerinde ise sentetik boya varlığına rastlanmamıştır.

Keywords

Gıda boyaları, gıda katkı maddeleri, türev spektrofotometrisi　　

References

• Altınöz S, Toptan S. 2003. Simultaneous determina- tion of Indigotin and Ponceau-4R in food samples by using Vierordt’s method, ratio spectra first order deriva- tive and derivative UV spectrophotometry. J Food Com- pos Anal, 16, 517–530.
• Minioti KS, Sakellariou CF, Thomaidis, NS. 2007.
• Determination of 13 synthetic food colorants in water- soluble foods by reversed-phase high-performance liqu- id chromatography coupled with diode-array detector. Anal Chim Acta, 583, 103–110. Tripathi M, Khanna SK and DAS, M. 2007. Surveillan- ce on use of synthetic colours in eatables vis a vis pre- vention of food adulteration act of India. Food Control, , 211–219.
• Gennaro MC, Gioannini E, Angelino S, Aigotti, R. Giacosa D. 1997. Identification and determination of red dyes in confectionery by ion-interaction high- performance liquid chromatography. J Chromatogr A, , 87-92.
• Nevado JJB, Flores JR, Cabanillas CG, Llerena MJ, Sal- cedo. 1998. Resolution of ternary mixtures of Tartrazine,
• Sunset Yellow and Ponceau 4R by derivative spectropho- tometric ratio spectrum-zero crossing method in com- mercial. Talanta, 46, 933-942. Özdemir Y, Akkan A. 1999. Determination of Patent
• Blue V and Carmoisine in gelatie desserts by derivative spectrophotometry. Turk J Chem, 23, 221-229. Perez-Urquiza M. and Beltran JL. 2000. Determinati- on of dyes in foodstuffs by capillary zone electrophore- sis. J Chromatogr A, 898, 271–275.
• Del Giovine L, Piccioli Boca A. 2003. Determination of synthetic dyes in ice-cream by capillary electrophore- sis. Food Control, 14, 131–135.
• Chanlon S, Joly-Pottuz L, Chatelut M, Vittori O, Cre- tier JL. 2005. Determination of Carmoisine, Allura red and Ponceau 4R in sweets and soft drinks by Differenti- al Pulse Polarography. J Food Compos Anal, 18, 503–515.
• Garcia-Falcon MS. & Simal-Gandara J. 2005. Deter- mination of food dyes in soft drinks containing natural pigments by liquid chromatography with minimal clean- up. Food Control, 16 (3), 293-297.
• Elhkim MO, He´Raud F, Bemrah N, Gauchard F, Lo- rina T, Lambre C, Fre´My JM, Poul JM. 2007. New con- siderations regarding the risk assessment on Tartrazine an update toxicological assessment, intolerance reacti- ons and maximum theoretical daily intake in France. Re- gul Toxicol Pharm, 47, 308–316.
• Ram FS, Ardern KD.  2007. Tartrazine exclusion for allergic asthma. Cochrane DB Syst Rev, Issue 4. Art. No.: CD000460. DOI: 10.1002/14651858. CD 000460.
• Jaworska M, Szulinska Z, Wilk M, Anuszewska E. Separation of synthetic food colourants in the mixed micellar system application to pharmaceutical analysis. J Chromatogr A, 1081, 42–47.
• Ahlström LH, Eskilsson CS, Björklund E. 2005. De- termination of banned azo dyes in consumer goods.
• Trend Anal Chem, 24 (1), 49-56. Anon 1994. On colours for use in foodstuffs, L 237/13
• EN Official Journal of the European Communities, Euro- pean Parliament and council directive (EC) No 94/36 of June 1994.
• Anon 1995. Laying down specific purity criteria con- cerning colours for use in foodstuffs, L226 EN Official
• Journal of the European Communities, Commission di- rective (EC) No 95/45 of 26 July 1995.
• Anon 2002. Türk Gıda Kodeksi. Gıdalarda Kullanı- lan Renklendiriciler Tebliği (2002/55). Tarım ve Köyişle- ri Bakanlığı ve Sağlık Bakanlığı. 16 Kasım 1997 tarih ve sayılı Resmi Gazete, Ankara .
• Woodman AG. 1941. Food Analysis – Typical Met- hods and the Interpretation of Results. Fourth Ed.,
• McGraw-Hill Book Company, London, 70 – 72 p. Lees R. 1968. Laboratory Handbook of Methods of
• Food Analysis, Croc Press, Cleveland, 102-103 p. Fell AF, Allan JG. 1981. Analysis of colouring agents in pharmaceuticals by derivative ultraviolet – visible spectroscopy, Anal Commun, 291-296 p.
• Nevado JJB, Flores JR, Llerena MJV. 1993. Simulta- neous determination of Tartrazine and Sunset Yellow by
• Derivative Spectrophotometry and Ratio Spectra Deri- vative. Talanta. 40 (9), 1391-1396.
• Daniel NW. 1987. Biostatistics, 5th Edition, John Wi- ley and Sons, New York, 740 p.
• Yentür G, Karakaya, AE. 1985. Kullanımı yasakla- nan aromatik azo yapısındaki gıda boyalarının bazı gıda maddelerinde araştırılması. Gıda, 10 (6): 371-376.
• Yentür G, Ekşi A, Bayhan A. 1996. Ankara piyasa- sından sağlanan pasta süsleri ve bazı şekerlerde sentetik boya miktarlarının araştırılması. Ankara Üniv. Vet. Fak. Derg, 43 (4): 479-484.
• Üstün NŞ, Tosun İ. 1998. Çeşitli reçellerin bileşimi üzerine bir araştırma, Gıda, 23(2): 125-131.
• Topsoy H. 1998. Ankara piyasasında satılan bazı şe- ker ve şekerli gıda maddelerine katılan gıda boyalarının türev spektroskopi yöntemi ile kantitatif tayini, Ankara
• Üniversitesi Sağlık Bilimleri Enstitüsü Besin Hijyeni ve Teknolojisi Ana Bilim Dalı, Doktora Tezi, Ankara, Tür- kiye. Yentür G, Yaman M, Bayhan A. 1998. Bazı gıda mad- delerine katılan sentetik boyaların miktarlarının araştı- rılması. Gıda, 23(3): 195-199.
• Jonnalagadda PR, Rao P, Bhat RV, Naidu AN. 2004.
• Type, extend and use of colours in ready-to-eat foods prepared in the non-industrial sector–a case study from Hyderabad, India. Int J Food Sci Tech, 39 (2), 125- Lau O, Poon MMK, Siu CM, Wong FMY. 1996.
• Spectrophotometric determination of single synthetic food colour in soft drinks, using ion-pair formation and extraction. Int J Food Sci Tech, 30 (6), 793-798. Biswas G, Sarkar S, Chatterjee TK. 1994. Surveil- lance on artificial colours in food products marketed in Calcuta and Adjoining Areas. J Food Sci Tech, 31 (1), 67.